Study on <i>ZmRPN10</i> Regulating Leaf Angle in Maize by RNA-Seq
Ubiquitin/proteasome-mediated proteolysis (UPP) plays a crucial role in almost all aspects of plant growth and development, proteasome subunit RPN10 mediates ubiquitination substrate recognition in the UPP process. The recognition pathway of ubiquitinated UPP substrate is different in different spec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/1/189 |
| _version_ | 1797431493711101952 |
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| author | Xiangzhuo Ji Bingbing Jin Zelong Zhuang Fangguo Chang Fang Wang Yunling Peng |
| author_facet | Xiangzhuo Ji Bingbing Jin Zelong Zhuang Fangguo Chang Fang Wang Yunling Peng |
| author_sort | Xiangzhuo Ji |
| collection | DOAJ |
| description | Ubiquitin/proteasome-mediated proteolysis (UPP) plays a crucial role in almost all aspects of plant growth and development, proteasome subunit RPN10 mediates ubiquitination substrate recognition in the UPP process. The recognition pathway of ubiquitinated UPP substrate is different in different species, which indicates that the mechanism and function of <i>RPN10</i> are different in different species. However, the homologous <i>ZmRPN10</i> in maize has not been studied. In this study, the changing of leaf angle and gene expression in leaves in maize wild-type B73 and mutant <i>rpn10</i> under exogenous brassinosteroids (BRs) were investigated. The regulation effect of BR on the leaf angle of <i>rpn10</i> was significantly stronger than that of B73. Transcriptome analysis showed that among the differentially expressed genes, CRE1, A-ARR and SnRK2 were significantly up-regulated, and PP2C, BRI1 AUX/IAA, JAZ and MYC2 were significantly down-regulated. This study revealed the regulation mechanism of <i>ZmRPN10</i> on maize leaf angle and provided a promising gene resource for maize breeding. |
| first_indexed | 2024-03-09T09:44:51Z |
| format | Article |
| id | doaj.art-8a2282f418f44522808cbe1ee1cb3652 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T09:44:51Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-8a2282f418f44522808cbe1ee1cb36522023-12-02T00:38:13ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124118910.3390/ijms24010189Study on <i>ZmRPN10</i> Regulating Leaf Angle in Maize by RNA-SeqXiangzhuo Ji0Bingbing Jin1Zelong Zhuang2Fangguo Chang3Fang Wang4Yunling Peng5College of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Agronomy, Gansu Agricultural University, Lanzhou 730070, ChinaUbiquitin/proteasome-mediated proteolysis (UPP) plays a crucial role in almost all aspects of plant growth and development, proteasome subunit RPN10 mediates ubiquitination substrate recognition in the UPP process. The recognition pathway of ubiquitinated UPP substrate is different in different species, which indicates that the mechanism and function of <i>RPN10</i> are different in different species. However, the homologous <i>ZmRPN10</i> in maize has not been studied. In this study, the changing of leaf angle and gene expression in leaves in maize wild-type B73 and mutant <i>rpn10</i> under exogenous brassinosteroids (BRs) were investigated. The regulation effect of BR on the leaf angle of <i>rpn10</i> was significantly stronger than that of B73. Transcriptome analysis showed that among the differentially expressed genes, CRE1, A-ARR and SnRK2 were significantly up-regulated, and PP2C, BRI1 AUX/IAA, JAZ and MYC2 were significantly down-regulated. This study revealed the regulation mechanism of <i>ZmRPN10</i> on maize leaf angle and provided a promising gene resource for maize breeding.https://www.mdpi.com/1422-0067/24/1/189maize (<i>Zea mays</i> L.)<i>ZmRPN10</i>leaf angleRNA-Seq |
| spellingShingle | Xiangzhuo Ji Bingbing Jin Zelong Zhuang Fangguo Chang Fang Wang Yunling Peng Study on <i>ZmRPN10</i> Regulating Leaf Angle in Maize by RNA-Seq International Journal of Molecular Sciences maize (<i>Zea mays</i> L.) <i>ZmRPN10</i> leaf angle RNA-Seq |
| title | Study on <i>ZmRPN10</i> Regulating Leaf Angle in Maize by RNA-Seq |
| title_full | Study on <i>ZmRPN10</i> Regulating Leaf Angle in Maize by RNA-Seq |
| title_fullStr | Study on <i>ZmRPN10</i> Regulating Leaf Angle in Maize by RNA-Seq |
| title_full_unstemmed | Study on <i>ZmRPN10</i> Regulating Leaf Angle in Maize by RNA-Seq |
| title_short | Study on <i>ZmRPN10</i> Regulating Leaf Angle in Maize by RNA-Seq |
| title_sort | study on i zmrpn10 i regulating leaf angle in maize by rna seq |
| topic | maize (<i>Zea mays</i> L.) <i>ZmRPN10</i> leaf angle RNA-Seq |
| url | https://www.mdpi.com/1422-0067/24/1/189 |
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