Early diagnosis of leptospirosis
A 25-years old man from Zagreb, Croatia, was admitted to the University Hospital for Infectious Diseases four days after the onset of symptoms such as fever, intense pain in the calves and anuria. The patient owned a rabbit and, before the onset of the disease, repaired some rubber pipes damaged by...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2014-06-01
|
| Series: | Infectious Disease Reports |
| Subjects: | |
| Online Access: | http://www.pagepress.org/journals/index.php/idr/article/view/5156 |
| _version_ | 1818425075463880704 |
|---|---|
| author | Andrea Babic-Erceg Diana Karlovic-Martinkovic Marija Santini Zdenka Persic Tatjana Vilibic-Cavlek |
| author_facet | Andrea Babic-Erceg Diana Karlovic-Martinkovic Marija Santini Zdenka Persic Tatjana Vilibic-Cavlek |
| author_sort | Andrea Babic-Erceg |
| collection | DOAJ |
| description | A 25-years old man from Zagreb, Croatia, was admitted to the University Hospital for Infectious Diseases four days after the onset of symptoms such as fever, intense pain in the calves and anuria. The patient owned a rabbit and, before the onset of the disease, repaired some rubber pipes damaged by rodents. At admission, he had a severe clinical picture with fever, hypotension, jaundice, immobility, and pain in leg muscles. Treatment with ceftriaxone was initiated in combination with volume restitution. Renal failure soon ensued. Consequently continuous venovenous hemodiaphiltration therapy was performed. Due to acute respiratory distress syndrome, the patient was mechanically ventilated. The patient’s condition gradually improved and he recovered fully from multi-organ failure. Diagnosis was confirmed by a microscopic agglutination test (MAT) covering 15 leptospira serovars and real-time polymerase-chain reaction (PCR). The first serum sample taken on day 6 tested negative for leptospira, while PCR showed positive results for leptospiral DNA. The second serum sample taken on day 13 tested positive for serovar Canicola serogroup Canicola, serovar Patoc, serovar Grippotyphosa serogroup Grippotyphosa and serovar Tarassovi serogroup Tarassovi (titre 4000, 4000, 1000 and 2000, respectively), while PCR was negative. This report highlights the benefits of combining MAT and PCR methods in early diagnosis of leptospirosis. |
| first_indexed | 2024-12-14T14:08:10Z |
| format | Article |
| id | doaj.art-8a1a0ea696ab409eb9675331da812194 |
| institution | Directory Open Access Journal |
| issn | 2036-7430 2036-7449 |
| language | English |
| last_indexed | 2024-12-14T14:08:10Z |
| publishDate | 2014-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Infectious Disease Reports |
| spelling | doaj.art-8a1a0ea696ab409eb9675331da8121942022-12-21T22:58:25ZengMDPI AGInfectious Disease Reports2036-74302036-74492014-06-016210.4081/idr.2014.51562861Early diagnosis of leptospirosisAndrea Babic-Erceg0Diana Karlovic-Martinkovic1Marija Santini2Zdenka Persic3Tatjana Vilibic-Cavlek4Croatian National Institute of Public Health, ZagrebCroatian National Institute of Public Health, ZagrebUniversity Hospital for Infectious Diseases Dr. Fran Mihaljevic, ZagrebCroatian National Institute of Public Health, ZagrebCroatian National Institute of Public Health, ZagrebA 25-years old man from Zagreb, Croatia, was admitted to the University Hospital for Infectious Diseases four days after the onset of symptoms such as fever, intense pain in the calves and anuria. The patient owned a rabbit and, before the onset of the disease, repaired some rubber pipes damaged by rodents. At admission, he had a severe clinical picture with fever, hypotension, jaundice, immobility, and pain in leg muscles. Treatment with ceftriaxone was initiated in combination with volume restitution. Renal failure soon ensued. Consequently continuous venovenous hemodiaphiltration therapy was performed. Due to acute respiratory distress syndrome, the patient was mechanically ventilated. The patient’s condition gradually improved and he recovered fully from multi-organ failure. Diagnosis was confirmed by a microscopic agglutination test (MAT) covering 15 leptospira serovars and real-time polymerase-chain reaction (PCR). The first serum sample taken on day 6 tested negative for leptospira, while PCR showed positive results for leptospiral DNA. The second serum sample taken on day 13 tested positive for serovar Canicola serogroup Canicola, serovar Patoc, serovar Grippotyphosa serogroup Grippotyphosa and serovar Tarassovi serogroup Tarassovi (titre 4000, 4000, 1000 and 2000, respectively), while PCR was negative. This report highlights the benefits of combining MAT and PCR methods in early diagnosis of leptospirosis.http://www.pagepress.org/journals/index.php/idr/article/view/5156leptospirosis, microscopic agglutination test, polymerase chain reaction |
| spellingShingle | Andrea Babic-Erceg Diana Karlovic-Martinkovic Marija Santini Zdenka Persic Tatjana Vilibic-Cavlek Early diagnosis of leptospirosis Infectious Disease Reports leptospirosis, microscopic agglutination test, polymerase chain reaction |
| title | Early diagnosis of leptospirosis |
| title_full | Early diagnosis of leptospirosis |
| title_fullStr | Early diagnosis of leptospirosis |
| title_full_unstemmed | Early diagnosis of leptospirosis |
| title_short | Early diagnosis of leptospirosis |
| title_sort | early diagnosis of leptospirosis |
| topic | leptospirosis, microscopic agglutination test, polymerase chain reaction |
| url | http://www.pagepress.org/journals/index.php/idr/article/view/5156 |
| work_keys_str_mv | AT andreababicerceg earlydiagnosisofleptospirosis AT dianakarlovicmartinkovic earlydiagnosisofleptospirosis AT marijasantini earlydiagnosisofleptospirosis AT zdenkapersic earlydiagnosisofleptospirosis AT tatjanavilibiccavlek earlydiagnosisofleptospirosis |