The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber
Hydrogen gas (H<sub>2</sub>) and glucose (Glc) have been reported as novel antioxidants and signal molecules involved in multiple biological processes in plants. However, the physiological roles and relationships of H<sub>2</sub> and Glc in adventitious rooting are less clear...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/9/1937 |
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| author | Zongxi Zhao Changxia Li Huwei Liu Jingjing Yang Panpan Huang Weibiao Liao |
| author_facet | Zongxi Zhao Changxia Li Huwei Liu Jingjing Yang Panpan Huang Weibiao Liao |
| author_sort | Zongxi Zhao |
| collection | DOAJ |
| description | Hydrogen gas (H<sub>2</sub>) and glucose (Glc) have been reported as novel antioxidants and signal molecules involved in multiple biological processes in plants. However, the physiological roles and relationships of H<sub>2</sub> and Glc in adventitious rooting are less clear. Here, we showed that the effects of different concentrations Glc (0, 0.01, 0.05, 0.10, 0.50 and 1.00 mM) on adventitious rooting in cucumber were dose-dependent, with a maximal biological response at 0.10 mM. While, the positive roles of hydrogen rich water (HRW, a H<sub>2</sub> donor)-regulated adventitious rooting were blocked by a specific Glc inhibitor glucosamine (GlcN), suggesting that Glc might be responsible for H<sub>2</sub>-regulated adventitious root development. HRW increased glucose, sucrose, starch and total sugar contents. Glucose-6-phosphate (G6P), fructose-6-phosphate (F6P) and glucose-1-phosphate (G1P) contents were also increased by HRW. Meanwhile, the activities of sucrose-related enzymes incorporating sucrose synthase (SS) and sucrose phosphate synthase (SPS) and glucose-related enzymes including hexokinase (HK), pyruvate kinase (PK) and adenosine 5′-diphosphate pyrophosphorylase (AGPase) were increased by HRW. Moreover, HRW upregulated the expression levels of sucrose or glucose metabolism-related genes including <i>CsSuSy1</i>, <i>CsSuSy6</i>, <i>CsHK1</i>, <i>CsHK3</i>, <i>CsUDP1</i>, <i>CsUDP1-like</i>, <i>CsG6P1</i> and <i>CsG6P1-like</i>. However, these positive roles were all inhibited by GlcN. Together, H<sub>2</sub> might regulate adventitious rooting by promoting glucose metabolism. |
| first_indexed | 2024-03-10T07:17:29Z |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T07:17:29Z |
| publishDate | 2021-09-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-8a08dcbdddfb424b98b218bbb5f2ecef2023-11-22T14:54:02ZengMDPI AGPlants2223-77472021-09-01109193710.3390/plants10091937The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in CucumberZongxi Zhao0Changxia Li1Huwei Liu2Jingjing Yang3Panpan Huang4Weibiao Liao5College of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, 1 Yinmen Village, Anning District, Lanzhou 730070, ChinaHydrogen gas (H<sub>2</sub>) and glucose (Glc) have been reported as novel antioxidants and signal molecules involved in multiple biological processes in plants. However, the physiological roles and relationships of H<sub>2</sub> and Glc in adventitious rooting are less clear. Here, we showed that the effects of different concentrations Glc (0, 0.01, 0.05, 0.10, 0.50 and 1.00 mM) on adventitious rooting in cucumber were dose-dependent, with a maximal biological response at 0.10 mM. While, the positive roles of hydrogen rich water (HRW, a H<sub>2</sub> donor)-regulated adventitious rooting were blocked by a specific Glc inhibitor glucosamine (GlcN), suggesting that Glc might be responsible for H<sub>2</sub>-regulated adventitious root development. HRW increased glucose, sucrose, starch and total sugar contents. Glucose-6-phosphate (G6P), fructose-6-phosphate (F6P) and glucose-1-phosphate (G1P) contents were also increased by HRW. Meanwhile, the activities of sucrose-related enzymes incorporating sucrose synthase (SS) and sucrose phosphate synthase (SPS) and glucose-related enzymes including hexokinase (HK), pyruvate kinase (PK) and adenosine 5′-diphosphate pyrophosphorylase (AGPase) were increased by HRW. Moreover, HRW upregulated the expression levels of sucrose or glucose metabolism-related genes including <i>CsSuSy1</i>, <i>CsSuSy6</i>, <i>CsHK1</i>, <i>CsHK3</i>, <i>CsUDP1</i>, <i>CsUDP1-like</i>, <i>CsG6P1</i> and <i>CsG6P1-like</i>. However, these positive roles were all inhibited by GlcN. Together, H<sub>2</sub> might regulate adventitious rooting by promoting glucose metabolism.https://www.mdpi.com/2223-7747/10/9/1937glucosaminesucrosestarchgene expressionsugar metabolism |
| spellingShingle | Zongxi Zhao Changxia Li Huwei Liu Jingjing Yang Panpan Huang Weibiao Liao The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber Plants glucosamine sucrose starch gene expression sugar metabolism |
| title | The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber |
| title_full | The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber |
| title_fullStr | The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber |
| title_full_unstemmed | The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber |
| title_short | The Involvement of Glucose in Hydrogen Gas-Medicated Adventitious Rooting in Cucumber |
| title_sort | involvement of glucose in hydrogen gas medicated adventitious rooting in cucumber |
| topic | glucosamine sucrose starch gene expression sugar metabolism |
| url | https://www.mdpi.com/2223-7747/10/9/1937 |
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