Characterization of Bacterial Cellulose Produced by <i>Komagataeibacter maltaceti</i> P285 Isolated from Contaminated Honey Wine
Bacterial cellulose (BC), a biopolymer, is synthesized by BC-producing bacteria. Almost all producing strains are classified in the family <i>Acetobacteraceae</i>. In this study, bacterial strain P285 was isolated from contaminated honey wine in a honey factory in northern Thailand. Base...
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MDPI AG
2022-02-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/3/528 |
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| author | Narumol Thongwai Wirapong Futui Nanthiwa Ladpala Benjamat Sirichai Anuwat Weechan Jirapat Kanklai Patthanasak Rungsirivanich |
| author_facet | Narumol Thongwai Wirapong Futui Nanthiwa Ladpala Benjamat Sirichai Anuwat Weechan Jirapat Kanklai Patthanasak Rungsirivanich |
| author_sort | Narumol Thongwai |
| collection | DOAJ |
| description | Bacterial cellulose (BC), a biopolymer, is synthesized by BC-producing bacteria. Almost all producing strains are classified in the family <i>Acetobacteraceae</i>. In this study, bacterial strain P285 was isolated from contaminated honey wine in a honey factory in northern Thailand. Based on 16S rRNA gene sequence identification, the strain P285 revealed 99.8% identity with <i>Komagataeibacter maltaceti</i> LMG 1529 <sup>T</sup>. <i>K. maltaceti</i> P285 produced the maximum BC production at 20–30 °C and an initial media pH of 9.0. The highest BC production in modified mineral salt medium (MSM) was exhibited when glucose (16%, <i>w</i>/<i>v</i>) and yeast extract (3.2%, <i>w</i>/<i>v</i>) were applied as carbon and nitrogen sources, respectively. When sugarcane (8–16%, <i>w</i>/<i>v</i>) or honey (ratio of honey to water = 1: 4) supplemented with yeast extract was used, the BC production was greater. The characterization of BC synthesized by <i>K. maltaceti</i> P285 was undertaken using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectrometry. Meanwhile, X-ray diffraction results confirmed the presence of crystalline cellulose (2θ = 18.330, 21.390 and 22.640°). The maximum temperature of BC degradation was observed at 314 °C. Tensile properties analysis of hydrated and dried BC showed breaking strength of 1.49 and 0.66 MPa, respectively. These results demonstrated that <i>K. maltaceti</i> P285 has a high potential for BC production especially when grown in high initial media pH. Therefore, the strain would be suitable as an agent to make BC, the value-added product in the related factories. |
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| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T13:16:35Z |
| publishDate | 2022-02-01 |
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| spelling | doaj.art-8a2a04aa54ee443682308d6314e5d8282023-11-30T21:35:33ZengMDPI AGMicroorganisms2076-26072022-02-0110352810.3390/microorganisms10030528Characterization of Bacterial Cellulose Produced by <i>Komagataeibacter maltaceti</i> P285 Isolated from Contaminated Honey WineNarumol Thongwai0Wirapong Futui1Nanthiwa Ladpala2Benjamat Sirichai3Anuwat Weechan4Jirapat Kanklai5Patthanasak Rungsirivanich6Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandDepartment of Biology, Faculty of Science, Chiang Mai University, Chiang Mai 50200, ThailandBacterial cellulose (BC), a biopolymer, is synthesized by BC-producing bacteria. Almost all producing strains are classified in the family <i>Acetobacteraceae</i>. In this study, bacterial strain P285 was isolated from contaminated honey wine in a honey factory in northern Thailand. Based on 16S rRNA gene sequence identification, the strain P285 revealed 99.8% identity with <i>Komagataeibacter maltaceti</i> LMG 1529 <sup>T</sup>. <i>K. maltaceti</i> P285 produced the maximum BC production at 20–30 °C and an initial media pH of 9.0. The highest BC production in modified mineral salt medium (MSM) was exhibited when glucose (16%, <i>w</i>/<i>v</i>) and yeast extract (3.2%, <i>w</i>/<i>v</i>) were applied as carbon and nitrogen sources, respectively. When sugarcane (8–16%, <i>w</i>/<i>v</i>) or honey (ratio of honey to water = 1: 4) supplemented with yeast extract was used, the BC production was greater. The characterization of BC synthesized by <i>K. maltaceti</i> P285 was undertaken using scanning electron microscopy (SEM) and Fourier transform infrared (FTIR) spectrometry. Meanwhile, X-ray diffraction results confirmed the presence of crystalline cellulose (2θ = 18.330, 21.390 and 22.640°). The maximum temperature of BC degradation was observed at 314 °C. Tensile properties analysis of hydrated and dried BC showed breaking strength of 1.49 and 0.66 MPa, respectively. These results demonstrated that <i>K. maltaceti</i> P285 has a high potential for BC production especially when grown in high initial media pH. Therefore, the strain would be suitable as an agent to make BC, the value-added product in the related factories.https://www.mdpi.com/2076-2607/10/3/528<i>Acetobacter xylinum</i>biopolymerhoneymeadoptimizationsugarcane |
| spellingShingle | Narumol Thongwai Wirapong Futui Nanthiwa Ladpala Benjamat Sirichai Anuwat Weechan Jirapat Kanklai Patthanasak Rungsirivanich Characterization of Bacterial Cellulose Produced by <i>Komagataeibacter maltaceti</i> P285 Isolated from Contaminated Honey Wine Microorganisms <i>Acetobacter xylinum</i> biopolymer honey mead optimization sugarcane |
| title | Characterization of Bacterial Cellulose Produced by <i>Komagataeibacter maltaceti</i> P285 Isolated from Contaminated Honey Wine |
| title_full | Characterization of Bacterial Cellulose Produced by <i>Komagataeibacter maltaceti</i> P285 Isolated from Contaminated Honey Wine |
| title_fullStr | Characterization of Bacterial Cellulose Produced by <i>Komagataeibacter maltaceti</i> P285 Isolated from Contaminated Honey Wine |
| title_full_unstemmed | Characterization of Bacterial Cellulose Produced by <i>Komagataeibacter maltaceti</i> P285 Isolated from Contaminated Honey Wine |
| title_short | Characterization of Bacterial Cellulose Produced by <i>Komagataeibacter maltaceti</i> P285 Isolated from Contaminated Honey Wine |
| title_sort | characterization of bacterial cellulose produced by i komagataeibacter maltaceti i p285 isolated from contaminated honey wine |
| topic | <i>Acetobacter xylinum</i> biopolymer honey mead optimization sugarcane |
| url | https://www.mdpi.com/2076-2607/10/3/528 |
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