Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic <i>Brassica</i> Allohexaploids
Polyploidy plays a crucial role in plant evolution and speciation. The development of male and female gametes is essential to the reproductive capacity of polyploids, but their gene expression pattern has not been fully explored in newly established polyploids. The present study aimed to reveal a de...
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| Format: | Article |
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MDPI AG
2022-06-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/12/1556 |
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| author | Chengyan Ji Zhaoran Tian Yue Liu Gongyao Shi Baoming Tian Weiwei Chen Zhengqing Xie Xingzhou Han Niannian Liang Fang Wei Xiaochun Wei |
| author_facet | Chengyan Ji Zhaoran Tian Yue Liu Gongyao Shi Baoming Tian Weiwei Chen Zhengqing Xie Xingzhou Han Niannian Liang Fang Wei Xiaochun Wei |
| author_sort | Chengyan Ji |
| collection | DOAJ |
| description | Polyploidy plays a crucial role in plant evolution and speciation. The development of male and female gametes is essential to the reproductive capacity of polyploids, but their gene expression pattern has not been fully explored in newly established polyploids. The present study aimed to reveal a detailed atlas of gene expression for gamete development in newly synthetic <i>Brassica</i> allohexaploids that are not naturally existing species. Comparative transcriptome profiling between developing anthers (staged from meiosis to mature pollen) and ovules (staged from meiosis to mature embryo sac) was performed using RNA-Seq analysis. A total of 8676, 9775 and 4553 upregulated differentially expressed genes (DEGs) were identified for the development of both gametes, for male-only, and for female-only gamete development, respectively, in the synthetic <i>Brassica</i> allohexaploids. By combining gene ontology (GO) biological process analysis and data from the published literature, we identified 37 candidate genes for DNA double-strand break formation, synapsis and the crossover of homologous recombination during male and female meiosis and 51 candidate genes for tapetum development, sporopollenin biosynthesis and pollen wall development in male gamete development. Furthermore, 23 candidate genes for mitotic progression, nuclear positioning and cell specification and development were enriched in female gamete development. This study lays a good foundation for revealing the molecular regulation of genes related to male and female gamete development in <i>Brassica</i> allohexaploids and provides more resourceful genetic information on the reproductive biology of <i>Brassica</i> polyploid breeding. |
| first_indexed | 2024-03-09T22:42:40Z |
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| language | English |
| last_indexed | 2024-03-09T22:42:40Z |
| publishDate | 2022-06-01 |
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| series | Plants |
| spelling | doaj.art-8a05d9f9febb448e81c44f389fb4e0182023-11-23T18:34:31ZengMDPI AGPlants2223-77472022-06-011112155610.3390/plants11121556Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic <i>Brassica</i> AllohexaploidsChengyan Ji0Zhaoran Tian1Yue Liu2Gongyao Shi3Baoming Tian4Weiwei Chen5Zhengqing Xie6Xingzhou Han7Niannian Liang8Fang Wei9Xiaochun Wei10Henan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaHenan International Joint Laboratory of Crop Gene Resources and Improvements, School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450001, ChinaInstitute of Horticulture, Henan Academy of Agricultural Sciences, Graduate T & R Base of Zhengzhou University, Zhengzhou 450002, ChinaPolyploidy plays a crucial role in plant evolution and speciation. The development of male and female gametes is essential to the reproductive capacity of polyploids, but their gene expression pattern has not been fully explored in newly established polyploids. The present study aimed to reveal a detailed atlas of gene expression for gamete development in newly synthetic <i>Brassica</i> allohexaploids that are not naturally existing species. Comparative transcriptome profiling between developing anthers (staged from meiosis to mature pollen) and ovules (staged from meiosis to mature embryo sac) was performed using RNA-Seq analysis. A total of 8676, 9775 and 4553 upregulated differentially expressed genes (DEGs) were identified for the development of both gametes, for male-only, and for female-only gamete development, respectively, in the synthetic <i>Brassica</i> allohexaploids. By combining gene ontology (GO) biological process analysis and data from the published literature, we identified 37 candidate genes for DNA double-strand break formation, synapsis and the crossover of homologous recombination during male and female meiosis and 51 candidate genes for tapetum development, sporopollenin biosynthesis and pollen wall development in male gamete development. Furthermore, 23 candidate genes for mitotic progression, nuclear positioning and cell specification and development were enriched in female gamete development. This study lays a good foundation for revealing the molecular regulation of genes related to male and female gamete development in <i>Brassica</i> allohexaploids and provides more resourceful genetic information on the reproductive biology of <i>Brassica</i> polyploid breeding.https://www.mdpi.com/2223-7747/11/12/1556transcriptome<i>Brassica</i>allohexaploidpolyploidmale and female gamete developmentmeiosis |
| spellingShingle | Chengyan Ji Zhaoran Tian Yue Liu Gongyao Shi Baoming Tian Weiwei Chen Zhengqing Xie Xingzhou Han Niannian Liang Fang Wei Xiaochun Wei Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic <i>Brassica</i> Allohexaploids Plants transcriptome <i>Brassica</i> allohexaploid polyploid male and female gamete development meiosis |
| title | Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic <i>Brassica</i> Allohexaploids |
| title_full | Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic <i>Brassica</i> Allohexaploids |
| title_fullStr | Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic <i>Brassica</i> Allohexaploids |
| title_full_unstemmed | Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic <i>Brassica</i> Allohexaploids |
| title_short | Transcriptome Profiling Identifies Candidate Genes Contributing to Male and Female Gamete Development in Synthetic <i>Brassica</i> Allohexaploids |
| title_sort | transcriptome profiling identifies candidate genes contributing to male and female gamete development in synthetic i brassica i allohexaploids |
| topic | transcriptome <i>Brassica</i> allohexaploid polyploid male and female gamete development meiosis |
| url | https://www.mdpi.com/2223-7747/11/12/1556 |
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