Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study
Emerging evidence has shown that microRNAs play pivotal roles in wound healing. MicroRNA-21 (miR-21) was previously found to upregulate in order to fulfill an anti-inflammation role for wounds. Exosomal miRNAs have been identified and explored as essential markers for diagnostic medicine. However, t...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/12/9822 |
| _version_ | 1797594363655618560 |
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| author | Shin-Chen Pan Chi-Hung Lai Van-Truc Vu Cao-An Vu Chun-Jen Huang Chao-Min Cheng Wen-Yih Chen |
| author_facet | Shin-Chen Pan Chi-Hung Lai Van-Truc Vu Cao-An Vu Chun-Jen Huang Chao-Min Cheng Wen-Yih Chen |
| author_sort | Shin-Chen Pan |
| collection | DOAJ |
| description | Emerging evidence has shown that microRNAs play pivotal roles in wound healing. MicroRNA-21 (miR-21) was previously found to upregulate in order to fulfill an anti-inflammation role for wounds. Exosomal miRNAs have been identified and explored as essential markers for diagnostic medicine. However, the role of exosomal miR-21 in wounds has yet to be well studied. In order to facilitate the early management of poorly healing wounds, we developed an easy-to-use, rapid, paper-based microfluidic-exosomal miR-21 extraction device to determine wound prognosis in a timely manner. We isolated and then quantitatively examined exosomal miR-21 in wound fluids from normal tissues and acute and chronic wounds. Eight improving wounds displayed lower levels of exosomal miR-21 expression after wound debridement. However, four instances of increased exosomal miR-21 expression levels were notably associated with patients with poor healing wounds despite aggressive wound debridement, indicating a predictive role of tissue exosomal miR-21 for wound outcome. Paper-based nucleic acid extraction device provides a rapid and user-friendly approach for evaluating exosomal miR-21 in wound fluids as a means of monitoring wounds. Our data suggest that tissue exosomal miR-21 is a reliable marker for determining current wound status. |
| first_indexed | 2024-03-11T02:22:38Z |
| format | Article |
| id | doaj.art-8a1059547c4e451286dbbaba88ab60aa |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T02:22:38Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8a1059547c4e451286dbbaba88ab60aa2023-11-18T10:44:40ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-012412982210.3390/ijms24129822Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial StudyShin-Chen Pan0Chi-Hung Lai1Van-Truc Vu2Cao-An Vu3Chun-Jen Huang4Chao-Min Cheng5Wen-Yih Chen6Department of Surgery, Section of Plastic and Reconstructive Surgery, National Cheng Kung University Hospital, College of Medicine, International Center for Wound Repair and Regeneration, National Cheng Kung University, Tainan 704, TaiwanDepartment of Chemical and Materials Engineering, National Central University, Taoyuan 32001, TaiwanDepartment of Chemical and Materials Engineering, National Central University, Taoyuan 32001, TaiwanDepartment of Chemical and Materials Engineering, National Central University, Taoyuan 32001, TaiwanDepartment of Chemical and Materials Engineering, National Central University, Taoyuan 32001, TaiwanInstitute of Biomedical Engineering, National Tsing Hua University, Hsinchu 300, TaiwanDepartment of Chemical and Materials Engineering, National Central University, Taoyuan 32001, TaiwanEmerging evidence has shown that microRNAs play pivotal roles in wound healing. MicroRNA-21 (miR-21) was previously found to upregulate in order to fulfill an anti-inflammation role for wounds. Exosomal miRNAs have been identified and explored as essential markers for diagnostic medicine. However, the role of exosomal miR-21 in wounds has yet to be well studied. In order to facilitate the early management of poorly healing wounds, we developed an easy-to-use, rapid, paper-based microfluidic-exosomal miR-21 extraction device to determine wound prognosis in a timely manner. We isolated and then quantitatively examined exosomal miR-21 in wound fluids from normal tissues and acute and chronic wounds. Eight improving wounds displayed lower levels of exosomal miR-21 expression after wound debridement. However, four instances of increased exosomal miR-21 expression levels were notably associated with patients with poor healing wounds despite aggressive wound debridement, indicating a predictive role of tissue exosomal miR-21 for wound outcome. Paper-based nucleic acid extraction device provides a rapid and user-friendly approach for evaluating exosomal miR-21 in wound fluids as a means of monitoring wounds. Our data suggest that tissue exosomal miR-21 is a reliable marker for determining current wound status.https://www.mdpi.com/1422-0067/24/12/9822P-ELISAexosomemicroRNA-21wound healing |
| spellingShingle | Shin-Chen Pan Chi-Hung Lai Van-Truc Vu Cao-An Vu Chun-Jen Huang Chao-Min Cheng Wen-Yih Chen Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study International Journal of Molecular Sciences P-ELISA exosome microRNA-21 wound healing |
| title | Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study |
| title_full | Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study |
| title_fullStr | Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study |
| title_full_unstemmed | Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study |
| title_short | Paper-Based Exosomal MicroRNA-21 Detection for Wound Monitoring: A Proof of Concept and Clinical Validation Trial Study |
| title_sort | paper based exosomal microrna 21 detection for wound monitoring a proof of concept and clinical validation trial study |
| topic | P-ELISA exosome microRNA-21 wound healing |
| url | https://www.mdpi.com/1422-0067/24/12/9822 |
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