Breeding from 1891 to 2010 did not increase the content of amylase/trypsin-inhibitors in wheat (Triticum aestivum)
Abstract The prevalence of hypersensitivities towards wheat has increased in the last decades. Apart from celiac disease these include allergic and other inflammatory reactions summarized under the term non-celiac wheat sensitivity. One suspected trigger is the family of amylase/trypsin-inhibitors (...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-08-01
|
| Series: | npj Science of Food |
| Online Access: | https://doi.org/10.1038/s41538-023-00219-w |
| _version_ | 1797451346780094464 |
|---|---|
| author | Sabrina Geisslitz Darina Pronin Manjusha Neerukonda Valentina Curella Sibylle Neufang Sandra Koch Heiko Weichert Hans Weber Andreas Börner Detlef Schuppan Katharina Anne Scherf |
| author_facet | Sabrina Geisslitz Darina Pronin Manjusha Neerukonda Valentina Curella Sibylle Neufang Sandra Koch Heiko Weichert Hans Weber Andreas Börner Detlef Schuppan Katharina Anne Scherf |
| author_sort | Sabrina Geisslitz |
| collection | DOAJ |
| description | Abstract The prevalence of hypersensitivities towards wheat has increased in the last decades. Apart from celiac disease these include allergic and other inflammatory reactions summarized under the term non-celiac wheat sensitivity. One suspected trigger is the family of amylase/trypsin-inhibitors (ATIs), non-gluten proteins that are prominent wheat allergens and that activate the toll-like receptor 4 on intestinal immune cells to promote intestinal and extra-intestinal inflammation. We therefore quantified 13 ATIs in 60 German hexaploid winter wheat cultivars originating from 1891 to 2010 and harvested in three years by targeted liquid chromatography-tandem mass spectrometry combined with stable isotope dilution assay using specific marker peptides as internal standards. The total ATI content and that of the two major ATIs 0.19 and CM3 did not change from old cultivars (first registered from 1891 to 1950) to modern cultivars (1951–2010). There were also no significant changes in ATI distribution. |
| first_indexed | 2024-03-09T14:53:22Z |
| format | Article |
| id | doaj.art-89fae05836894d5cb84085b5b4f35f51 |
| institution | Directory Open Access Journal |
| issn | 2396-8370 |
| language | English |
| last_indexed | 2024-03-09T14:53:22Z |
| publishDate | 2023-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | npj Science of Food |
| spelling | doaj.art-89fae05836894d5cb84085b5b4f35f512023-11-26T14:20:28ZengNature Portfolionpj Science of Food2396-83702023-08-01711810.1038/s41538-023-00219-wBreeding from 1891 to 2010 did not increase the content of amylase/trypsin-inhibitors in wheat (Triticum aestivum)Sabrina Geisslitz0Darina Pronin1Manjusha Neerukonda2Valentina Curella3Sibylle Neufang4Sandra Koch5Heiko Weichert6Hans Weber7Andreas Börner8Detlef Schuppan9Katharina Anne Scherf10Department of Bioactive and Functional Food Chemistry, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT)Leibniz-Institute for Food Systems Biology at the Technical University of MunichInstitute of Translational Immunology and Research Center for Immune Therapy, University Medical CenterInstitute of Translational Immunology and Research Center for Immune Therapy, University Medical CenterInstitute of Translational Immunology and Research Center for Immune Therapy, University Medical CenterInstitute of Translational Immunology and Research Center for Immune Therapy, University Medical CenterDepartment of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant ResearchDepartment of Molecular Genetics, Leibniz Institute of Plant Genetics and Crop Plant ResearchGenebank Department, Leibniz Institute of Plant Genetics and Crop Plant ResearchInstitute of Translational Immunology and Research Center for Immune Therapy, University Medical CenterDepartment of Bioactive and Functional Food Chemistry, Institute of Applied Biosciences, Karlsruhe Institute of Technology (KIT)Abstract The prevalence of hypersensitivities towards wheat has increased in the last decades. Apart from celiac disease these include allergic and other inflammatory reactions summarized under the term non-celiac wheat sensitivity. One suspected trigger is the family of amylase/trypsin-inhibitors (ATIs), non-gluten proteins that are prominent wheat allergens and that activate the toll-like receptor 4 on intestinal immune cells to promote intestinal and extra-intestinal inflammation. We therefore quantified 13 ATIs in 60 German hexaploid winter wheat cultivars originating from 1891 to 2010 and harvested in three years by targeted liquid chromatography-tandem mass spectrometry combined with stable isotope dilution assay using specific marker peptides as internal standards. The total ATI content and that of the two major ATIs 0.19 and CM3 did not change from old cultivars (first registered from 1891 to 1950) to modern cultivars (1951–2010). There were also no significant changes in ATI distribution.https://doi.org/10.1038/s41538-023-00219-w |
| spellingShingle | Sabrina Geisslitz Darina Pronin Manjusha Neerukonda Valentina Curella Sibylle Neufang Sandra Koch Heiko Weichert Hans Weber Andreas Börner Detlef Schuppan Katharina Anne Scherf Breeding from 1891 to 2010 did not increase the content of amylase/trypsin-inhibitors in wheat (Triticum aestivum) npj Science of Food |
| title | Breeding from 1891 to 2010 did not increase the content of amylase/trypsin-inhibitors in wheat (Triticum aestivum) |
| title_full | Breeding from 1891 to 2010 did not increase the content of amylase/trypsin-inhibitors in wheat (Triticum aestivum) |
| title_fullStr | Breeding from 1891 to 2010 did not increase the content of amylase/trypsin-inhibitors in wheat (Triticum aestivum) |
| title_full_unstemmed | Breeding from 1891 to 2010 did not increase the content of amylase/trypsin-inhibitors in wheat (Triticum aestivum) |
| title_short | Breeding from 1891 to 2010 did not increase the content of amylase/trypsin-inhibitors in wheat (Triticum aestivum) |
| title_sort | breeding from 1891 to 2010 did not increase the content of amylase trypsin inhibitors in wheat triticum aestivum |
| url | https://doi.org/10.1038/s41538-023-00219-w |
| work_keys_str_mv | AT sabrinageisslitz breedingfrom1891to2010didnotincreasethecontentofamylasetrypsininhibitorsinwheattriticumaestivum AT darinapronin breedingfrom1891to2010didnotincreasethecontentofamylasetrypsininhibitorsinwheattriticumaestivum AT manjushaneerukonda breedingfrom1891to2010didnotincreasethecontentofamylasetrypsininhibitorsinwheattriticumaestivum AT valentinacurella breedingfrom1891to2010didnotincreasethecontentofamylasetrypsininhibitorsinwheattriticumaestivum AT sibylleneufang breedingfrom1891to2010didnotincreasethecontentofamylasetrypsininhibitorsinwheattriticumaestivum AT sandrakoch breedingfrom1891to2010didnotincreasethecontentofamylasetrypsininhibitorsinwheattriticumaestivum AT heikoweichert breedingfrom1891to2010didnotincreasethecontentofamylasetrypsininhibitorsinwheattriticumaestivum AT hansweber breedingfrom1891to2010didnotincreasethecontentofamylasetrypsininhibitorsinwheattriticumaestivum AT andreasborner breedingfrom1891to2010didnotincreasethecontentofamylasetrypsininhibitorsinwheattriticumaestivum AT detlefschuppan breedingfrom1891to2010didnotincreasethecontentofamylasetrypsininhibitorsinwheattriticumaestivum AT katharinaannescherf breedingfrom1891to2010didnotincreasethecontentofamylasetrypsininhibitorsinwheattriticumaestivum |