Cell type boundaries organize plant development
In plants the dorsoventral boundary of leaves defines an axis of symmetry through the centre of the organ separating the top (dorsal) and bottom (ventral) tissues. Although the positioning of this boundary is critical for leaf morphogenesis, how the boundary is established and how it influences deve...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2017-09-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/27421 |
| _version_ | 1811235925606268928 |
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| author | Monica Pia Caggiano Xiulian Yu Neha Bhatia André Larsson Hasthi Ram Carolyn K Ohno Pia Sappl Elliot M Meyerowitz Henrik Jönsson Marcus G Heisler |
| author_facet | Monica Pia Caggiano Xiulian Yu Neha Bhatia André Larsson Hasthi Ram Carolyn K Ohno Pia Sappl Elliot M Meyerowitz Henrik Jönsson Marcus G Heisler |
| author_sort | Monica Pia Caggiano |
| collection | DOAJ |
| description | In plants the dorsoventral boundary of leaves defines an axis of symmetry through the centre of the organ separating the top (dorsal) and bottom (ventral) tissues. Although the positioning of this boundary is critical for leaf morphogenesis, how the boundary is established and how it influences development remains unclear. Using live-imaging and perturbation experiments we show that leaf orientation, morphology and position are pre-patterned by HD-ZIPIII and KAN gene expression in the shoot, leading to a model in which dorsoventral genes coordinate to regulate plant development by localizing auxin response between their expression domains. However we also find that auxin levels feedback on dorsoventral patterning by spatially organizing HD-ZIPIII and KAN expression in the shoot periphery. By demonstrating that the regulation of these genes by auxin also governs their response to wounds, our results also provide a parsimonious explanation for the influence of wounds on leaf dorsoventrality. |
| first_indexed | 2024-04-12T12:00:31Z |
| format | Article |
| id | doaj.art-89fef880344042edb2ea8a974ab30383 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T12:00:31Z |
| publishDate | 2017-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-89fef880344042edb2ea8a974ab303832022-12-22T03:33:52ZengeLife Sciences Publications LtdeLife2050-084X2017-09-01610.7554/eLife.27421Cell type boundaries organize plant developmentMonica Pia Caggiano0Xiulian Yu1Neha Bhatia2https://orcid.org/0000-0002-2165-5183André Larsson3Hasthi Ram4Carolyn K Ohno5Pia Sappl6Elliot M Meyerowitz7https://orcid.org/0000-0003-4798-5153Henrik Jönsson8https://orcid.org/0000-0003-2340-588XMarcus G Heisler9https://orcid.org/0000-0001-5644-8398European Molecular Biology Laboratory, Heidelberg, GermanyEuropean Molecular Biology Laboratory, Heidelberg, GermanyEuropean Molecular Biology Laboratory, Heidelberg, Germany; School of Life and Environmental Sciences, University of Sydney, Sydney, AustraliaComputational Biology and Biological Physics, Department of Astronomy and Theoretical Physics, Lund University, Lund, SwedenEuropean Molecular Biology Laboratory, Heidelberg, GermanyEuropean Molecular Biology Laboratory, Heidelberg, Germany; School of Life and Environmental Sciences, University of Sydney, Sydney, AustraliaEuropean Molecular Biology Laboratory, Heidelberg, GermanyDivision of Biology and Biological Engineering, California Institute of Technology, Howard Hughes Medical Institute, Pasadena, United StatesComputational Biology and Biological Physics, Department of Astronomy and Theoretical Physics, Lund University, Lund, Sweden; Sainsbury Laboratory, University of Cambridge, Cambridge, United Kingdom; Department of Applied Mathematics and Theoretical Physics, University of Cambridge, Cambridge, United KingdomEuropean Molecular Biology Laboratory, Heidelberg, Germany; School of Life and Environmental Sciences, University of Sydney, Sydney, AustraliaIn plants the dorsoventral boundary of leaves defines an axis of symmetry through the centre of the organ separating the top (dorsal) and bottom (ventral) tissues. Although the positioning of this boundary is critical for leaf morphogenesis, how the boundary is established and how it influences development remains unclear. Using live-imaging and perturbation experiments we show that leaf orientation, morphology and position are pre-patterned by HD-ZIPIII and KAN gene expression in the shoot, leading to a model in which dorsoventral genes coordinate to regulate plant development by localizing auxin response between their expression domains. However we also find that auxin levels feedback on dorsoventral patterning by spatially organizing HD-ZIPIII and KAN expression in the shoot periphery. By demonstrating that the regulation of these genes by auxin also governs their response to wounds, our results also provide a parsimonious explanation for the influence of wounds on leaf dorsoventrality.https://elifesciences.org/articles/27421leafmeristemorganogenesisboundariesmorphogenesisauxin |
| spellingShingle | Monica Pia Caggiano Xiulian Yu Neha Bhatia André Larsson Hasthi Ram Carolyn K Ohno Pia Sappl Elliot M Meyerowitz Henrik Jönsson Marcus G Heisler Cell type boundaries organize plant development eLife leaf meristem organogenesis boundaries morphogenesis auxin |
| title | Cell type boundaries organize plant development |
| title_full | Cell type boundaries organize plant development |
| title_fullStr | Cell type boundaries organize plant development |
| title_full_unstemmed | Cell type boundaries organize plant development |
| title_short | Cell type boundaries organize plant development |
| title_sort | cell type boundaries organize plant development |
| topic | leaf meristem organogenesis boundaries morphogenesis auxin |
| url | https://elifesciences.org/articles/27421 |
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