Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis
Francisella tularensis secretes tubular outer membrane vesicles (OMVs) that contain a number of immunoreactive proteins as well as virulence factors. We have reported previously that isolated Francisella OMVs enter macrophages, cumulate inside, and induce a strong pro-inflammatory response. In the c...
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| Format: | Artikel |
| Sprache: | English |
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Frontiers Media S.A.
2024-03-01
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| Schriftenreihe: | Frontiers in Microbiology |
| Schlagworte: | |
| Online Zugang: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1355872/full |
| _version_ | 1827320394082680832 |
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| author | Ivona Pavkova Jan Bavlovic Klara Kubelkova Jiri Stulik Jana Klimentova |
| author_facet | Ivona Pavkova Jan Bavlovic Klara Kubelkova Jiri Stulik Jana Klimentova |
| author_sort | Ivona Pavkova |
| collection | DOAJ |
| description | Francisella tularensis secretes tubular outer membrane vesicles (OMVs) that contain a number of immunoreactive proteins as well as virulence factors. We have reported previously that isolated Francisella OMVs enter macrophages, cumulate inside, and induce a strong pro-inflammatory response. In the current article, we present that OMVs treatment of macrophages also enhances phagocytosis of the bacteria and suppresses their intracellular replication. On the other hand, the subsequent infection with Francisella is able to revert to some extent the strong pro-inflammatory effect induced by OMVs in macrophages. Being derived from the bacterial surface, isolated OMVs may be considered a “non-viable mixture of Francisella antigens” and as such, they present a promising protective material. Immunization of mice with OMVs isolated from a virulent F. tularensis subsp. holarctica strain FSC200 prolonged the survival time but did not fully protect against the infection with a lethal dose of the parent strain. However, the sera of the immunized animals revealed unambiguous cytokine and antibody responses and proved to recognize a set of well-known Francisella immunoreactive proteins. For these reasons, Francisella OMVs present an interesting material for future protective studies. |
| first_indexed | 2024-04-25T00:45:14Z |
| format | Article |
| id | doaj.art-8a0d0b92c9ad4268aac1449e0f505967 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-25T00:45:14Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-8a0d0b92c9ad4268aac1449e0f5059672024-03-12T04:55:14ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-03-011510.3389/fmicb.2024.13558721355872Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensisIvona PavkovaJan BavlovicKlara KubelkovaJiri StulikJana KlimentovaFrancisella tularensis secretes tubular outer membrane vesicles (OMVs) that contain a number of immunoreactive proteins as well as virulence factors. We have reported previously that isolated Francisella OMVs enter macrophages, cumulate inside, and induce a strong pro-inflammatory response. In the current article, we present that OMVs treatment of macrophages also enhances phagocytosis of the bacteria and suppresses their intracellular replication. On the other hand, the subsequent infection with Francisella is able to revert to some extent the strong pro-inflammatory effect induced by OMVs in macrophages. Being derived from the bacterial surface, isolated OMVs may be considered a “non-viable mixture of Francisella antigens” and as such, they present a promising protective material. Immunization of mice with OMVs isolated from a virulent F. tularensis subsp. holarctica strain FSC200 prolonged the survival time but did not fully protect against the infection with a lethal dose of the parent strain. However, the sera of the immunized animals revealed unambiguous cytokine and antibody responses and proved to recognize a set of well-known Francisella immunoreactive proteins. For these reasons, Francisella OMVs present an interesting material for future protective studies.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1355872/fullFrancisella tularensisFSC200outer membrane vesicleshost-pathogen interactionvaccination |
| spellingShingle | Ivona Pavkova Jan Bavlovic Klara Kubelkova Jiri Stulik Jana Klimentova Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis Frontiers in Microbiology Francisella tularensis FSC200 outer membrane vesicles host-pathogen interaction vaccination |
| title | Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis |
| title_full | Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis |
| title_fullStr | Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis |
| title_full_unstemmed | Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis |
| title_short | Protective potential of outer membrane vesicles derived from a virulent strain of Francisella tularensis |
| title_sort | protective potential of outer membrane vesicles derived from a virulent strain of francisella tularensis |
| topic | Francisella tularensis FSC200 outer membrane vesicles host-pathogen interaction vaccination |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1355872/full |
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