Follicular Atresia, Cell Proliferation, and Anti-Mullerian Hormone in Two Neotropical Primates (<i>Aotus nancymae</i> and <i>Sapajus macrocephalus</i>)
This study evaluated the follicular atresia, cell proliferation, and anti-Mullerian hormone action in <i>Aotus nancymae</i> and <i>Sapajus macrocephalus</i> during three sexual phases (follicular, luteal, and gestational). Follicular quantification and immunolocalization of C...
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| Format: | Article |
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MDPI AG
2023-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/6/1051 |
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| author | Deise de Lima Cardoso Diva Anélie de Araújo Guimarães Pedro Mayor Maria Auxiliadora Pantoja Ferreira Leandro Nassar Coutinho Frederico Ozanan Barros Monteiro |
| author_facet | Deise de Lima Cardoso Diva Anélie de Araújo Guimarães Pedro Mayor Maria Auxiliadora Pantoja Ferreira Leandro Nassar Coutinho Frederico Ozanan Barros Monteiro |
| author_sort | Deise de Lima Cardoso |
| collection | DOAJ |
| description | This study evaluated the follicular atresia, cell proliferation, and anti-Mullerian hormone action in <i>Aotus nancymae</i> and <i>Sapajus macrocephalus</i> during three sexual phases (follicular, luteal, and gestational). Follicular quantification and immunolocalization of Caspase-3 protein, B-cell lymphoma 2 (BCL-2), proliferating cell nuclear antigen (PCNA), and anti-Mullerian hormone (AMH) were performed. A significant difference in the quantification between preantral and antral follicles, with a progressive decrease in the antrals, was identified. Protein and hormonal markers varied significantly between follicle cell types (<i>A. nancymae p</i> = 0.001; <i>S. macrocephalus</i>, <i>p</i> = 0.002). Immunostaining in the preantral and antral follicles was present in all sexual phases; for Caspase-3, in granulosa cells, oocytes, and stroma; for BCL-2, in granulosa cells, oocytes, and theca; and for PCNA and AMH, in oocytes and granulosa cells. The immunostaining for Caspase-3 was more expressive in the preantral follicles (follicular phase, <i>p</i> < 0.05), while that for BCL-2 and PCNA was more expressive in the antral follicles of the follicular phase. The AMH was more expressive in the primary and antral follicles of nonpregnant females, in both the follicular and luteal phases. Our results contribute to understanding the ovarian follicular selection, recruitment, and degeneration of these species. |
| first_indexed | 2024-03-11T07:01:39Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T07:01:39Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-89f63a89c4374f9d8eb0974d088162732023-11-17T09:11:38ZengMDPI AGAnimals2076-26152023-03-01136105110.3390/ani13061051Follicular Atresia, Cell Proliferation, and Anti-Mullerian Hormone in Two Neotropical Primates (<i>Aotus nancymae</i> and <i>Sapajus macrocephalus</i>)Deise de Lima Cardoso0Diva Anélie de Araújo Guimarães1Pedro Mayor2Maria Auxiliadora Pantoja Ferreira3Leandro Nassar Coutinho4Frederico Ozanan Barros Monteiro5Programa de pós-graduação em Ciência Animal, Universidade Federal do Pará, Rua Augusto Corrêa 01, Belém 66075-110, BrazilPrograma de pós-graduação em Ciência Animal, Universidade Federal do Pará, Rua Augusto Corrêa 01, Belém 66075-110, BrazilPrograma de Pós-Graduação em Saúde e Produção Animal na Amazônia, Universidade Federal Rural da Amazônia, Av. Perimetral 2501, Belém 66077-830, BrazilPrograma de pós-graduação em Ciência Animal, Universidade Federal do Pará, Rua Augusto Corrêa 01, Belém 66075-110, BrazilPrograma de Pós-Graduação em Saúde e Produção Animal na Amazônia, Universidade Federal Rural da Amazônia, Av. Perimetral 2501, Belém 66077-830, BrazilPrograma de Pós-Graduação em Saúde e Produção Animal na Amazônia, Universidade Federal Rural da Amazônia, Av. Perimetral 2501, Belém 66077-830, BrazilThis study evaluated the follicular atresia, cell proliferation, and anti-Mullerian hormone action in <i>Aotus nancymae</i> and <i>Sapajus macrocephalus</i> during three sexual phases (follicular, luteal, and gestational). Follicular quantification and immunolocalization of Caspase-3 protein, B-cell lymphoma 2 (BCL-2), proliferating cell nuclear antigen (PCNA), and anti-Mullerian hormone (AMH) were performed. A significant difference in the quantification between preantral and antral follicles, with a progressive decrease in the antrals, was identified. Protein and hormonal markers varied significantly between follicle cell types (<i>A. nancymae p</i> = 0.001; <i>S. macrocephalus</i>, <i>p</i> = 0.002). Immunostaining in the preantral and antral follicles was present in all sexual phases; for Caspase-3, in granulosa cells, oocytes, and stroma; for BCL-2, in granulosa cells, oocytes, and theca; and for PCNA and AMH, in oocytes and granulosa cells. The immunostaining for Caspase-3 was more expressive in the preantral follicles (follicular phase, <i>p</i> < 0.05), while that for BCL-2 and PCNA was more expressive in the antral follicles of the follicular phase. The AMH was more expressive in the primary and antral follicles of nonpregnant females, in both the follicular and luteal phases. Our results contribute to understanding the ovarian follicular selection, recruitment, and degeneration of these species.https://www.mdpi.com/2076-2615/13/6/1051owl monkeynight monkeycapuchin monkeyovaryfolliculogenesisapoptosis |
| spellingShingle | Deise de Lima Cardoso Diva Anélie de Araújo Guimarães Pedro Mayor Maria Auxiliadora Pantoja Ferreira Leandro Nassar Coutinho Frederico Ozanan Barros Monteiro Follicular Atresia, Cell Proliferation, and Anti-Mullerian Hormone in Two Neotropical Primates (<i>Aotus nancymae</i> and <i>Sapajus macrocephalus</i>) Animals owl monkey night monkey capuchin monkey ovary folliculogenesis apoptosis |
| title | Follicular Atresia, Cell Proliferation, and Anti-Mullerian Hormone in Two Neotropical Primates (<i>Aotus nancymae</i> and <i>Sapajus macrocephalus</i>) |
| title_full | Follicular Atresia, Cell Proliferation, and Anti-Mullerian Hormone in Two Neotropical Primates (<i>Aotus nancymae</i> and <i>Sapajus macrocephalus</i>) |
| title_fullStr | Follicular Atresia, Cell Proliferation, and Anti-Mullerian Hormone in Two Neotropical Primates (<i>Aotus nancymae</i> and <i>Sapajus macrocephalus</i>) |
| title_full_unstemmed | Follicular Atresia, Cell Proliferation, and Anti-Mullerian Hormone in Two Neotropical Primates (<i>Aotus nancymae</i> and <i>Sapajus macrocephalus</i>) |
| title_short | Follicular Atresia, Cell Proliferation, and Anti-Mullerian Hormone in Two Neotropical Primates (<i>Aotus nancymae</i> and <i>Sapajus macrocephalus</i>) |
| title_sort | follicular atresia cell proliferation and anti mullerian hormone in two neotropical primates i aotus nancymae i and i sapajus macrocephalus i |
| topic | owl monkey night monkey capuchin monkey ovary folliculogenesis apoptosis |
| url | https://www.mdpi.com/2076-2615/13/6/1051 |
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