Chromosomal Polymorphism and Speciation: The Case of the Genus <i>Mazama</i> (Cetartiodactyla; Cervidae)
Chromosomal polymorphism plays a major role in speciation processes in mammals with high rates of karyotypic evolution, as observed in the family Cervidae. One remarkable example is the genus <i>Mazama</i> that comprises wide inter- and intra-specific chromosomal variability. To evaluate...
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MDPI AG
2021-01-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/12/2/165 |
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| author | David Javier Galindo Gabriela Siqueira Martins Miluse Vozdova Halina Cernohorska Svatava Kubickova Agda Maria Bernegossi Dita Kadlcikova Jiri Rubes José Maurício Barbanti Duarte |
| author_facet | David Javier Galindo Gabriela Siqueira Martins Miluse Vozdova Halina Cernohorska Svatava Kubickova Agda Maria Bernegossi Dita Kadlcikova Jiri Rubes José Maurício Barbanti Duarte |
| author_sort | David Javier Galindo |
| collection | DOAJ |
| description | Chromosomal polymorphism plays a major role in speciation processes in mammals with high rates of karyotypic evolution, as observed in the family Cervidae. One remarkable example is the genus <i>Mazama</i> that comprises wide inter- and intra-specific chromosomal variability. To evaluate the impact of chromosomal polymorphisms as reproductive barriers within the genus <i>Mazama</i>, inter-specific hybrids between <i>Mazama gouazoubira</i> and <i>Mazama nemorivaga</i> (MGO × MNE) and intra-specific hybrids between cytotypes of <i>Mazama americana</i> (MAM) differing by a tandem (TF) or centric fusion (Robertsonian translocations—RT) were evaluated. MGO × MNE hybrid fertility was evaluated by the seminal quality and testicular histology. MAM hybrids estimation of the meiotic segregation products was performed by sperm-FISH analysis. MGO × MNE hybrids analyses showed different degrees of fertility reduction, from severe subfertility to complete sterility. Regarding MAM, RT, and TF carriers showed a mean value for alternate segregation rate of 97.74%, and 67.23%, and adjacent segregation rate of 1.80%, and 29.07%, respectively. Our results suggested an efficient post-zygotic barrier represented by severe fertility reduction for MGO × MNE and MAM with heterozygous TF. Nevertheless, RT did not show a severe effect on the reproductive fitness in MAM. Our data support the validity of MGO and MNE as different species and reveals cryptic species within MAM. |
| first_indexed | 2024-03-09T03:39:35Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T03:39:35Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-8a1bd155372a429fae30d1893eb6df932023-12-03T14:42:41ZengMDPI AGGenes2073-44252021-01-0112216510.3390/genes12020165Chromosomal Polymorphism and Speciation: The Case of the Genus <i>Mazama</i> (Cetartiodactyla; Cervidae)David Javier Galindo0Gabriela Siqueira Martins1Miluse Vozdova2Halina Cernohorska3Svatava Kubickova4Agda Maria Bernegossi5Dita Kadlcikova6Jiri Rubes7José Maurício Barbanti Duarte8Núcleo de Pesquisa e Conservação de Cervídeos, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista—NUPECCE/FCAV/UNESP, 14884-900 Jaboticabal, BrazilLaboratório de Dosagens Hormonais, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo—FMVZ/USP, 05508-270 São Paulo, BrazilDepartment of Genetics and Reproductive Biotechnologies, Central European Institute of Technology—Veterinary Research Institute, 621-00 Brno, Czech RepublicDepartment of Genetics and Reproductive Biotechnologies, Central European Institute of Technology—Veterinary Research Institute, 621-00 Brno, Czech RepublicDepartment of Genetics and Reproductive Biotechnologies, Central European Institute of Technology—Veterinary Research Institute, 621-00 Brno, Czech RepublicNúcleo de Pesquisa e Conservação de Cervídeos, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista—NUPECCE/FCAV/UNESP, 14884-900 Jaboticabal, BrazilDepartment of Genetics and Reproductive Biotechnologies, Central European Institute of Technology—Veterinary Research Institute, 621-00 Brno, Czech RepublicDepartment of Genetics and Reproductive Biotechnologies, Central European Institute of Technology—Veterinary Research Institute, 621-00 Brno, Czech RepublicNúcleo de Pesquisa e Conservação de Cervídeos, Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista—NUPECCE/FCAV/UNESP, 14884-900 Jaboticabal, BrazilChromosomal polymorphism plays a major role in speciation processes in mammals with high rates of karyotypic evolution, as observed in the family Cervidae. One remarkable example is the genus <i>Mazama</i> that comprises wide inter- and intra-specific chromosomal variability. To evaluate the impact of chromosomal polymorphisms as reproductive barriers within the genus <i>Mazama</i>, inter-specific hybrids between <i>Mazama gouazoubira</i> and <i>Mazama nemorivaga</i> (MGO × MNE) and intra-specific hybrids between cytotypes of <i>Mazama americana</i> (MAM) differing by a tandem (TF) or centric fusion (Robertsonian translocations—RT) were evaluated. MGO × MNE hybrid fertility was evaluated by the seminal quality and testicular histology. MAM hybrids estimation of the meiotic segregation products was performed by sperm-FISH analysis. MGO × MNE hybrids analyses showed different degrees of fertility reduction, from severe subfertility to complete sterility. Regarding MAM, RT, and TF carriers showed a mean value for alternate segregation rate of 97.74%, and 67.23%, and adjacent segregation rate of 1.80%, and 29.07%, respectively. Our results suggested an efficient post-zygotic barrier represented by severe fertility reduction for MGO × MNE and MAM with heterozygous TF. Nevertheless, RT did not show a severe effect on the reproductive fitness in MAM. Our data support the validity of MGO and MNE as different species and reveals cryptic species within MAM.https://www.mdpi.com/2073-4425/12/2/165cytogeneticshybridspost-zygotic barriersperm-FISHNeotropical deer |
| spellingShingle | David Javier Galindo Gabriela Siqueira Martins Miluse Vozdova Halina Cernohorska Svatava Kubickova Agda Maria Bernegossi Dita Kadlcikova Jiri Rubes José Maurício Barbanti Duarte Chromosomal Polymorphism and Speciation: The Case of the Genus <i>Mazama</i> (Cetartiodactyla; Cervidae) Genes cytogenetics hybrids post-zygotic barrier sperm-FISH Neotropical deer |
| title | Chromosomal Polymorphism and Speciation: The Case of the Genus <i>Mazama</i> (Cetartiodactyla; Cervidae) |
| title_full | Chromosomal Polymorphism and Speciation: The Case of the Genus <i>Mazama</i> (Cetartiodactyla; Cervidae) |
| title_fullStr | Chromosomal Polymorphism and Speciation: The Case of the Genus <i>Mazama</i> (Cetartiodactyla; Cervidae) |
| title_full_unstemmed | Chromosomal Polymorphism and Speciation: The Case of the Genus <i>Mazama</i> (Cetartiodactyla; Cervidae) |
| title_short | Chromosomal Polymorphism and Speciation: The Case of the Genus <i>Mazama</i> (Cetartiodactyla; Cervidae) |
| title_sort | chromosomal polymorphism and speciation the case of the genus i mazama i cetartiodactyla cervidae |
| topic | cytogenetics hybrids post-zygotic barrier sperm-FISH Neotropical deer |
| url | https://www.mdpi.com/2073-4425/12/2/165 |
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