Use of stem sliced from in vitro plants of papaya (hybrid IBP 42-99) by obtained callus with embryogenic structure
Inside in vitro propagation via, the somatic embriogenesis offers possibilities of obtaining top volumes of production in a minor period of time and a lower cost, which are a method potentially more efficient than the regeneration via organogenesis. In papaya the somatic embryogenesis could have dev...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidad Central Marta Abreu de Las Villas
2004-10-01
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| Series: | Biotecnología Vegetal |
| Online Access: | https://revista.ibp.co.cu/index.php/BV/article/view/429 |
| _version_ | 1818513841610293248 |
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| author | Jorge Gallardo Colina Rafael Gómez Kosky Marisol Tejeda Fernández Laisyn Posada Pérez Idalia Herrera O´farril Maritza Reyes Vega Leyanis García Aguila Marisol Freire Seijo |
| author_facet | Jorge Gallardo Colina Rafael Gómez Kosky Marisol Tejeda Fernández Laisyn Posada Pérez Idalia Herrera O´farril Maritza Reyes Vega Leyanis García Aguila Marisol Freire Seijo |
| author_sort | Jorge Gallardo Colina |
| collection | DOAJ |
| description | Inside in vitro propagation via, the somatic embriogenesis offers possibilities of obtaining top volumes of production in a minor period of time and a lower cost, which are a method potentially more efficient than the regeneration via organogenesis. In papaya the somatic embryogenesis could have developed from zigotic embryos and axis hipocotilos, nevertheless in case of the hybrids these methods cannot be used and it becomes necessary to develop it from a somatic fabric, without link with the sexual reproduction. This work chased as main objective Evaluated the use of in vitro plants stem sections of the Carica papaya IBP 42-99 hybrid for the formation of callus with embryogenic structures. As plant material were use in vitro plants of the papaya hybrid IBP 42-99. For it there took sections of different parts of the stem from the meristem up to the base of the in vitro plants, was use the culture medium Nitsh and Nitsh supplemented with 1.5 mg.l-1 of 6-BAP and 1.5 mg.l-1 of AIA. It was achieved to obtain callus from the stem sections with the culture medium used, nevertheless, in the treatments where used cylinders inside 1.0 cm from the apex down, the best results were achieved. The use of in vitro plants stem sections as explant for the formation of callus in this vegetable species it opens new possibilities for his in vitro propagation, specially in case of resultant hybrids of genetic improvement programs.
Key words: apexes, Carica papaya, callogenesis, somatic embryogenesis |
| first_indexed | 2024-12-11T00:07:06Z |
| format | Article |
| id | doaj.art-89f5ae71d5f243d79ef41dc7b796eb2f |
| institution | Directory Open Access Journal |
| issn | 1609-1841 2074-8647 |
| language | English |
| last_indexed | 2024-12-11T00:07:06Z |
| publishDate | 2004-10-01 |
| publisher | Universidad Central Marta Abreu de Las Villas |
| record_format | Article |
| series | Biotecnología Vegetal |
| spelling | doaj.art-89f5ae71d5f243d79ef41dc7b796eb2f2022-12-22T01:28:17ZengUniversidad Central Marta Abreu de Las VillasBiotecnología Vegetal1609-18412074-86472004-10-0144396Use of stem sliced from in vitro plants of papaya (hybrid IBP 42-99) by obtained callus with embryogenic structureJorge Gallardo ColinaRafael Gómez KoskyMarisol Tejeda FernándezLaisyn Posada PérezIdalia Herrera O´farrilMaritza Reyes VegaLeyanis García AguilaMarisol Freire SeijoInside in vitro propagation via, the somatic embriogenesis offers possibilities of obtaining top volumes of production in a minor period of time and a lower cost, which are a method potentially more efficient than the regeneration via organogenesis. In papaya the somatic embryogenesis could have developed from zigotic embryos and axis hipocotilos, nevertheless in case of the hybrids these methods cannot be used and it becomes necessary to develop it from a somatic fabric, without link with the sexual reproduction. This work chased as main objective Evaluated the use of in vitro plants stem sections of the Carica papaya IBP 42-99 hybrid for the formation of callus with embryogenic structures. As plant material were use in vitro plants of the papaya hybrid IBP 42-99. For it there took sections of different parts of the stem from the meristem up to the base of the in vitro plants, was use the culture medium Nitsh and Nitsh supplemented with 1.5 mg.l-1 of 6-BAP and 1.5 mg.l-1 of AIA. It was achieved to obtain callus from the stem sections with the culture medium used, nevertheless, in the treatments where used cylinders inside 1.0 cm from the apex down, the best results were achieved. The use of in vitro plants stem sections as explant for the formation of callus in this vegetable species it opens new possibilities for his in vitro propagation, specially in case of resultant hybrids of genetic improvement programs. Key words: apexes, Carica papaya, callogenesis, somatic embryogenesishttps://revista.ibp.co.cu/index.php/BV/article/view/429 |
| spellingShingle | Jorge Gallardo Colina Rafael Gómez Kosky Marisol Tejeda Fernández Laisyn Posada Pérez Idalia Herrera O´farril Maritza Reyes Vega Leyanis García Aguila Marisol Freire Seijo Use of stem sliced from in vitro plants of papaya (hybrid IBP 42-99) by obtained callus with embryogenic structure Biotecnología Vegetal |
| title | Use of stem sliced from in vitro plants of papaya (hybrid IBP 42-99) by obtained callus with embryogenic structure |
| title_full | Use of stem sliced from in vitro plants of papaya (hybrid IBP 42-99) by obtained callus with embryogenic structure |
| title_fullStr | Use of stem sliced from in vitro plants of papaya (hybrid IBP 42-99) by obtained callus with embryogenic structure |
| title_full_unstemmed | Use of stem sliced from in vitro plants of papaya (hybrid IBP 42-99) by obtained callus with embryogenic structure |
| title_short | Use of stem sliced from in vitro plants of papaya (hybrid IBP 42-99) by obtained callus with embryogenic structure |
| title_sort | use of stem sliced from in vitro plants of papaya hybrid ibp 42 99 by obtained callus with embryogenic structure |
| url | https://revista.ibp.co.cu/index.php/BV/article/view/429 |
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