Targeted Deletion of Centrin in Leishmania braziliensis Using CRISPR-Cas9-Based Editing

Targeted Deletion of Centrin in Leishmania braziliensis Using CRISPR-Cas9-Based Editing

Leishmania braziliensis is the main causative agent of Tegumentary Leishmaniasis in the Americas. However, difficulties related to genome manipulation, experimental infection, and parasite growth have so far limited studies with this species. CRISPR-Cas9-based technology has made genome editing more...

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Bibliographic Details
Main Authors: Rohit Sharma, Francys Avendaño Rangel, João Luís Reis-Cunha, Larissa Pinheiro Marques, Claudio P. Figueira, Pedro B. Borba, Sayonara M. Viana, Tom Beneke, Daniella C. Bartholomeu, Camila I. de Oliveira
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-02-01
Series:Frontiers in Cellular and Infection Microbiology
Subjects:
LeishGEedit
leishmaniasis
genetic manipulation
attenuation
vaccine development
Online Access:https://www.frontiersin.org/articles/10.3389/fcimb.2021.790418/full

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