Bioprinting and Preliminary Testing of Highly Reproducible Novel Bioink for Potential Skin Regeneration
Three-dimensional (3D) bioprinting is considered as a novel approach in biofabricating cell-laden constructs that could potentially be used to promote skin regeneration following injury. In this study, a novel crosslinked chitosan (CH)–genipin (GE) bioink laden with keratinocyte and human dermal fib...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/12/6/550 |
| _version_ | 1797565437751328768 |
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| author | Forough Hafezi Susan Shorter Atabak Ghanizadeh Tabriz Andrew Hurt Victoria Elmes Joshua Boateng Dennis Douroumis |
| author_facet | Forough Hafezi Susan Shorter Atabak Ghanizadeh Tabriz Andrew Hurt Victoria Elmes Joshua Boateng Dennis Douroumis |
| author_sort | Forough Hafezi |
| collection | DOAJ |
| description | Three-dimensional (3D) bioprinting is considered as a novel approach in biofabricating cell-laden constructs that could potentially be used to promote skin regeneration following injury. In this study, a novel crosslinked chitosan (CH)–genipin (GE) bioink laden with keratinocyte and human dermal fibroblast cells was developed and printed successfully using an extruder-based bioprinter. By altering the composition and degree of CH–GE crosslinking, bioink printability was further assessed and compared with a commercial bioink. Rheological analysis showed that the viscosity of the optimised bioink was in a suitable range that facilitated reproducible and reliable printing by applying low pressures ranging from 20–40 kPa. The application of low printing pressures proved vital for viability of cells loaded within the bioinks. Further characterisation using MTT assay showed that cells were still viable within the printed construct at 93% despite the crosslinking, processing and after subjecting to physiological conditions for seven days. The morphological study of the printed cells showed that they were mobile within the bioink. Furthermore, the multi-layered 3D printed constructs demonstrated excellent self-supportive structures in a consistent manner. |
| first_indexed | 2024-03-10T19:12:13Z |
| format | Article |
| id | doaj.art-8a20fa1902014e89b8bac381464dbf29 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T19:12:13Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-8a20fa1902014e89b8bac381464dbf292023-11-20T03:43:05ZengMDPI AGPharmaceutics1999-49232020-06-0112655010.3390/pharmaceutics12060550Bioprinting and Preliminary Testing of Highly Reproducible Novel Bioink for Potential Skin RegenerationForough Hafezi0Susan Shorter1Atabak Ghanizadeh Tabriz2Andrew Hurt3Victoria Elmes4Joshua Boateng5Dennis Douroumis6School of Science, Faculty of Engineering and Science, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UKSchool of Science, Faculty of Engineering and Science, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UKSchool of Science, Faculty of Engineering and Science, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UKSchool of Science, Faculty of Engineering and Science, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UKSchool of Science, Faculty of Engineering and Science, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UKSchool of Science, Faculty of Engineering and Science, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UKSchool of Science, Faculty of Engineering and Science, University of Greenwich, Chatham Maritime, Kent ME4 4TB, UKThree-dimensional (3D) bioprinting is considered as a novel approach in biofabricating cell-laden constructs that could potentially be used to promote skin regeneration following injury. In this study, a novel crosslinked chitosan (CH)–genipin (GE) bioink laden with keratinocyte and human dermal fibroblast cells was developed and printed successfully using an extruder-based bioprinter. By altering the composition and degree of CH–GE crosslinking, bioink printability was further assessed and compared with a commercial bioink. Rheological analysis showed that the viscosity of the optimised bioink was in a suitable range that facilitated reproducible and reliable printing by applying low pressures ranging from 20–40 kPa. The application of low printing pressures proved vital for viability of cells loaded within the bioinks. Further characterisation using MTT assay showed that cells were still viable within the printed construct at 93% despite the crosslinking, processing and after subjecting to physiological conditions for seven days. The morphological study of the printed cells showed that they were mobile within the bioink. Furthermore, the multi-layered 3D printed constructs demonstrated excellent self-supportive structures in a consistent manner.https://www.mdpi.com/1999-4923/12/6/5503D bioprintingbioinkchitosangenipinhuman dermal fibroblastsprimary epidermal keratinocytes |
| spellingShingle | Forough Hafezi Susan Shorter Atabak Ghanizadeh Tabriz Andrew Hurt Victoria Elmes Joshua Boateng Dennis Douroumis Bioprinting and Preliminary Testing of Highly Reproducible Novel Bioink for Potential Skin Regeneration Pharmaceutics 3D bioprinting bioink chitosan genipin human dermal fibroblasts primary epidermal keratinocytes |
| title | Bioprinting and Preliminary Testing of Highly Reproducible Novel Bioink for Potential Skin Regeneration |
| title_full | Bioprinting and Preliminary Testing of Highly Reproducible Novel Bioink for Potential Skin Regeneration |
| title_fullStr | Bioprinting and Preliminary Testing of Highly Reproducible Novel Bioink for Potential Skin Regeneration |
| title_full_unstemmed | Bioprinting and Preliminary Testing of Highly Reproducible Novel Bioink for Potential Skin Regeneration |
| title_short | Bioprinting and Preliminary Testing of Highly Reproducible Novel Bioink for Potential Skin Regeneration |
| title_sort | bioprinting and preliminary testing of highly reproducible novel bioink for potential skin regeneration |
| topic | 3D bioprinting bioink chitosan genipin human dermal fibroblasts primary epidermal keratinocytes |
| url | https://www.mdpi.com/1999-4923/12/6/550 |
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