Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammation
Abstract Asparagus cochinchinensis has been used to treat various diseases including fever, cough, kidney disease, breast cancer, inflammatory disease and brain disease, while IL-4 cytokine has been considered as key regulator on the skin homeostasis and the predisposition toward allergic skin infla...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2016-12-01
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| Series: | Laboratory Animal Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.5625/lar.2016.32.1.34 |
| _version_ | 1811289628441837568 |
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| author | Ji-Eun Sung Hyun-Ah Lee Ji-Eun Kim Jun Go Eun-Ji Seo Woo-Bin Yun Dong-Seob Kim Hong-Joo Son Chung-Yeoul Lee Hee-Seob Lee Dae-Youn Hwang |
| author_facet | Ji-Eun Sung Hyun-Ah Lee Ji-Eun Kim Jun Go Eun-Ji Seo Woo-Bin Yun Dong-Seob Kim Hong-Joo Son Chung-Yeoul Lee Hee-Seob Lee Dae-Youn Hwang |
| author_sort | Ji-Eun Sung |
| collection | DOAJ |
| description | Abstract Asparagus cochinchinensis has been used to treat various diseases including fever, cough, kidney disease, breast cancer, inflammatory disease and brain disease, while IL-4 cytokine has been considered as key regulator on the skin homeostasis and the predisposition toward allergic skin inflammation. However, few studies have investigated its effects and IL-4 correlation on skin inflammation to date. To quantitatively evaluate the suppressive effects of ethyl acetate extracts of A. cochinchinensis (EaEAC) on phthalic anhydride (PA)-induced skin inflammation and investigate the role of IL-4 during their action mechanism, alterations in general phenotype biomarkers and luciferase-derived signals were measured in IL-4/Luc/CNS-1 transgenic (Tg) mice with PA-induced skin inflammation after treatment with EaEAC for 2 weeks. Key phenotype markers including lymph node weight, immunoglobulin E (IgE) concentration, epidermis thickness and number of infiltrated mast cells were significantly decreased in the PA+EaEAC treated group compared with the PA+Vehicle treated group. In addition, expression of IL-1ß and TNF-a was also decreased in the PA+EaEAC cotreated group, compared to PA+Vehicle treated group. Furthermore, a significant decrease in the luciferase signal derived from IL-4 promoter was detected in the abdominal region, submandibular lymph node and mesenteric lymph node of the PA+EaEAC treated group, compared to PA+Vehicle treated group. Taken together, these results suggest that EaEAC treatment could successfully improve PA-induced skin inflammation of IL-4/Luc/CNS-1 Tg mice, and that IL-4 cytokine plays a key role in the therapeutic process of EaEAC. |
| first_indexed | 2024-04-13T03:58:50Z |
| format | Article |
| id | doaj.art-8a12f6ab0d1a4547bcf680a14b08b9b1 |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-04-13T03:58:50Z |
| publishDate | 2016-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-8a12f6ab0d1a4547bcf680a14b08b9b12022-12-22T03:03:33ZengBMCLaboratory Animal Research2233-76602016-12-01321344510.5625/lar.2016.32.1.34Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammationJi-Eun Sung0Hyun-Ah Lee1Ji-Eun Kim2Jun Go3Eun-Ji Seo4Woo-Bin Yun5Dong-Seob Kim6Hong-Joo Son7Chung-Yeoul Lee8Hee-Seob Lee9Dae-Youn Hwang10Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National UniversityGangrim OrganicsCollege of Human Ecology, Pusan National UniversityDepartment of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National UniversityAbstract Asparagus cochinchinensis has been used to treat various diseases including fever, cough, kidney disease, breast cancer, inflammatory disease and brain disease, while IL-4 cytokine has been considered as key regulator on the skin homeostasis and the predisposition toward allergic skin inflammation. However, few studies have investigated its effects and IL-4 correlation on skin inflammation to date. To quantitatively evaluate the suppressive effects of ethyl acetate extracts of A. cochinchinensis (EaEAC) on phthalic anhydride (PA)-induced skin inflammation and investigate the role of IL-4 during their action mechanism, alterations in general phenotype biomarkers and luciferase-derived signals were measured in IL-4/Luc/CNS-1 transgenic (Tg) mice with PA-induced skin inflammation after treatment with EaEAC for 2 weeks. Key phenotype markers including lymph node weight, immunoglobulin E (IgE) concentration, epidermis thickness and number of infiltrated mast cells were significantly decreased in the PA+EaEAC treated group compared with the PA+Vehicle treated group. In addition, expression of IL-1ß and TNF-a was also decreased in the PA+EaEAC cotreated group, compared to PA+Vehicle treated group. Furthermore, a significant decrease in the luciferase signal derived from IL-4 promoter was detected in the abdominal region, submandibular lymph node and mesenteric lymph node of the PA+EaEAC treated group, compared to PA+Vehicle treated group. Taken together, these results suggest that EaEAC treatment could successfully improve PA-induced skin inflammation of IL-4/Luc/CNS-1 Tg mice, and that IL-4 cytokine plays a key role in the therapeutic process of EaEAC.http://link.springer.com/article/10.5625/lar.2016.32.1.34Asparagus cochinchinensisskin inflammationIL-4/Luc/CNS-1 transgenic micephthalic anhydrideIgE |
| spellingShingle | Ji-Eun Sung Hyun-Ah Lee Ji-Eun Kim Jun Go Eun-Ji Seo Woo-Bin Yun Dong-Seob Kim Hong-Joo Son Chung-Yeoul Lee Hee-Seob Lee Dae-Youn Hwang Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammation Laboratory Animal Research Asparagus cochinchinensis skin inflammation IL-4/Luc/CNS-1 transgenic mice phthalic anhydride IgE |
| title | Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammation |
| title_full | Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammation |
| title_fullStr | Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammation |
| title_full_unstemmed | Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammation |
| title_short | Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammation |
| title_sort | therapeutic effect of ethyl acetate extract from asparagus cochinchinensis on phthalic anhydride induced skin inflammation |
| topic | Asparagus cochinchinensis skin inflammation IL-4/Luc/CNS-1 transgenic mice phthalic anhydride IgE |
| url | http://link.springer.com/article/10.5625/lar.2016.32.1.34 |
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