Application of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review
Abstract Background The baculovirus expression vector system has been developed for expressing a wide range of proteins, including enzymes, glycoproteins, recombinant viruses, and vaccines. The availability of the SARS-CoV-2 genome sequence has enabled the synthesis of SARS-CoV2 proteins in a baculo...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | https://doi.org/10.1186/s43141-022-00368-7 |
| _version_ | 1797203307715887104 |
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| author | Muhammad Azharuddin Azali Salmah Mohamed Azian Harun Faezahtul Arbaeyah Hussain Shaharum Shamsuddin Muhammad Farid Johan |
| author_facet | Muhammad Azharuddin Azali Salmah Mohamed Azian Harun Faezahtul Arbaeyah Hussain Shaharum Shamsuddin Muhammad Farid Johan |
| author_sort | Muhammad Azharuddin Azali |
| collection | DOAJ |
| description | Abstract Background The baculovirus expression vector system has been developed for expressing a wide range of proteins, including enzymes, glycoproteins, recombinant viruses, and vaccines. The availability of the SARS-CoV-2 genome sequence has enabled the synthesis of SARS-CoV2 proteins in a baculovirus-insect cell platform for various applications. Main body of the abstract The most cloned SARS-CoV-2 protein is the spike protein, which plays a critical role in SARS-CoV-2 infection. It is available in its whole length or as subunits like S1 or the receptor-binding domain (RBD). Non-structural proteins (Nsps), another recombinant SARS-CoV-2 protein generated by the baculovirus expression vector system (BEV), are used in the identification of new medications or the repurposing of existing therapies for the treatment of COVID-19. Non-SARS-CoV-2 proteins generated by BEV for SARS-CoV-2 diagnosis or treatment include moloney murine leukemia virus reverse transcriptase (MMLVRT), angiotensin converting enzyme 2 (ACE2), therapeutic proteins, and recombinant antibodies. The recombinant proteins were modified to boost the yield or to stabilize the protein. Conclusion This review covers the wide application of the recombinant protein produced using the baculovirus expression technology for COVID-19 research. A lot of improvements have been made to produce functional proteins with high yields. However, there is still room for improvement and there are parts of this field of research that have not been investigated yet. |
| first_indexed | 2024-04-24T08:17:15Z |
| format | Article |
| id | doaj.art-8a1ce42435ec4868a8bf00a4277f46d0 |
| institution | Directory Open Access Journal |
| issn | 2090-5920 |
| language | English |
| last_indexed | 2024-04-24T08:17:15Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj.art-8a1ce42435ec4868a8bf00a4277f46d02024-04-17T03:22:48ZengElsevierJournal of Genetic Engineering and Biotechnology2090-59202022-07-0120112010.1186/s43141-022-00368-7Application of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a reviewMuhammad Azharuddin Azali0Salmah Mohamed1Azian Harun2Faezahtul Arbaeyah Hussain3Shaharum Shamsuddin4Muhammad Farid Johan5Department of Haematology, School of Medical Sciences, Universiti Sains MalaysiaSchool of Agriculture Science and Biotechnology, Faculty of Bioresources and Food Industry, Universiti Sultan Zainal AbidinDepartment of Medical Microbiology and Parasitology, School of Medical Sciences, Universiti Sains MalaysiaDepartment of Pathology, School of Medical Sciences, Universiti Sains MalaysiaSchool of Health Sciences, Universiti Sains MalaysiaDepartment of Haematology, School of Medical Sciences, Universiti Sains MalaysiaAbstract Background The baculovirus expression vector system has been developed for expressing a wide range of proteins, including enzymes, glycoproteins, recombinant viruses, and vaccines. The availability of the SARS-CoV-2 genome sequence has enabled the synthesis of SARS-CoV2 proteins in a baculovirus-insect cell platform for various applications. Main body of the abstract The most cloned SARS-CoV-2 protein is the spike protein, which plays a critical role in SARS-CoV-2 infection. It is available in its whole length or as subunits like S1 or the receptor-binding domain (RBD). Non-structural proteins (Nsps), another recombinant SARS-CoV-2 protein generated by the baculovirus expression vector system (BEV), are used in the identification of new medications or the repurposing of existing therapies for the treatment of COVID-19. Non-SARS-CoV-2 proteins generated by BEV for SARS-CoV-2 diagnosis or treatment include moloney murine leukemia virus reverse transcriptase (MMLVRT), angiotensin converting enzyme 2 (ACE2), therapeutic proteins, and recombinant antibodies. The recombinant proteins were modified to boost the yield or to stabilize the protein. Conclusion This review covers the wide application of the recombinant protein produced using the baculovirus expression technology for COVID-19 research. A lot of improvements have been made to produce functional proteins with high yields. However, there is still room for improvement and there are parts of this field of research that have not been investigated yet.https://doi.org/10.1186/s43141-022-00368-7SARS-CoV-2COVID-19Baculovirus expression systemSpike proteinNon-structural proteinAngiotensin converting enzyme 2 |
| spellingShingle | Muhammad Azharuddin Azali Salmah Mohamed Azian Harun Faezahtul Arbaeyah Hussain Shaharum Shamsuddin Muhammad Farid Johan Application of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review Journal of Genetic Engineering and Biotechnology SARS-CoV-2 COVID-19 Baculovirus expression system Spike protein Non-structural protein Angiotensin converting enzyme 2 |
| title | Application of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review |
| title_full | Application of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review |
| title_fullStr | Application of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review |
| title_full_unstemmed | Application of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review |
| title_short | Application of Baculovirus Expression Vector system (BEV) for COVID-19 diagnostics and therapeutics: a review |
| title_sort | application of baculovirus expression vector system bev for covid 19 diagnostics and therapeutics a review |
| topic | SARS-CoV-2 COVID-19 Baculovirus expression system Spike protein Non-structural protein Angiotensin converting enzyme 2 |
| url | https://doi.org/10.1186/s43141-022-00368-7 |
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