Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of Rats
The dietary intake of fatty acids (FAs) affects the composition and distribution of FAs in the body. Here, a first-generation (n-3)-deficiency study was conducted by keeping young (age 21 ± 2 days) Sprague–Dawley male rats on a peanut-oil-based diet for 33 days after weaning in order to compare the...
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MDPI AG
2022-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/2/208 |
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| author | Amruta Kulkarni Ai Zhao Baoru Yang Yumei Zhang Kaisa M. Linderborg |
| author_facet | Amruta Kulkarni Ai Zhao Baoru Yang Yumei Zhang Kaisa M. Linderborg |
| author_sort | Amruta Kulkarni |
| collection | DOAJ |
| description | The dietary intake of fatty acids (FAs) affects the composition and distribution of FAs in the body. Here, a first-generation (n-3)-deficiency study was conducted by keeping young (age 21 ± 2 days) Sprague–Dawley male rats on a peanut-oil-based diet for 33 days after weaning in order to compare the effect of mild (n-3)-deficiency on the lipid composition of different organs and feces. Soybean-oil-based diet was used as a control. The plasma FA levels corresponded to FAs levels in the organs. Lower docosahexaenoic acid (DHA) content was detected in the plasma, brain, testis, visceral fat, heart, and lungs of the (n-3)-deficient group, whereas the DHA content of the eye and feces did not differ between the experimental groups. The DHA content of the brains of the (n-3)-deficient group was 86% of the DHA content of the brains of the (n-3)-adequate group. The DHA level of the organs was affected in the order of visceral fat > liver triacylglycerols > lung > heart > liver phospholipids > testis > eye > brain, with brain being least affected. The low levels of (n-3) FAs in the liver, brain, eye, heart, and lung were offset by an increase in the (n-6) FAs, mainly arachidonic acid. These results indicate that, in rats, adequate maternal nutrition during pregnancy and weaning does not provide enough (n-3) FAs for 33 days of an (n-3)-deficient diet. Results of this study can be used also to evaluate the conditions needed to reach mild (n-3) deficiency in the first generation of rats and to evaluate the feasibility to collect data from a variety of organs or only selected ones. |
| first_indexed | 2024-03-10T01:28:50Z |
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| id | doaj.art-89fa822ddeab4d9f8edb824ead3fb182 |
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| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T01:28:50Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-89fa822ddeab4d9f8edb824ead3fb1822023-11-23T13:45:29ZengMDPI AGFoods2304-81582022-01-0111220810.3390/foods11020208Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of RatsAmruta Kulkarni0Ai Zhao1Baoru Yang2Yumei Zhang3Kaisa M. Linderborg4Food Chemistry and Food Development, Department of Life Technologies, University of Turku, 20520 Turku, FinlandVanke School of Public Health, Tsinghua University, Beijing 100083, ChinaFood Chemistry and Food Development, Department of Life Technologies, University of Turku, 20520 Turku, FinlandDepartment of Nutrition & Food Hygiene, School of Public Health, Peking University Health Science Center, Beijing 100191, ChinaFood Chemistry and Food Development, Department of Life Technologies, University of Turku, 20520 Turku, FinlandThe dietary intake of fatty acids (FAs) affects the composition and distribution of FAs in the body. Here, a first-generation (n-3)-deficiency study was conducted by keeping young (age 21 ± 2 days) Sprague–Dawley male rats on a peanut-oil-based diet for 33 days after weaning in order to compare the effect of mild (n-3)-deficiency on the lipid composition of different organs and feces. Soybean-oil-based diet was used as a control. The plasma FA levels corresponded to FAs levels in the organs. Lower docosahexaenoic acid (DHA) content was detected in the plasma, brain, testis, visceral fat, heart, and lungs of the (n-3)-deficient group, whereas the DHA content of the eye and feces did not differ between the experimental groups. The DHA content of the brains of the (n-3)-deficient group was 86% of the DHA content of the brains of the (n-3)-adequate group. The DHA level of the organs was affected in the order of visceral fat > liver triacylglycerols > lung > heart > liver phospholipids > testis > eye > brain, with brain being least affected. The low levels of (n-3) FAs in the liver, brain, eye, heart, and lung were offset by an increase in the (n-6) FAs, mainly arachidonic acid. These results indicate that, in rats, adequate maternal nutrition during pregnancy and weaning does not provide enough (n-3) FAs for 33 days of an (n-3)-deficient diet. Results of this study can be used also to evaluate the conditions needed to reach mild (n-3) deficiency in the first generation of rats and to evaluate the feasibility to collect data from a variety of organs or only selected ones.https://www.mdpi.com/2304-8158/11/2/208docosahexaenoic acid(n-3) polyunsaturated fatty acids(n-3) deficiencyα-linolenic acidlipid metabolismfatty acid composition |
| spellingShingle | Amruta Kulkarni Ai Zhao Baoru Yang Yumei Zhang Kaisa M. Linderborg Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of Rats Foods docosahexaenoic acid (n-3) polyunsaturated fatty acids (n-3) deficiency α-linolenic acid lipid metabolism fatty acid composition |
| title | Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of Rats |
| title_full | Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of Rats |
| title_fullStr | Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of Rats |
| title_full_unstemmed | Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of Rats |
| title_short | Tissue-Specific Content of Polyunsaturated Fatty Acids in (n-3) Deficiency State of Rats |
| title_sort | tissue specific content of polyunsaturated fatty acids in n 3 deficiency state of rats |
| topic | docosahexaenoic acid (n-3) polyunsaturated fatty acids (n-3) deficiency α-linolenic acid lipid metabolism fatty acid composition |
| url | https://www.mdpi.com/2304-8158/11/2/208 |
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