TEC Expresses Highly in Hepatocellular Carcinoma and Is Involved in Drug Resistance
Objective To investigate the expression level of TEC, a no-receptor tyrosine kinase, in hepatocellular carcinoma(HCC) tissues and its correlation with drug resistance. Methods The expressions of TEC mRNA and protein in carcinoma tissues, paracancerous tissues and HepG2 cell line were determined by R...
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| Format: | Article |
| Language: | zho |
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Magazine House of Cancer Research on Prevention and Treatment
2018-09-01
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| Series: | Zhongliu Fangzhi Yanjiu |
| Subjects: | |
| Online Access: | http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0107.htm |
| _version_ | 1818907668588265472 |
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| author | LIU Wen LI Feifei ZHONG Minggui LIU Yakun FANG Haoshu WANG Siying |
| author_facet | LIU Wen LI Feifei ZHONG Minggui LIU Yakun FANG Haoshu WANG Siying |
| author_sort | LIU Wen |
| collection | DOAJ |
| description | Objective To investigate the expression level of TEC, a no-receptor tyrosine kinase, in hepatocellular carcinoma(HCC) tissues and its correlation with drug resistance. Methods The expressions of TEC mRNA and protein in carcinoma tissues, paracancerous tissues and HepG2 cell line were determined by RT-PCR, Western blot, immunohistochemistrical staining and slot blotting. MTT assay was used to detect the activity of HepG2 cells. A eukaryotic expression vector carrying TEC gene was constructed to obtain high expression level of TEC in HCC, and RNA interference was applied to knock down TEC. Phospho-TEC was also analyzed by CO-IP to determine TEC activation. Results TEC mRNA and protein were both increased significantly in 18 cases of HCC tissues as compared with those in paracancerous tissues(P < 0.001). The high expression of TEC was associated with the increased resistance of 48 cases of HCC tissues to chemotherapeutic drugs; the upregulation of TEC increased the resistance of HepG2 cells to chemotherapeutic drugs. TEC were augmented by the treatment of chemotherapeutics, accompanying with an increase of phospho-ERK. Conclusion TEC is highly expressed in HCC tissues, which is responsible for the drug resistance of HCC and likely related to increased phospho-ERK. |
| first_indexed | 2024-12-19T21:58:47Z |
| format | Article |
| id | doaj.art-8a0619921f524222974108a800284a59 |
| institution | Directory Open Access Journal |
| issn | 1000-8578 1000-8578 |
| language | zho |
| last_indexed | 2024-12-19T21:58:47Z |
| publishDate | 2018-09-01 |
| publisher | Magazine House of Cancer Research on Prevention and Treatment |
| record_format | Article |
| series | Zhongliu Fangzhi Yanjiu |
| spelling | doaj.art-8a0619921f524222974108a800284a592022-12-21T20:04:12ZzhoMagazine House of Cancer Research on Prevention and TreatmentZhongliu Fangzhi Yanjiu1000-85781000-85782018-09-0145966066510.3971/j.issn.1000-8578.2018.18.01078578.2018.18.0107TEC Expresses Highly in Hepatocellular Carcinoma and Is Involved in Drug ResistanceLIU Wen0LI Feifei1ZHONG Minggui2LIU Yakun3FANG Haoshu4WANG Siying5Staff Room of Pathology, Department of Elementary Teaching, Anhui College of Traditional Chinese Medicine, Wuhu 241000, ChinaPathophysiology Department, School of Basic Medical Science, Anhui Medical University, Hefei 230032, ChinaPathophysiology Department, School of Basic Medical Science, Anhui Medical University, Hefei 230032, ChinaPathophysiology Department, School of Basic Medical Science, Anhui Medical University, Hefei 230032, ChinaPathophysiology Department, School of Basic Medical Science, Anhui Medical University, Hefei 230032, ChinaPathophysiology Department, School of Basic Medical Science, Anhui Medical University, Hefei 230032, ChinaObjective To investigate the expression level of TEC, a no-receptor tyrosine kinase, in hepatocellular carcinoma(HCC) tissues and its correlation with drug resistance. Methods The expressions of TEC mRNA and protein in carcinoma tissues, paracancerous tissues and HepG2 cell line were determined by RT-PCR, Western blot, immunohistochemistrical staining and slot blotting. MTT assay was used to detect the activity of HepG2 cells. A eukaryotic expression vector carrying TEC gene was constructed to obtain high expression level of TEC in HCC, and RNA interference was applied to knock down TEC. Phospho-TEC was also analyzed by CO-IP to determine TEC activation. Results TEC mRNA and protein were both increased significantly in 18 cases of HCC tissues as compared with those in paracancerous tissues(P < 0.001). The high expression of TEC was associated with the increased resistance of 48 cases of HCC tissues to chemotherapeutic drugs; the upregulation of TEC increased the resistance of HepG2 cells to chemotherapeutic drugs. TEC were augmented by the treatment of chemotherapeutics, accompanying with an increase of phospho-ERK. Conclusion TEC is highly expressed in HCC tissues, which is responsible for the drug resistance of HCC and likely related to increased phospho-ERK.http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0107.htmhepatocellular carcinomatumor drug resistanceprotein kinasetec |
| spellingShingle | LIU Wen LI Feifei ZHONG Minggui LIU Yakun FANG Haoshu WANG Siying TEC Expresses Highly in Hepatocellular Carcinoma and Is Involved in Drug Resistance Zhongliu Fangzhi Yanjiu hepatocellular carcinoma tumor drug resistance protein kinase tec |
| title | TEC Expresses Highly in Hepatocellular Carcinoma and Is Involved in Drug Resistance |
| title_full | TEC Expresses Highly in Hepatocellular Carcinoma and Is Involved in Drug Resistance |
| title_fullStr | TEC Expresses Highly in Hepatocellular Carcinoma and Is Involved in Drug Resistance |
| title_full_unstemmed | TEC Expresses Highly in Hepatocellular Carcinoma and Is Involved in Drug Resistance |
| title_short | TEC Expresses Highly in Hepatocellular Carcinoma and Is Involved in Drug Resistance |
| title_sort | tec expresses highly in hepatocellular carcinoma and is involved in drug resistance |
| topic | hepatocellular carcinoma tumor drug resistance protein kinase tec |
| url | http://html.rhhz.net/ZLFZYJ/html/8578.2018.18.0107.htm |
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