Complex of Lauric Acid Monoglyceride and Cinnamaldehyde Ameliorated Subclinical Necrotic Enteritis in Yellow-Feathered Broilers by Regulating Gut Morphology, Barrier, Inflammation and Serum Biochemistry
This experiment investigated the benefits of plant essential oil (EO) composed with lauric acid monoglyceride and cinnamaldehyde on necrotic enteritis-challenged broilers. A total of 180 1-day-old healthy yellow-feathered broilers were randomly divided into 4 groups with 3 replicates of 15 chicks ea...
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MDPI AG
2023-02-01
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| Online Access: | https://www.mdpi.com/2076-2615/13/3/516 |
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| author | Chaojun Zheng Gengsheng Xiao Xia Yan Ting Qiu Shun Liu Jiancun Ou Mingzhu Cen Li Gong Jiansheng Shi Huihua Zhang |
| author_facet | Chaojun Zheng Gengsheng Xiao Xia Yan Ting Qiu Shun Liu Jiancun Ou Mingzhu Cen Li Gong Jiansheng Shi Huihua Zhang |
| author_sort | Chaojun Zheng |
| collection | DOAJ |
| description | This experiment investigated the benefits of plant essential oil (EO) composed with lauric acid monoglyceride and cinnamaldehyde on necrotic enteritis-challenged broilers. A total of 180 1-day-old healthy yellow-feathered broilers were randomly divided into 4 groups with 3 replicates of 15 chicks each. The experimental groups were as follows: the control group (CON) was fed with the basal diet and was not challenged by <i>Eimeria acervulina</i> (EA) and <i>Clostridium perfringens</i> (CP); CPEA group was also fed with a basal diet, but infected with CP and EA; CPEA_EO350 group and CPEA_EO500 group were fed with a basal diet supplemented with 350 and 500 mg/kg EO, respectively, and all infected with CP and EA. On the 7th day, each bird in the CPEA group, CPEA_EO350 group and CPEA_EO500 group was orally administrated with 1 mL <i>Eimeria acervulina</i> containing 5000 oocytes/mL, and the birds of the CON group were orally administrated with 1 mL normal saline. From the 15th day, 1 mL of CP type A CVCC-2030 strain (about 5 × 10<sup>8</sup> cfu/mL) was orally inoculated into each bird of the CPEA, CPEA_EO350 and CPEA_EO500 groups for three consecutive days. Similarly, the CON group was orally given 1 mL of normal saline. The CPEA stimulation reduced the average daily gain (ADG) of broilers, increased the feed-to-gain ratio (F:G), and increased the intestinal lesions of the broilers (<i>p</i> < 0.01), indicating that CPEA stimulation was clinically successful. Compared with the CPEA group, the ADG of CPEA_EO350 and CPEA_EO500 increased, the F:G decreased (<i>p</i> < 0.01), and the intestinal score of CPEA_EO500 decreased (<i>p</i> < 0.01). The expression of the tight junction protein of the jejunum and ileum on 21d was upregulated (<i>p</i> < 0.01), and the expression of jejunum inflammation factors TNF-α on 21d and jejunum and ileum inflammatory factor IL-6 on 28d were also downregulated. The CPEA_EO350 and CPEA_EO500 increased antioxidant capacity. To sum up, 350 and 500 mg/kg of lauric acid monoglyceride and cinnamaldehyde complex plant essential oils can improve ADG and F:G, improve intestinal morphology and the body’s antioxidant capacity, and downregulate the expression of inflammatory factors. The concentration of 500 mg/kg performed even better. |
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| spelling | doaj.art-8a273d82191a45e0861755e4c13b85732023-11-16T16:01:56ZengMDPI AGAnimals2076-26152023-02-0113351610.3390/ani13030516Complex of Lauric Acid Monoglyceride and Cinnamaldehyde Ameliorated Subclinical Necrotic Enteritis in Yellow-Feathered Broilers by Regulating Gut Morphology, Barrier, Inflammation and Serum BiochemistryChaojun Zheng0Gengsheng Xiao1Xia Yan2Ting Qiu3Shun Liu4Jiancun Ou5Mingzhu Cen6Li Gong7Jiansheng Shi8Huihua Zhang9School of Life Science and Engineering, Foshan University, Foshan 528000, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528000, ChinaLaboratory of Livestock and Poultry Breeding, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510000, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528000, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528000, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528000, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528000, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528000, ChinaCollege of Life Science and Food Engineering, Hebei University of Engineering, Handan 056038, ChinaSchool of Life Science and Engineering, Foshan University, Foshan 528000, ChinaThis experiment investigated the benefits of plant essential oil (EO) composed with lauric acid monoglyceride and cinnamaldehyde on necrotic enteritis-challenged broilers. A total of 180 1-day-old healthy yellow-feathered broilers were randomly divided into 4 groups with 3 replicates of 15 chicks each. The experimental groups were as follows: the control group (CON) was fed with the basal diet and was not challenged by <i>Eimeria acervulina</i> (EA) and <i>Clostridium perfringens</i> (CP); CPEA group was also fed with a basal diet, but infected with CP and EA; CPEA_EO350 group and CPEA_EO500 group were fed with a basal diet supplemented with 350 and 500 mg/kg EO, respectively, and all infected with CP and EA. On the 7th day, each bird in the CPEA group, CPEA_EO350 group and CPEA_EO500 group was orally administrated with 1 mL <i>Eimeria acervulina</i> containing 5000 oocytes/mL, and the birds of the CON group were orally administrated with 1 mL normal saline. From the 15th day, 1 mL of CP type A CVCC-2030 strain (about 5 × 10<sup>8</sup> cfu/mL) was orally inoculated into each bird of the CPEA, CPEA_EO350 and CPEA_EO500 groups for three consecutive days. Similarly, the CON group was orally given 1 mL of normal saline. The CPEA stimulation reduced the average daily gain (ADG) of broilers, increased the feed-to-gain ratio (F:G), and increased the intestinal lesions of the broilers (<i>p</i> < 0.01), indicating that CPEA stimulation was clinically successful. Compared with the CPEA group, the ADG of CPEA_EO350 and CPEA_EO500 increased, the F:G decreased (<i>p</i> < 0.01), and the intestinal score of CPEA_EO500 decreased (<i>p</i> < 0.01). The expression of the tight junction protein of the jejunum and ileum on 21d was upregulated (<i>p</i> < 0.01), and the expression of jejunum inflammation factors TNF-α on 21d and jejunum and ileum inflammatory factor IL-6 on 28d were also downregulated. The CPEA_EO350 and CPEA_EO500 increased antioxidant capacity. To sum up, 350 and 500 mg/kg of lauric acid monoglyceride and cinnamaldehyde complex plant essential oils can improve ADG and F:G, improve intestinal morphology and the body’s antioxidant capacity, and downregulate the expression of inflammatory factors. The concentration of 500 mg/kg performed even better.https://www.mdpi.com/2076-2615/13/3/516cinnamaldehydelauric acid monoglyceridenecrotic enteritisgut morphologygut barrierplant essential oil |
| spellingShingle | Chaojun Zheng Gengsheng Xiao Xia Yan Ting Qiu Shun Liu Jiancun Ou Mingzhu Cen Li Gong Jiansheng Shi Huihua Zhang Complex of Lauric Acid Monoglyceride and Cinnamaldehyde Ameliorated Subclinical Necrotic Enteritis in Yellow-Feathered Broilers by Regulating Gut Morphology, Barrier, Inflammation and Serum Biochemistry Animals cinnamaldehyde lauric acid monoglyceride necrotic enteritis gut morphology gut barrier plant essential oil |
| title | Complex of Lauric Acid Monoglyceride and Cinnamaldehyde Ameliorated Subclinical Necrotic Enteritis in Yellow-Feathered Broilers by Regulating Gut Morphology, Barrier, Inflammation and Serum Biochemistry |
| title_full | Complex of Lauric Acid Monoglyceride and Cinnamaldehyde Ameliorated Subclinical Necrotic Enteritis in Yellow-Feathered Broilers by Regulating Gut Morphology, Barrier, Inflammation and Serum Biochemistry |
| title_fullStr | Complex of Lauric Acid Monoglyceride and Cinnamaldehyde Ameliorated Subclinical Necrotic Enteritis in Yellow-Feathered Broilers by Regulating Gut Morphology, Barrier, Inflammation and Serum Biochemistry |
| title_full_unstemmed | Complex of Lauric Acid Monoglyceride and Cinnamaldehyde Ameliorated Subclinical Necrotic Enteritis in Yellow-Feathered Broilers by Regulating Gut Morphology, Barrier, Inflammation and Serum Biochemistry |
| title_short | Complex of Lauric Acid Monoglyceride and Cinnamaldehyde Ameliorated Subclinical Necrotic Enteritis in Yellow-Feathered Broilers by Regulating Gut Morphology, Barrier, Inflammation and Serum Biochemistry |
| title_sort | complex of lauric acid monoglyceride and cinnamaldehyde ameliorated subclinical necrotic enteritis in yellow feathered broilers by regulating gut morphology barrier inflammation and serum biochemistry |
| topic | cinnamaldehyde lauric acid monoglyceride necrotic enteritis gut morphology gut barrier plant essential oil |
| url | https://www.mdpi.com/2076-2615/13/3/516 |
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