Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin
Summary: Ebola virus (EBOV) and Bundibugyo virus (BDBV) belong to the family Filoviridae and cause a severe disease in humans. We previously isolated a large panel of monoclonal antibodies from B cells of human survivors from the 2007 Uganda BDBV outbreak, 16 survivors from the 2014 EBOV outbreak in...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-10-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124723012664 |
| _version_ | 1797656130604761088 |
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| author | Rodrigo I. Santos Philipp A. Ilinykh Colette A. Pietzsch Adam J. Ronk Kai Huang Natalia A. Kuzmina Fuchun Zhou James E. Crowe Alexander Bukreyev |
| author_facet | Rodrigo I. Santos Philipp A. Ilinykh Colette A. Pietzsch Adam J. Ronk Kai Huang Natalia A. Kuzmina Fuchun Zhou James E. Crowe Alexander Bukreyev |
| author_sort | Rodrigo I. Santos |
| collection | DOAJ |
| description | Summary: Ebola virus (EBOV) and Bundibugyo virus (BDBV) belong to the family Filoviridae and cause a severe disease in humans. We previously isolated a large panel of monoclonal antibodies from B cells of human survivors from the 2007 Uganda BDBV outbreak, 16 survivors from the 2014 EBOV outbreak in the Democratic Republic of the Congo, and one survivor from the West African 2013–2016 EBOV epidemic. Here, we demonstrate that EBOV and BDBV are capable of spreading to neighboring cells through intercellular connections in a process that depends upon actin and T cell immunoglobulin and mucin 1 protein. We quantify spread through intercellular connections by immunofluorescence microscopy and flow cytometry. One of the antibodies, BDBV223, specific to the membrane-proximal external region, induces virus accumulation at the plasma membrane. The inhibiting activity of BDBV223 depends on BST2/tetherin. |
| first_indexed | 2024-03-11T17:24:54Z |
| format | Article |
| id | doaj.art-8a1292785f724a3abef3fab20ae45612 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-03-11T17:24:54Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-8a1292785f724a3abef3fab20ae456122023-10-19T04:21:57ZengElsevierCell Reports2211-12472023-10-014210113254Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherinRodrigo I. Santos0Philipp A. Ilinykh1Colette A. Pietzsch2Adam J. Ronk3Kai Huang4Natalia A. Kuzmina5Fuchun Zhou6James E. Crowe7Alexander Bukreyev8Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USADepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USADepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USADepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USADepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USADepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USADepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USAVanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, USA; Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA; Corresponding authorDepartment of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USA; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA; Corresponding authorSummary: Ebola virus (EBOV) and Bundibugyo virus (BDBV) belong to the family Filoviridae and cause a severe disease in humans. We previously isolated a large panel of monoclonal antibodies from B cells of human survivors from the 2007 Uganda BDBV outbreak, 16 survivors from the 2014 EBOV outbreak in the Democratic Republic of the Congo, and one survivor from the West African 2013–2016 EBOV epidemic. Here, we demonstrate that EBOV and BDBV are capable of spreading to neighboring cells through intercellular connections in a process that depends upon actin and T cell immunoglobulin and mucin 1 protein. We quantify spread through intercellular connections by immunofluorescence microscopy and flow cytometry. One of the antibodies, BDBV223, specific to the membrane-proximal external region, induces virus accumulation at the plasma membrane. The inhibiting activity of BDBV223 depends on BST2/tetherin.http://www.sciencedirect.com/science/article/pii/S2211124723012664CP: MicrobiologyCP: Immunology |
| spellingShingle | Rodrigo I. Santos Philipp A. Ilinykh Colette A. Pietzsch Adam J. Ronk Kai Huang Natalia A. Kuzmina Fuchun Zhou James E. Crowe Alexander Bukreyev Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin Cell Reports CP: Microbiology CP: Immunology |
| title | Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin |
| title_full | Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin |
| title_fullStr | Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin |
| title_full_unstemmed | Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin |
| title_short | Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin |
| title_sort | blocking of ebolavirus spread through intercellular connections by an mper specific antibody depends on bst2 tetherin |
| topic | CP: Microbiology CP: Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124723012664 |
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