Differential expression profiles of miRNA in the serum of sarcopenic rats
As the geriatric population and life expectancy increase, the interest in preventing geriatric diseases, such as sarcopenia, is increasing. However, the causes of sarcopenia are unclear, and current diagnostic methods for sarcopenia are unreliable. We hypothesized that the changes in the expression...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-07-01
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| Series: | Biochemistry and Biophysics Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580822000516 |
| _version_ | 1818209391471493120 |
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| author | Wonjong Yu Min-kyu Yang Dong Jun Sung Tae Jun Park Myungchul Kim Eustache Ntigura Sung Hea Kim Bokyung Kim Sang Woong Park Young Min Bae |
| author_facet | Wonjong Yu Min-kyu Yang Dong Jun Sung Tae Jun Park Myungchul Kim Eustache Ntigura Sung Hea Kim Bokyung Kim Sang Woong Park Young Min Bae |
| author_sort | Wonjong Yu |
| collection | DOAJ |
| description | As the geriatric population and life expectancy increase, the interest in preventing geriatric diseases, such as sarcopenia, is increasing. However, the causes of sarcopenia are unclear, and current diagnostic methods for sarcopenia are unreliable. We hypothesized that the changes in the expression of certain miRNAs may be associated with the pathophysiology of sarcopenia. Herein, we analyzed the miRNA expression profiles in the blood of young (3-months-old) healthy rats, old sarcopenic (17-months-old) rats, and age-matched (17-months-old) control rats. The changes in miRNA expression levels were analyzed using Bowtie 2 software. A total of 523 miRNAs were detected in the rat serum. Using scatter plots and clustering heatmap data, we found 130 miRNAs that were differentially expressed in sarcopenic rats (>2-fold change) compared to the expression in young healthy and age-matched control rats. With a threshold of >5-fold change, we identified 14 upregulated miRNAs, including rno-miR-133b-3p, rno-miR-133a-3p, rno-miR-133c, rno-miR-208a-3p, and rno-miR434-5p among others in the serum of sarcopenic rats. A protein network map based on these 14 miRNAs identified the genes involved in skeletal muscle differentiation, among which Notch1, Egr2, and Myocd represented major nodes. The data obtained in this study are potentially useful for the early diagnosis of sarcopenia and for the identification of novel therapeutic targets for the treatment and/or prevention of sarcopenia. |
| first_indexed | 2024-12-12T04:59:58Z |
| format | Article |
| id | doaj.art-8a1e89bb9f1d415694c9d7eeb8a899ee |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-12T04:59:58Z |
| publishDate | 2022-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-8a1e89bb9f1d415694c9d7eeb8a899ee2022-12-22T00:37:15ZengElsevierBiochemistry and Biophysics Reports2405-58082022-07-0130101251Differential expression profiles of miRNA in the serum of sarcopenic ratsWonjong Yu0Min-kyu Yang1Dong Jun Sung2Tae Jun Park3Myungchul Kim4Eustache Ntigura5Sung Hea Kim6Bokyung Kim7Sang Woong Park8Young Min Bae9Department of Physical Therapy, Eulji University, South KoreaDepartment of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, Chungju, South KoreaDepartment of Sport and Health Studies, College of Biomedical and Health Science, Konkuk University, Chungju, Republic of KoreaDepartment of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, Chungju, South KoreaDepartment of Physical Therapy, Eulji University, South KoreaDepartment of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, Chungju, South Korea; Department of Preventive and Community Dentistry, School of Dentistry, College of Medicine and Health Sciences, University of Rwanda, Kigali, RwandaDepartment of Cardiology, Konkuk University School of Medicine, Seoul, South KoreaDepartment of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, Chungju, South KoreaDepartment of Emergency Medical Services, Eulji University, South Korea; Corresponding author.Department of Physiology, KU Open Innovation Center, Research Institute of Medical Science, Konkuk University School of Medicine, Chungju, South Korea; Corresponding author.As the geriatric population and life expectancy increase, the interest in preventing geriatric diseases, such as sarcopenia, is increasing. However, the causes of sarcopenia are unclear, and current diagnostic methods for sarcopenia are unreliable. We hypothesized that the changes in the expression of certain miRNAs may be associated with the pathophysiology of sarcopenia. Herein, we analyzed the miRNA expression profiles in the blood of young (3-months-old) healthy rats, old sarcopenic (17-months-old) rats, and age-matched (17-months-old) control rats. The changes in miRNA expression levels were analyzed using Bowtie 2 software. A total of 523 miRNAs were detected in the rat serum. Using scatter plots and clustering heatmap data, we found 130 miRNAs that were differentially expressed in sarcopenic rats (>2-fold change) compared to the expression in young healthy and age-matched control rats. With a threshold of >5-fold change, we identified 14 upregulated miRNAs, including rno-miR-133b-3p, rno-miR-133a-3p, rno-miR-133c, rno-miR-208a-3p, and rno-miR434-5p among others in the serum of sarcopenic rats. A protein network map based on these 14 miRNAs identified the genes involved in skeletal muscle differentiation, among which Notch1, Egr2, and Myocd represented major nodes. The data obtained in this study are potentially useful for the early diagnosis of sarcopenia and for the identification of novel therapeutic targets for the treatment and/or prevention of sarcopenia.http://www.sciencedirect.com/science/article/pii/S2405580822000516SarcopeniaAgingDiagnosticsmicroRNANext-generation sequencingSkeletal muscle |
| spellingShingle | Wonjong Yu Min-kyu Yang Dong Jun Sung Tae Jun Park Myungchul Kim Eustache Ntigura Sung Hea Kim Bokyung Kim Sang Woong Park Young Min Bae Differential expression profiles of miRNA in the serum of sarcopenic rats Biochemistry and Biophysics Reports Sarcopenia Aging Diagnostics microRNA Next-generation sequencing Skeletal muscle |
| title | Differential expression profiles of miRNA in the serum of sarcopenic rats |
| title_full | Differential expression profiles of miRNA in the serum of sarcopenic rats |
| title_fullStr | Differential expression profiles of miRNA in the serum of sarcopenic rats |
| title_full_unstemmed | Differential expression profiles of miRNA in the serum of sarcopenic rats |
| title_short | Differential expression profiles of miRNA in the serum of sarcopenic rats |
| title_sort | differential expression profiles of mirna in the serum of sarcopenic rats |
| topic | Sarcopenia Aging Diagnostics microRNA Next-generation sequencing Skeletal muscle |
| url | http://www.sciencedirect.com/science/article/pii/S2405580822000516 |
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