Highly efficient system for the micropropagation and acclimatization of pineapple in vitro plants (Ananas comosus L. Merr, var. MD2)
The low acclimatization efficiency of pineapple seedlings obtained by in vitro culture has been one of the main limitations to massively scale micropropagation protocols. Various factors may be affecting the successful establishment of in vitro plants to ex vitro conditions, related to the plant an...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Julius Kühn-Institut
2023-10-01
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| Series: | Journal of Applied Botany and Food Quality |
| Online Access: | https://ojs.openagrar.de/index.php/JABFQ/article/view/17032 |
| _version_ | 1797665883247607808 |
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| author | Adolfo Guzmán Antonio Adriana Canto Flick Susana Avilés Viñas Yaritza Rodriguez Llanes Gema Pijeira Fernández Nancy Santana Buzzy |
| author_facet | Adolfo Guzmán Antonio Adriana Canto Flick Susana Avilés Viñas Yaritza Rodriguez Llanes Gema Pijeira Fernández Nancy Santana Buzzy |
| author_sort | Adolfo Guzmán Antonio |
| collection | DOAJ |
| description |
The low acclimatization efficiency of pineapple seedlings obtained by in vitro culture has been one of the main limitations to massively scale micropropagation protocols. Various factors may be affecting the successful establishment of in vitro plants to ex vitro conditions, related to the plant and/or the environment and the management of seedlings. The objective of this study was to establish an efficient micropropagation and acclimatization system for the MD2 variety, with a high in vitro multiplication rate and a high greenhouse and field survival of pineapple in vitro plants. In the multiplication phase, the axillary buds were isolated and placed on a semisolid medium and three induction treatments with different concentrations of BAP (1.0, 3.0, 5.0 mg/L) and ANA (2.0 mg/L), were evaluated. After the third subculture, multiple shoots were transferred to a temporary immersion system (BIOMINT). During acclimatization and nursery phases, six treatments with different substrate compositions wereevaluated. The induction treatment IT3 (BAP 5.0 mg/L and ANA 2.0 mg/L) was selected for a significantly high multiplication rate. AT4 treatment (Soil + Coconut fiber + perlite 1:1:1) showed the highest survival rate (95%) and allowed the obtaining of well-developed plants. This system constitutes a valuable technology to introduce in vitro plants to the pineapple production scheme on a commercial scale.
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| first_indexed | 2024-03-11T19:51:26Z |
| format | Article |
| id | doaj.art-8a0618a588184ff69b50d2df89b163a7 |
| institution | Directory Open Access Journal |
| issn | 1613-9216 1439-040X |
| language | English |
| last_indexed | 2024-03-11T19:51:26Z |
| publishDate | 2023-10-01 |
| publisher | Julius Kühn-Institut |
| record_format | Article |
| series | Journal of Applied Botany and Food Quality |
| spelling | doaj.art-8a0618a588184ff69b50d2df89b163a72023-10-05T08:44:03ZengJulius Kühn-InstitutJournal of Applied Botany and Food Quality1613-92161439-040X2023-10-019610.5073/JABFQ.2023.096.014Highly efficient system for the micropropagation and acclimatization of pineapple in vitro plants (Ananas comosus L. Merr, var. MD2)Adolfo Guzmán Antonio0Adriana Canto Flick1Susana Avilés Viñas2Yaritza Rodriguez Llanes3Gema Pijeira Fernández4Nancy Santana Buzzy5Unidad de Bioquímica y Bilogía Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), MéxicoUnidad de Bioquímica y Bilogía Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), MéxicoUnidad de Bioquímica y Bilogía Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), MéxicoUnidad de Bioquímica y Bilogía Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), MéxicoUnidad de Bioquímica y Bilogía Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), MéxicoUnidad de Bioquímica y Bilogía Molecular de Plantas, Centro de Investigación Científica de Yucatán (CICY), México The low acclimatization efficiency of pineapple seedlings obtained by in vitro culture has been one of the main limitations to massively scale micropropagation protocols. Various factors may be affecting the successful establishment of in vitro plants to ex vitro conditions, related to the plant and/or the environment and the management of seedlings. The objective of this study was to establish an efficient micropropagation and acclimatization system for the MD2 variety, with a high in vitro multiplication rate and a high greenhouse and field survival of pineapple in vitro plants. In the multiplication phase, the axillary buds were isolated and placed on a semisolid medium and three induction treatments with different concentrations of BAP (1.0, 3.0, 5.0 mg/L) and ANA (2.0 mg/L), were evaluated. After the third subculture, multiple shoots were transferred to a temporary immersion system (BIOMINT). During acclimatization and nursery phases, six treatments with different substrate compositions wereevaluated. The induction treatment IT3 (BAP 5.0 mg/L and ANA 2.0 mg/L) was selected for a significantly high multiplication rate. AT4 treatment (Soil + Coconut fiber + perlite 1:1:1) showed the highest survival rate (95%) and allowed the obtaining of well-developed plants. This system constitutes a valuable technology to introduce in vitro plants to the pineapple production scheme on a commercial scale. https://ojs.openagrar.de/index.php/JABFQ/article/view/17032 |
| spellingShingle | Adolfo Guzmán Antonio Adriana Canto Flick Susana Avilés Viñas Yaritza Rodriguez Llanes Gema Pijeira Fernández Nancy Santana Buzzy Highly efficient system for the micropropagation and acclimatization of pineapple in vitro plants (Ananas comosus L. Merr, var. MD2) Journal of Applied Botany and Food Quality |
| title | Highly efficient system for the micropropagation and acclimatization of pineapple in vitro plants (Ananas comosus L. Merr, var. MD2) |
| title_full | Highly efficient system for the micropropagation and acclimatization of pineapple in vitro plants (Ananas comosus L. Merr, var. MD2) |
| title_fullStr | Highly efficient system for the micropropagation and acclimatization of pineapple in vitro plants (Ananas comosus L. Merr, var. MD2) |
| title_full_unstemmed | Highly efficient system for the micropropagation and acclimatization of pineapple in vitro plants (Ananas comosus L. Merr, var. MD2) |
| title_short | Highly efficient system for the micropropagation and acclimatization of pineapple in vitro plants (Ananas comosus L. Merr, var. MD2) |
| title_sort | highly efficient system for the micropropagation and acclimatization of pineapple in vitro plants ananas comosus l merr var md2 |
| url | https://ojs.openagrar.de/index.php/JABFQ/article/view/17032 |
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