<i>Mlig-SKP1</i> Gene Is Required for Spermatogenesis in the Flatworm <i>Macrostomum lignano</i>
In a free-living flatworm, <i>Macrostomum lignano</i>, an S-phase kinase-associated protein 1 (<i>SKP1</i>) homologous gene was identified as enriched in proliferating cells, suggesting that it can function in the regulation of stem cells or germline cells since these are the...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/23/15110 |
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| author | Mikhail Biryukov Anastasia Dmitrieva Valeriya Vavilova Kirill Ustyantsev Erzhena Bazarova Igor Sukhikh Eugene Berezikov Alexandr Blinov |
| author_facet | Mikhail Biryukov Anastasia Dmitrieva Valeriya Vavilova Kirill Ustyantsev Erzhena Bazarova Igor Sukhikh Eugene Berezikov Alexandr Blinov |
| author_sort | Mikhail Biryukov |
| collection | DOAJ |
| description | In a free-living flatworm, <i>Macrostomum lignano</i>, an S-phase kinase-associated protein 1 (<i>SKP1</i>) homologous gene was identified as enriched in proliferating cells, suggesting that it can function in the regulation of stem cells or germline cells since these are the only two types of proliferating cells in flatworms. <i>SKP1</i> is a conserved protein that plays a role in ubiquitination processes as a part of the Skp1-Cullin 1-F-box (SCF) ubiquitin ligase complex. However, the exact role of <i>Mlig-SKP1</i> in <i>M. lignano</i> was not established. Here, we demonstrate that <i>Mlig-SKP1</i> is neither involved in stem cell regulation during homeostasis, nor in regeneration, but is required for spermatogenesis. <i>Mlig-SKP1</i>(RNAi) animals have increased testes size and decreased fertility as a result of the aberrant maturation of sperm cells. Our findings reinforce the role of ubiquitination pathways in germ cell regulation and demonstrate the conserved role of <i>SKP1</i> in spermatogenesis. |
| first_indexed | 2024-03-09T17:45:36Z |
| format | Article |
| id | doaj.art-8a0960afc817412c90550aa82d2b82ef |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T17:45:36Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8a0960afc817412c90550aa82d2b82ef2023-11-24T11:13:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0123231511010.3390/ijms232315110<i>Mlig-SKP1</i> Gene Is Required for Spermatogenesis in the Flatworm <i>Macrostomum lignano</i>Mikhail Biryukov0Anastasia Dmitrieva1Valeriya Vavilova2Kirill Ustyantsev3Erzhena Bazarova4Igor Sukhikh5Eugene Berezikov6Alexandr Blinov7Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Science, 630090 Novosibirsk, RussiaInstitute of Cytology and Genetics, Siberian Branch of Russian Academy of Science, 630090 Novosibirsk, RussiaInstitute of Cytology and Genetics, Siberian Branch of Russian Academy of Science, 630090 Novosibirsk, RussiaEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, 9700AD Groningen, The NetherlandsInstitute of Cytology and Genetics, Siberian Branch of Russian Academy of Science, 630090 Novosibirsk, RussiaInstitute of Cytology and Genetics, Siberian Branch of Russian Academy of Science, 630090 Novosibirsk, RussiaEuropean Research Institute for the Biology of Ageing, University of Groningen, University Medical Center Groningen, 9700AD Groningen, The NetherlandsInstitute of Cytology and Genetics, Siberian Branch of Russian Academy of Science, 630090 Novosibirsk, RussiaIn a free-living flatworm, <i>Macrostomum lignano</i>, an S-phase kinase-associated protein 1 (<i>SKP1</i>) homologous gene was identified as enriched in proliferating cells, suggesting that it can function in the regulation of stem cells or germline cells since these are the only two types of proliferating cells in flatworms. <i>SKP1</i> is a conserved protein that plays a role in ubiquitination processes as a part of the Skp1-Cullin 1-F-box (SCF) ubiquitin ligase complex. However, the exact role of <i>Mlig-SKP1</i> in <i>M. lignano</i> was not established. Here, we demonstrate that <i>Mlig-SKP1</i> is neither involved in stem cell regulation during homeostasis, nor in regeneration, but is required for spermatogenesis. <i>Mlig-SKP1</i>(RNAi) animals have increased testes size and decreased fertility as a result of the aberrant maturation of sperm cells. Our findings reinforce the role of ubiquitination pathways in germ cell regulation and demonstrate the conserved role of <i>SKP1</i> in spermatogenesis.https://www.mdpi.com/1422-0067/23/23/15110spermatogenesisregenerationproliferationstem cellsflatwormsRNA interference |
| spellingShingle | Mikhail Biryukov Anastasia Dmitrieva Valeriya Vavilova Kirill Ustyantsev Erzhena Bazarova Igor Sukhikh Eugene Berezikov Alexandr Blinov <i>Mlig-SKP1</i> Gene Is Required for Spermatogenesis in the Flatworm <i>Macrostomum lignano</i> International Journal of Molecular Sciences spermatogenesis regeneration proliferation stem cells flatworms RNA interference |
| title | <i>Mlig-SKP1</i> Gene Is Required for Spermatogenesis in the Flatworm <i>Macrostomum lignano</i> |
| title_full | <i>Mlig-SKP1</i> Gene Is Required for Spermatogenesis in the Flatworm <i>Macrostomum lignano</i> |
| title_fullStr | <i>Mlig-SKP1</i> Gene Is Required for Spermatogenesis in the Flatworm <i>Macrostomum lignano</i> |
| title_full_unstemmed | <i>Mlig-SKP1</i> Gene Is Required for Spermatogenesis in the Flatworm <i>Macrostomum lignano</i> |
| title_short | <i>Mlig-SKP1</i> Gene Is Required for Spermatogenesis in the Flatworm <i>Macrostomum lignano</i> |
| title_sort | i mlig skp1 i gene is required for spermatogenesis in the flatworm i macrostomum lignano i |
| topic | spermatogenesis regeneration proliferation stem cells flatworms RNA interference |
| url | https://www.mdpi.com/1422-0067/23/23/15110 |
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