Arterial relaxation is coupled to inhibition of mitochondrial fission in arterial smooth muscle cells: comparison of vasorelaxant effects of verapamil and phentolamine
Mitochondria are morphologically dynamic organelles which undergo fission and fusion processes. Our previous study found that arterial constriction was always accompanied by increased mitochondrial fission in smooth muscle cells, whereas inhibition of mitochondrial fission in smooth muscle cells was...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2017-05-01
|
| Series: | Acta Pharmaceutica Sinica B |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211383516303550 |
| _version_ | 1818138798183153664 |
|---|---|
| author | Jing Jin Xin Shen Yu Tai Shanliang Li Mingyu Liu Changlin Zhen Xiuchen Xuan Xiyue Zhang Nan Hu Xinzi Zhang Deli Dong |
| author_facet | Jing Jin Xin Shen Yu Tai Shanliang Li Mingyu Liu Changlin Zhen Xiuchen Xuan Xiyue Zhang Nan Hu Xinzi Zhang Deli Dong |
| author_sort | Jing Jin |
| collection | DOAJ |
| description | Mitochondria are morphologically dynamic organelles which undergo fission and fusion processes. Our previous study found that arterial constriction was always accompanied by increased mitochondrial fission in smooth muscle cells, whereas inhibition of mitochondrial fission in smooth muscle cells was associated with arterial relaxation. Here, we used the typical vasorelaxants, verapamil and phentolamine, to further confirm the coupling between arterial constriction and mitochondrial fission in rat aorta. Results showed that phentolamine but not verapamil induced vasorelaxation in phenylephrine (PE)-induced rat thoracic aorta constriction. Verapamil, but not phentolamine, induced vasorelaxation in high K+ (KPSS)-induced rat thoracic aorta constriction. Pre-treatment with phentolamine prevented PE- but not KPSS-induced aorta constriction and pre-treatment with verapamil prevented both PE- and KPSS-induced aorta constriction. Transmission electron microscopy (TEM) results showed that verapamil but not phentolamine inhibited KPSS-induced excessive mitochondrial fission in aortic smooth muscle cells, and verapamil prevented both PE- and KPSS-induced excessive mitochondrial fission in aortic smooth muscle cells. Verapamil inhibited KPSS-induced excessive mitochondrial fission in cultured vascular smooth muscle cells (A10). These results further demonstrate that arterial relaxation is coupled to inhibition of mitochondrial fission in arterial smooth muscle cells. |
| first_indexed | 2024-12-11T10:17:55Z |
| format | Article |
| id | doaj.art-8a29a2ef03484bb4970e5c2c85a65e92 |
| institution | Directory Open Access Journal |
| issn | 2211-3835 2211-3843 |
| language | English |
| last_indexed | 2024-12-11T10:17:55Z |
| publishDate | 2017-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Acta Pharmaceutica Sinica B |
| spelling | doaj.art-8a29a2ef03484bb4970e5c2c85a65e922022-12-22T01:11:34ZengElsevierActa Pharmaceutica Sinica B2211-38352211-38432017-05-017331932510.1016/j.apsb.2016.12.009Arterial relaxation is coupled to inhibition of mitochondrial fission in arterial smooth muscle cells: comparison of vasorelaxant effects of verapamil and phentolamineJing Jin0Xin Shen1Yu Tai2Shanliang Li3Mingyu Liu4Changlin Zhen5Xiuchen Xuan6Xiyue Zhang7Nan Hu8Xinzi Zhang9Deli Dong10Department of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150086, ChinaDepartment of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150086, ChinaDepartment of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150086, ChinaDepartment of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150086, ChinaDepartment of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150086, ChinaDepartment of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150086, ChinaDepartment of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150086, ChinaDepartment of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150086, ChinaDepartment of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150086, ChinaDepartment of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150086, ChinaDepartment of Pharmacology (the State-Province Key Laboratories of Biomedicine-Pharmaceutics of China, Key Laboratory of Cardiovascular Research, Ministry of Education), College of Pharmacy, Harbin Medical University, Harbin 150086, ChinaMitochondria are morphologically dynamic organelles which undergo fission and fusion processes. Our previous study found that arterial constriction was always accompanied by increased mitochondrial fission in smooth muscle cells, whereas inhibition of mitochondrial fission in smooth muscle cells was associated with arterial relaxation. Here, we used the typical vasorelaxants, verapamil and phentolamine, to further confirm the coupling between arterial constriction and mitochondrial fission in rat aorta. Results showed that phentolamine but not verapamil induced vasorelaxation in phenylephrine (PE)-induced rat thoracic aorta constriction. Verapamil, but not phentolamine, induced vasorelaxation in high K+ (KPSS)-induced rat thoracic aorta constriction. Pre-treatment with phentolamine prevented PE- but not KPSS-induced aorta constriction and pre-treatment with verapamil prevented both PE- and KPSS-induced aorta constriction. Transmission electron microscopy (TEM) results showed that verapamil but not phentolamine inhibited KPSS-induced excessive mitochondrial fission in aortic smooth muscle cells, and verapamil prevented both PE- and KPSS-induced excessive mitochondrial fission in aortic smooth muscle cells. Verapamil inhibited KPSS-induced excessive mitochondrial fission in cultured vascular smooth muscle cells (A10). These results further demonstrate that arterial relaxation is coupled to inhibition of mitochondrial fission in arterial smooth muscle cells.http://www.sciencedirect.com/science/article/pii/S2211383516303550ArteryMitochondrial fissionPhentolamineVasorelaxationVerapamil |
| spellingShingle | Jing Jin Xin Shen Yu Tai Shanliang Li Mingyu Liu Changlin Zhen Xiuchen Xuan Xiyue Zhang Nan Hu Xinzi Zhang Deli Dong Arterial relaxation is coupled to inhibition of mitochondrial fission in arterial smooth muscle cells: comparison of vasorelaxant effects of verapamil and phentolamine Acta Pharmaceutica Sinica B Artery Mitochondrial fission Phentolamine Vasorelaxation Verapamil |
| title | Arterial relaxation is coupled to inhibition of mitochondrial fission in arterial smooth muscle cells: comparison of vasorelaxant effects of verapamil and phentolamine |
| title_full | Arterial relaxation is coupled to inhibition of mitochondrial fission in arterial smooth muscle cells: comparison of vasorelaxant effects of verapamil and phentolamine |
| title_fullStr | Arterial relaxation is coupled to inhibition of mitochondrial fission in arterial smooth muscle cells: comparison of vasorelaxant effects of verapamil and phentolamine |
| title_full_unstemmed | Arterial relaxation is coupled to inhibition of mitochondrial fission in arterial smooth muscle cells: comparison of vasorelaxant effects of verapamil and phentolamine |
| title_short | Arterial relaxation is coupled to inhibition of mitochondrial fission in arterial smooth muscle cells: comparison of vasorelaxant effects of verapamil and phentolamine |
| title_sort | arterial relaxation is coupled to inhibition of mitochondrial fission in arterial smooth muscle cells comparison of vasorelaxant effects of verapamil and phentolamine |
| topic | Artery Mitochondrial fission Phentolamine Vasorelaxation Verapamil |
| url | http://www.sciencedirect.com/science/article/pii/S2211383516303550 |
| work_keys_str_mv | AT jingjin arterialrelaxationiscoupledtoinhibitionofmitochondrialfissioninarterialsmoothmusclecellscomparisonofvasorelaxanteffectsofverapamilandphentolamine AT xinshen arterialrelaxationiscoupledtoinhibitionofmitochondrialfissioninarterialsmoothmusclecellscomparisonofvasorelaxanteffectsofverapamilandphentolamine AT yutai arterialrelaxationiscoupledtoinhibitionofmitochondrialfissioninarterialsmoothmusclecellscomparisonofvasorelaxanteffectsofverapamilandphentolamine AT shanliangli arterialrelaxationiscoupledtoinhibitionofmitochondrialfissioninarterialsmoothmusclecellscomparisonofvasorelaxanteffectsofverapamilandphentolamine AT mingyuliu arterialrelaxationiscoupledtoinhibitionofmitochondrialfissioninarterialsmoothmusclecellscomparisonofvasorelaxanteffectsofverapamilandphentolamine AT changlinzhen arterialrelaxationiscoupledtoinhibitionofmitochondrialfissioninarterialsmoothmusclecellscomparisonofvasorelaxanteffectsofverapamilandphentolamine AT xiuchenxuan arterialrelaxationiscoupledtoinhibitionofmitochondrialfissioninarterialsmoothmusclecellscomparisonofvasorelaxanteffectsofverapamilandphentolamine AT xiyuezhang arterialrelaxationiscoupledtoinhibitionofmitochondrialfissioninarterialsmoothmusclecellscomparisonofvasorelaxanteffectsofverapamilandphentolamine AT nanhu arterialrelaxationiscoupledtoinhibitionofmitochondrialfissioninarterialsmoothmusclecellscomparisonofvasorelaxanteffectsofverapamilandphentolamine AT xinzizhang arterialrelaxationiscoupledtoinhibitionofmitochondrialfissioninarterialsmoothmusclecellscomparisonofvasorelaxanteffectsofverapamilandphentolamine AT delidong arterialrelaxationiscoupledtoinhibitionofmitochondrialfissioninarterialsmoothmusclecellscomparisonofvasorelaxanteffectsofverapamilandphentolamine |