Effects of nitrite exposure on haematological parameters and growth in clown knifefish (Chitala ornata, Gray 1831)
Physiological responses and growth of clown knifefish (Chitala ornata) (initial weight of 11-12 g) exposed to nitrite were investigated with three separate experiments. The first experiment was to determine the nitrite concentration causing 50% mortality in 96 hours (96-h LC50). The second experimen...
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| Format: | Article |
| Language: | English |
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CAN THO UNIVERSITY PUBLISHING HOUSE
2018-03-01
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| Series: | Can Tho University Journal of Science |
| Subjects: | |
| Online Access: | https://ctujs.ctu.edu.vn/index.php/ctujs/article/view/104 |
| _version_ | 1798039660237160448 |
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| author | Thi Hong Gam Le Thanh Phuong Nguyen Thi Thuy Vu Nguyen Thi Thanh Huong Do Ngoc Nhu Pham |
| author_facet | Thi Hong Gam Le Thanh Phuong Nguyen Thi Thuy Vu Nguyen Thi Thanh Huong Do Ngoc Nhu Pham |
| author_sort | Thi Hong Gam Le |
| collection | DOAJ |
| description | Physiological responses and growth of clown knifefish (Chitala ornata) (initial weight of 11-12 g) exposed to nitrite were investigated with three separate experiments. The first experiment was to determine the nitrite concentration causing 50% mortality in 96 hours (96-h LC50). The second experiment examined the effects of different nitrite concentrations on haematological parameters. The third experiment investigated into the effects of different nitrite concentrations on fish growth including 4 treatments such as control, 0.2 mM nitrite, 0.4 mM nitrite, and 4 mM nitrite for measuring growth parameters at days 0, 30, 60, and 90 days (sampling 30 fish/tank). The results showed that the clown knifefish had high nitrite tolerance with 96-h LC50 of 7.82 mM. There were significant increases in methaemoglobin and leukocytes while other haematological parameters decreased during nitrite exposures at the treatment of 4 mM nitrite. Particularly, methaemoglobin and the number of leukocytes increased from 0.4 to 29.5%, and from 39.89x103 to 72.33x103 cells/mm3, respectively. Differently, there were significant declines in the number of erythrocyte (3.19x106–2.33x106 cells/mm3), haemoglobin (10.47-7.04 mM), and haematocrit (38.07-26.5%) at the highest nitrite treatment. After 90 days, daily weight gain (0.25±0.02 g/day), specific growth rate (1.18±0.07 %/day), survival rate (59%) at the treatment of 4 mM nitrite were significantly lower than those of the control, but no significant difference was observed in such parameters between the control and the treatments of 0.2 or 0.4 mM nitrite. |
| first_indexed | 2024-04-11T21:56:54Z |
| format | Article |
| id | doaj.art-8a1041a4f18e4b0e8e55af00f4a0f788 |
| institution | Directory Open Access Journal |
| issn | 2615-9422 2815-5602 |
| language | English |
| last_indexed | 2024-04-11T21:56:54Z |
| publishDate | 2018-03-01 |
| publisher | CAN THO UNIVERSITY PUBLISHING HOUSE |
| record_format | Article |
| series | Can Tho University Journal of Science |
| spelling | doaj.art-8a1041a4f18e4b0e8e55af00f4a0f7882022-12-22T04:01:04ZengCAN THO UNIVERSITY PUBLISHING HOUSECan Tho University Journal of Science2615-94222815-56022018-03-0154210.22144/ctu.jen.2018.001Effects of nitrite exposure on haematological parameters and growth in clown knifefish (Chitala ornata, Gray 1831)Thi Hong Gam Le0Thanh Phuong Nguyen1Thi Thuy Vu Nguyen2Thi Thanh Huong Do3Ngoc Nhu PhamCollege of Aquaculture and Fisheries, Can Tho UniversityBM.Ky thuat nuoi hai san, Khoa Thủy sảnAgricultural extension center, Ben Tre province, Viet NamBM.Thuy sinh hoc ung dung, Khoa Thủy sảnPhysiological responses and growth of clown knifefish (Chitala ornata) (initial weight of 11-12 g) exposed to nitrite were investigated with three separate experiments. The first experiment was to determine the nitrite concentration causing 50% mortality in 96 hours (96-h LC50). The second experiment examined the effects of different nitrite concentrations on haematological parameters. The third experiment investigated into the effects of different nitrite concentrations on fish growth including 4 treatments such as control, 0.2 mM nitrite, 0.4 mM nitrite, and 4 mM nitrite for measuring growth parameters at days 0, 30, 60, and 90 days (sampling 30 fish/tank). The results showed that the clown knifefish had high nitrite tolerance with 96-h LC50 of 7.82 mM. There were significant increases in methaemoglobin and leukocytes while other haematological parameters decreased during nitrite exposures at the treatment of 4 mM nitrite. Particularly, methaemoglobin and the number of leukocytes increased from 0.4 to 29.5%, and from 39.89x103 to 72.33x103 cells/mm3, respectively. Differently, there were significant declines in the number of erythrocyte (3.19x106–2.33x106 cells/mm3), haemoglobin (10.47-7.04 mM), and haematocrit (38.07-26.5%) at the highest nitrite treatment. After 90 days, daily weight gain (0.25±0.02 g/day), specific growth rate (1.18±0.07 %/day), survival rate (59%) at the treatment of 4 mM nitrite were significantly lower than those of the control, but no significant difference was observed in such parameters between the control and the treatments of 0.2 or 0.4 mM nitrite.https://ctujs.ctu.edu.vn/index.php/ctujs/article/view/104Clown knifefishgrowthhaematological parametersnitrite |
| spellingShingle | Thi Hong Gam Le Thanh Phuong Nguyen Thi Thuy Vu Nguyen Thi Thanh Huong Do Ngoc Nhu Pham Effects of nitrite exposure on haematological parameters and growth in clown knifefish (Chitala ornata, Gray 1831) Can Tho University Journal of Science Clown knifefish growth haematological parameters nitrite |
| title | Effects of nitrite exposure on haematological parameters and growth in clown knifefish (Chitala ornata, Gray 1831) |
| title_full | Effects of nitrite exposure on haematological parameters and growth in clown knifefish (Chitala ornata, Gray 1831) |
| title_fullStr | Effects of nitrite exposure on haematological parameters and growth in clown knifefish (Chitala ornata, Gray 1831) |
| title_full_unstemmed | Effects of nitrite exposure on haematological parameters and growth in clown knifefish (Chitala ornata, Gray 1831) |
| title_short | Effects of nitrite exposure on haematological parameters and growth in clown knifefish (Chitala ornata, Gray 1831) |
| title_sort | effects of nitrite exposure on haematological parameters and growth in clown knifefish chitala ornata gray 1831 |
| topic | Clown knifefish growth haematological parameters nitrite |
| url | https://ctujs.ctu.edu.vn/index.php/ctujs/article/view/104 |
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