Adopting oculopressure tonometry as a transient in vivo rabbit glaucoma model
Glaucoma represents a group of eye disorders partly related to raised intraocular pressure (IOP) leading to progressive optic nerve damage resulting in impaired vision and possibly blindness. To assess the suitability of new IOP lowering therapeutic strategies, such as the implantation of glaucoma d...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2015-09-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2015-0033 |
| _version_ | 1818720618602823680 |
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| author | Stahnke T. Siewert S. Walther E. Schmidt W. Stachs O. Schmitz K.-P. Guthoff R. F. |
| author_facet | Stahnke T. Siewert S. Walther E. Schmidt W. Stachs O. Schmitz K.-P. Guthoff R. F. |
| author_sort | Stahnke T. |
| collection | DOAJ |
| description | Glaucoma represents a group of eye disorders partly related to raised intraocular pressure (IOP) leading to progressive optic nerve damage resulting in impaired vision and possibly blindness. To assess the suitability of new IOP lowering therapeutic strategies, such as the implantation of glaucoma drainage devices, appropriate animal models have to be used. Currently, a number of rodent glaucoma models are available [1], however, especially for surgical interventions rodent eyes are too small. Rabbits are much more suitable with respect to dimension. Unfortunately, rabbit glaucoma model systems described in literature are difficult to reproduce or fail totally, associated with a high level of discomfort and pain for treated animals. Therefore, development of an in vivo rabbit glaucoma model is one of the most important goals in glaucoma research. Here, we describe the adaptation of the oculopressure tonometry, an existing method to quantify the outflow of aqueous humor in humans, to generate a transient glaucoma model in rabbits. The existing suction-cup oculopressor (SCOP) is extended with newly designed suction-cups, which are adjusted to the anatomy of the rabbit eye. The modification of the oculopressure tonometry method facilitates an increase in IOP over a time frame of 9 minutes by vacuum induced deformation of the rabbit eye. This method can be used to test functionality of fistulating glaucoma surgeries or implanted drainage devices in a long term follow-up without any side effects and suffering of the animals. |
| first_indexed | 2024-12-17T20:25:42Z |
| format | Article |
| id | doaj.art-89f32ae22dcb431ebd11e2ec9be27ffb |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-12-17T20:25:42Z |
| publishDate | 2015-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-89f32ae22dcb431ebd11e2ec9be27ffb2022-12-21T21:33:47ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042015-09-011112713010.1515/cdbme-2015-0033cdbme-2015-0033Adopting oculopressure tonometry as a transient in vivo rabbit glaucoma modelStahnke T.0Siewert S.1Walther E.2Schmidt W.3Stachs O.4Schmitz K.-P.5Guthoff R. F.6Institute for Biomedical Engineering, Rostock University Medical Center, Rostock, Germany, Phone: +49-38154945547; Fax: +49-38154945502Institute for Biomedical Engineering, Rostock University Medical Center, Rostock, GermanyDepartment of Ophthalmology, Rostock University Medical Center, Rostock, GermanyInstitute for Biomedical Engineering, Rostock University Medical Center, Rostock, GermanyDepartment of Ophthalmology, Rostock University Medical Center, Rostock, GermanyInstitute for Biomedical Engineering, Rostock University Medical Center, Rostock, GermanyInstitute for Biomedical Engineering, Rostock University Medical Center, Rostock, GermanyGlaucoma represents a group of eye disorders partly related to raised intraocular pressure (IOP) leading to progressive optic nerve damage resulting in impaired vision and possibly blindness. To assess the suitability of new IOP lowering therapeutic strategies, such as the implantation of glaucoma drainage devices, appropriate animal models have to be used. Currently, a number of rodent glaucoma models are available [1], however, especially for surgical interventions rodent eyes are too small. Rabbits are much more suitable with respect to dimension. Unfortunately, rabbit glaucoma model systems described in literature are difficult to reproduce or fail totally, associated with a high level of discomfort and pain for treated animals. Therefore, development of an in vivo rabbit glaucoma model is one of the most important goals in glaucoma research. Here, we describe the adaptation of the oculopressure tonometry, an existing method to quantify the outflow of aqueous humor in humans, to generate a transient glaucoma model in rabbits. The existing suction-cup oculopressor (SCOP) is extended with newly designed suction-cups, which are adjusted to the anatomy of the rabbit eye. The modification of the oculopressure tonometry method facilitates an increase in IOP over a time frame of 9 minutes by vacuum induced deformation of the rabbit eye. This method can be used to test functionality of fistulating glaucoma surgeries or implanted drainage devices in a long term follow-up without any side effects and suffering of the animals.https://doi.org/10.1515/cdbme-2015-0033glaucomaoculopressure tonometry (opt)rabbit glaucoma modelintraocular pressure (iop) |
| spellingShingle | Stahnke T. Siewert S. Walther E. Schmidt W. Stachs O. Schmitz K.-P. Guthoff R. F. Adopting oculopressure tonometry as a transient in vivo rabbit glaucoma model Current Directions in Biomedical Engineering glaucoma oculopressure tonometry (opt) rabbit glaucoma model intraocular pressure (iop) |
| title | Adopting oculopressure tonometry as a transient in vivo rabbit glaucoma model |
| title_full | Adopting oculopressure tonometry as a transient in vivo rabbit glaucoma model |
| title_fullStr | Adopting oculopressure tonometry as a transient in vivo rabbit glaucoma model |
| title_full_unstemmed | Adopting oculopressure tonometry as a transient in vivo rabbit glaucoma model |
| title_short | Adopting oculopressure tonometry as a transient in vivo rabbit glaucoma model |
| title_sort | adopting oculopressure tonometry as a transient in vivo rabbit glaucoma model |
| topic | glaucoma oculopressure tonometry (opt) rabbit glaucoma model intraocular pressure (iop) |
| url | https://doi.org/10.1515/cdbme-2015-0033 |
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