Testis-Specific Isoform of Na<sup>+</sup>-K<sup>+</sup> ATPase and Regulation of Bull Fertility
An advanced understanding of sperm function is relevant for evidence-based male fertility prediction and addressing male infertility. A standard breeding soundness evaluation (BSE) merely identifies gross abnormalities in bulls, whereas selection based on single nucleotide polymorphisms and genomic...
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/14/7936 |
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| author | Saurabh Tiwari Gayathri Rajamanickam Veena Unnikrishnan Mina Ojaghi John P. Kastelic Jacob C. Thundathil |
| author_facet | Saurabh Tiwari Gayathri Rajamanickam Veena Unnikrishnan Mina Ojaghi John P. Kastelic Jacob C. Thundathil |
| author_sort | Saurabh Tiwari |
| collection | DOAJ |
| description | An advanced understanding of sperm function is relevant for evidence-based male fertility prediction and addressing male infertility. A standard breeding soundness evaluation (BSE) merely identifies gross abnormalities in bulls, whereas selection based on single nucleotide polymorphisms and genomic estimated breeding values overlooks sub-microscopic differences in sperm. Molecular tools are important for validating genomic selection and advancing knowledge on the regulation of male fertility at an interdisciplinary level. Therefore, research in this field is now focused on developing a combination of in vitro sperm function tests and identifying biomarkers such as sperm proteins with critical roles in fertility. The Na<sup>+</sup>-K<sup>+</sup> ATPase is a ubiquitous transmembrane protein and its α4 isoform (ATP1A4) is exclusively expressed in germ cells and sperm. Furthermore, ATP1A4 is essential for male fertility, as it interacts with signaling molecules in both raft and non-raft fractions of the sperm plasma membrane to regulate capacitation-associated signaling, hyperactivation, sperm-oocyte interactions, and activation. Interestingly, ATP1A4 activity and expression increase during capacitation, challenging the widely accepted dogma of sperm translational quiescence. This review discusses the literature on the role of ATP1A4 during capacitation and fertilization events and its prospective use in improving male fertility prediction. |
| first_indexed | 2024-03-09T13:41:28Z |
| format | Article |
| id | doaj.art-89fbd75aa7a94da5bd8e6e74c5bfbe20 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T13:41:28Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-89fbd75aa7a94da5bd8e6e74c5bfbe202023-11-30T21:06:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012314793610.3390/ijms23147936Testis-Specific Isoform of Na<sup>+</sup>-K<sup>+</sup> ATPase and Regulation of Bull FertilitySaurabh Tiwari0Gayathri Rajamanickam1Veena Unnikrishnan2Mina Ojaghi3John P. Kastelic4Jacob C. Thundathil5Department of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, CanadaDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, CanadaDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, CanadaDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, CanadaDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, CanadaDepartment of Production Animal Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB T2N 4N1, CanadaAn advanced understanding of sperm function is relevant for evidence-based male fertility prediction and addressing male infertility. A standard breeding soundness evaluation (BSE) merely identifies gross abnormalities in bulls, whereas selection based on single nucleotide polymorphisms and genomic estimated breeding values overlooks sub-microscopic differences in sperm. Molecular tools are important for validating genomic selection and advancing knowledge on the regulation of male fertility at an interdisciplinary level. Therefore, research in this field is now focused on developing a combination of in vitro sperm function tests and identifying biomarkers such as sperm proteins with critical roles in fertility. The Na<sup>+</sup>-K<sup>+</sup> ATPase is a ubiquitous transmembrane protein and its α4 isoform (ATP1A4) is exclusively expressed in germ cells and sperm. Furthermore, ATP1A4 is essential for male fertility, as it interacts with signaling molecules in both raft and non-raft fractions of the sperm plasma membrane to regulate capacitation-associated signaling, hyperactivation, sperm-oocyte interactions, and activation. Interestingly, ATP1A4 activity and expression increase during capacitation, challenging the widely accepted dogma of sperm translational quiescence. This review discusses the literature on the role of ATP1A4 during capacitation and fertilization events and its prospective use in improving male fertility prediction.https://www.mdpi.com/1422-0067/23/14/7936ATP1A4capacitationmale fertilitysignalingsperm |
| spellingShingle | Saurabh Tiwari Gayathri Rajamanickam Veena Unnikrishnan Mina Ojaghi John P. Kastelic Jacob C. Thundathil Testis-Specific Isoform of Na<sup>+</sup>-K<sup>+</sup> ATPase and Regulation of Bull Fertility International Journal of Molecular Sciences ATP1A4 capacitation male fertility signaling sperm |
| title | Testis-Specific Isoform of Na<sup>+</sup>-K<sup>+</sup> ATPase and Regulation of Bull Fertility |
| title_full | Testis-Specific Isoform of Na<sup>+</sup>-K<sup>+</sup> ATPase and Regulation of Bull Fertility |
| title_fullStr | Testis-Specific Isoform of Na<sup>+</sup>-K<sup>+</sup> ATPase and Regulation of Bull Fertility |
| title_full_unstemmed | Testis-Specific Isoform of Na<sup>+</sup>-K<sup>+</sup> ATPase and Regulation of Bull Fertility |
| title_short | Testis-Specific Isoform of Na<sup>+</sup>-K<sup>+</sup> ATPase and Regulation of Bull Fertility |
| title_sort | testis specific isoform of na sup sup k sup sup atpase and regulation of bull fertility |
| topic | ATP1A4 capacitation male fertility signaling sperm |
| url | https://www.mdpi.com/1422-0067/23/14/7936 |
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