Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments
The increasing interest toward biocompatible nanotechnologies in medicine, combined with electric fields stimulation, is leading to the development of electro-sensitive smart systems for drug delivery applications. To this regard, recently the use of pulsed electric fields to trigger release across...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00819/full |
| _version_ | 1818007967010652160 |
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| author | Laura Caramazza Laura Caramazza Martina Nardoni Annalisa De Angelis Patrizia Paolicelli Micaela Liberti Micaela Liberti Francesca Apollonio Francesca Apollonio Stefania Petralito |
| author_facet | Laura Caramazza Laura Caramazza Martina Nardoni Annalisa De Angelis Patrizia Paolicelli Micaela Liberti Micaela Liberti Francesca Apollonio Francesca Apollonio Stefania Petralito |
| author_sort | Laura Caramazza |
| collection | DOAJ |
| description | The increasing interest toward biocompatible nanotechnologies in medicine, combined with electric fields stimulation, is leading to the development of electro-sensitive smart systems for drug delivery applications. To this regard, recently the use of pulsed electric fields to trigger release across phospholipid membranes of liposomes has been numerically studied, for a deeper understanding of the phenomena at the molecular scale. Aim of this work is to give an experimental validation of the feasibility to control the release from liposome vesicles, using nanosecond pulsed electric fields characterized by a 10 ns duration and intensity in the order of MV/m. The results are supported by multiphysics simulations which consider the coupling of three physics (electromagnetics, thermal and pore kinetics) in order to explain the occurring physical interactions at the microscopic level and provide useful information on the characteristics of the train of pulses needed to obtain quantitative results in terms of liposome electropermeabilization. Finally, a complete characterization of the exposure system is also provided to support the reliability and validity of the study. |
| first_indexed | 2024-04-14T05:22:35Z |
| format | Article |
| id | doaj.art-89eb3068538a44cfb120a41f76bc7d8e |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-14T05:22:35Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-89eb3068538a44cfb120a41f76bc7d8e2022-12-22T02:10:08ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-07-01810.3389/fbioe.2020.00819552304Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet ExperimentsLaura Caramazza0Laura Caramazza1Martina Nardoni2Annalisa De Angelis3Patrizia Paolicelli4Micaela Liberti5Micaela Liberti6Francesca Apollonio7Francesca Apollonio8Stefania Petralito9ICEmB at DIET, Sapienza University of Rome, Rome, ItalyCenter for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, ItalyDepartment of Drug Chemistry and Technologies, Sapienza University of Rome, Rome, ItalyCenter for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, ItalyDepartment of Drug Chemistry and Technologies, Sapienza University of Rome, Rome, ItalyICEmB at DIET, Sapienza University of Rome, Rome, ItalyCenter for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, ItalyICEmB at DIET, Sapienza University of Rome, Rome, ItalyCenter for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Rome, ItalyDepartment of Drug Chemistry and Technologies, Sapienza University of Rome, Rome, ItalyThe increasing interest toward biocompatible nanotechnologies in medicine, combined with electric fields stimulation, is leading to the development of electro-sensitive smart systems for drug delivery applications. To this regard, recently the use of pulsed electric fields to trigger release across phospholipid membranes of liposomes has been numerically studied, for a deeper understanding of the phenomena at the molecular scale. Aim of this work is to give an experimental validation of the feasibility to control the release from liposome vesicles, using nanosecond pulsed electric fields characterized by a 10 ns duration and intensity in the order of MV/m. The results are supported by multiphysics simulations which consider the coupling of three physics (electromagnetics, thermal and pore kinetics) in order to explain the occurring physical interactions at the microscopic level and provide useful information on the characteristics of the train of pulses needed to obtain quantitative results in terms of liposome electropermeabilization. Finally, a complete characterization of the exposure system is also provided to support the reliability and validity of the study.https://www.frontiersin.org/article/10.3389/fbioe.2020.00819/fullnanosecond pulsed electric fieldsliposome vesiclescontrolled releaseelectroporationelectropermeabilizationexposure systems |
| spellingShingle | Laura Caramazza Laura Caramazza Martina Nardoni Annalisa De Angelis Patrizia Paolicelli Micaela Liberti Micaela Liberti Francesca Apollonio Francesca Apollonio Stefania Petralito Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments Frontiers in Bioengineering and Biotechnology nanosecond pulsed electric fields liposome vesicles controlled release electroporation electropermeabilization exposure systems |
| title | Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments |
| title_full | Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments |
| title_fullStr | Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments |
| title_full_unstemmed | Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments |
| title_short | Proof-of-Concept of Electrical Activation of Liposome Nanocarriers: From Dry to Wet Experiments |
| title_sort | proof of concept of electrical activation of liposome nanocarriers from dry to wet experiments |
| topic | nanosecond pulsed electric fields liposome vesicles controlled release electroporation electropermeabilization exposure systems |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00819/full |
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