Chitosan nanoparticles loaded with Foeniculum vulgare extract regulate retrieval of sensory and motor functions in mice
In this study, chitosan nanoparticles (CSNPs) encapsulating Foeniculum vulgare (FV) seed extract (SE) were prepared for the controlled delivery of bioactive phytoconstituents. The prepared CSNPs encapsulating FVSE as sustain-releasing nanoconjugate (CSNPs-FVSE) was used as a potent source of functio...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-02-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024014452 |
| _version_ | 1797304489158377472 |
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| author | Majed A. Bajaber Arruje Hameed Ghulam Hussain Razia Noreen Muhammad Ibrahim Shaheera Batool Muhammad Abdul Qayyum Tahir Farooq Bushra Parveen Tanzeela Khalid Perveen Kanwal |
| author_facet | Majed A. Bajaber Arruje Hameed Ghulam Hussain Razia Noreen Muhammad Ibrahim Shaheera Batool Muhammad Abdul Qayyum Tahir Farooq Bushra Parveen Tanzeela Khalid Perveen Kanwal |
| author_sort | Majed A. Bajaber |
| collection | DOAJ |
| description | In this study, chitosan nanoparticles (CSNPs) encapsulating Foeniculum vulgare (FV) seed extract (SE) were prepared for the controlled delivery of bioactive phytoconstituents. The prepared CSNPs encapsulating FVSE as sustain-releasing nanoconjugate (CSNPs-FVSE) was used as a potent source of functional metabolites including kaempferol and quercetin for accelerated reclamation of sensory and motor functions following peripheral nerve injury (PNI). The nanoconjugate exhibited in vitro a biphasic diffusion-controlled sustained release of quercetin and kaempferol ensuring prolonged therapeutic effects. The CSNPs-FVSE was administered through gavaging to albino mice daily at a dose rate of 25 mg/kg body weight from the day of induced PNI till the end of the experiment. The conjugate-treatment induced a significant acceleration in the regain of motor functioning, evaluated from the sciatic function index (SFI) and muscle grip strength studies. Further, the hotplate test confirmed a significantly faster recuperation of sensory functions in conjugate-treated group compared to control. An array of underlying biochemical pathways regulates the regeneration under well-optimized glucose and oxidant levels. Therefore, oxidant status (TOS), blood glycemic level and total antioxidant capacity (TAC) were evaluated in the conjugate-treated group and compared with the controls. The treated subjects exhibited controlled oxidative stress and regulated blood sugars compared to the non-treated control. Thus, the nanoconjugate enriched with polyphenolics significantly accelerated the regeneration and recovery of functions after nerve lesions. The biocompatible nanocarriers encapsulating the nontoxic natural bioactive constitutents have great medicinal and economic value. |
| first_indexed | 2024-03-08T00:10:16Z |
| format | Article |
| id | doaj.art-8a027d733b714736a92535be7400e5c7 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T00:10:16Z |
| publishDate | 2024-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-8a027d733b714736a92535be7400e5c72024-02-17T06:41:04ZengElsevierHeliyon2405-84402024-02-01103e25414Chitosan nanoparticles loaded with Foeniculum vulgare extract regulate retrieval of sensory and motor functions in miceMajed A. Bajaber0Arruje Hameed1Ghulam Hussain2Razia Noreen3Muhammad Ibrahim4Shaheera Batool5Muhammad Abdul Qayyum6Tahir Farooq7Bushra Parveen8Tanzeela Khalid9Perveen Kanwal10Chemistry Department, Faculty of Science, King Khalid University, P.O. Box 9004, Abha, 61413, Saudi ArabiaDepartment of Biochemistry, Government College University Faisalabad, PakistanNeurochemicalbiology and Genetics Laboratory (NGL), Department of Physiology, Government College University Faisalabad, PakistanDepartment of Biochemistry, Government College University Faisalabad, PakistanDepartment of Applied Chemistry, Government College University Faisalabad, PakistanDepartment of Biochemistry, CMH Institute of Medical Sciences Multan, Multan, PakistanDepartment of Chemistry, Division of Science & Technology, University of Education, Lahore, PakistanDepartment of Applied Chemistry, Government College University Faisalabad, Pakistan; Corresponding author.Department of Chemistry, Government College University Faisalabad, PakistanDepartment of Applied Chemistry, Government College University Faisalabad, Pakistan; Corresponding author.Department of Chemistry, The Women University of Multan, Multan, 66000, PakistanIn this study, chitosan nanoparticles (CSNPs) encapsulating Foeniculum vulgare (FV) seed extract (SE) were prepared for the controlled delivery of bioactive phytoconstituents. The prepared CSNPs encapsulating FVSE as sustain-releasing nanoconjugate (CSNPs-FVSE) was used as a potent source of functional metabolites including kaempferol and quercetin for accelerated reclamation of sensory and motor functions following peripheral nerve injury (PNI). The nanoconjugate exhibited in vitro a biphasic diffusion-controlled sustained release of quercetin and kaempferol ensuring prolonged therapeutic effects. The CSNPs-FVSE was administered through gavaging to albino mice daily at a dose rate of 25 mg/kg body weight from the day of induced PNI till the end of the experiment. The conjugate-treatment induced a significant acceleration in the regain of motor functioning, evaluated from the sciatic function index (SFI) and muscle grip strength studies. Further, the hotplate test confirmed a significantly faster recuperation of sensory functions in conjugate-treated group compared to control. An array of underlying biochemical pathways regulates the regeneration under well-optimized glucose and oxidant levels. Therefore, oxidant status (TOS), blood glycemic level and total antioxidant capacity (TAC) were evaluated in the conjugate-treated group and compared with the controls. The treated subjects exhibited controlled oxidative stress and regulated blood sugars compared to the non-treated control. Thus, the nanoconjugate enriched with polyphenolics significantly accelerated the regeneration and recovery of functions after nerve lesions. The biocompatible nanocarriers encapsulating the nontoxic natural bioactive constitutents have great medicinal and economic value.http://www.sciencedirect.com/science/article/pii/S2405844024014452NanoconjugateNerve lesionsAntioxidantsNanocarriersBioavailability |
| spellingShingle | Majed A. Bajaber Arruje Hameed Ghulam Hussain Razia Noreen Muhammad Ibrahim Shaheera Batool Muhammad Abdul Qayyum Tahir Farooq Bushra Parveen Tanzeela Khalid Perveen Kanwal Chitosan nanoparticles loaded with Foeniculum vulgare extract regulate retrieval of sensory and motor functions in mice Heliyon Nanoconjugate Nerve lesions Antioxidants Nanocarriers Bioavailability |
| title | Chitosan nanoparticles loaded with Foeniculum vulgare extract regulate retrieval of sensory and motor functions in mice |
| title_full | Chitosan nanoparticles loaded with Foeniculum vulgare extract regulate retrieval of sensory and motor functions in mice |
| title_fullStr | Chitosan nanoparticles loaded with Foeniculum vulgare extract regulate retrieval of sensory and motor functions in mice |
| title_full_unstemmed | Chitosan nanoparticles loaded with Foeniculum vulgare extract regulate retrieval of sensory and motor functions in mice |
| title_short | Chitosan nanoparticles loaded with Foeniculum vulgare extract regulate retrieval of sensory and motor functions in mice |
| title_sort | chitosan nanoparticles loaded with foeniculum vulgare extract regulate retrieval of sensory and motor functions in mice |
| topic | Nanoconjugate Nerve lesions Antioxidants Nanocarriers Bioavailability |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024014452 |
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