Genome-Wide Identification and Evolution Analysis of the <i>CYP76</i> Subfamily in Rice (<i>Oryza sativa</i>)
The <i>CYP76</i> subfamily, a member of the <i>CYP</i> superfamily, plays crucial roles in the biosynthesis of phytohormones in plants, involving biosynthesis of secondary metabolites, hormone signaling, and response to environmental stresses. Here, we conducted a genome-wide...
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MDPI AG
2023-05-01
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| author | Mingao Zhou Yifei Jiang Xuhui Liu Weilong Kong Chenhao Zhang Jian Yang Simin Ke Yangsheng Li |
| author_facet | Mingao Zhou Yifei Jiang Xuhui Liu Weilong Kong Chenhao Zhang Jian Yang Simin Ke Yangsheng Li |
| author_sort | Mingao Zhou |
| collection | DOAJ |
| description | The <i>CYP76</i> subfamily, a member of the <i>CYP</i> superfamily, plays crucial roles in the biosynthesis of phytohormones in plants, involving biosynthesis of secondary metabolites, hormone signaling, and response to environmental stresses. Here, we conducted a genome-wide analysis of the <i>CYP76</i> subfamily in seven AA genome species: <i>Oryza sativa</i> ssp. <i>japonica</i>, <i>Oryza sativa</i> ssp. <i>indica, Oryza rufipogon</i>, <i>Oryza glaberrima</i>, <i>Oryza meridionalis</i>, <i>Oryza barthii</i>, and <i>Oryza glumaepatula.</i> These were identified and classified into three groups, and it was found that Group 1 contained the largest number of members. Analysis of cis-acting elements revealed a large number of elements related to jasmonic acid and light response. The gene duplication analysis revealed that the <i>CYP76</i> subfamily expanded mainly in SD/WGD and tandem forms and underwent strong purifying selection during evolution. Expression pattern analysis of <i>OsCYP76s</i> in various developmental stages revealed that the majority of <i>OsCYP76s</i> exhibit relatively restricted expression patterns in leaves and roots. We further analyzed the expression of CYP76s in <i>O. sativa</i>, <i>japonica</i>, and <i>O. sativa</i>, <i>indica</i> under cold, flooding, drought, and salt abiotic stresses by qRT-PCR. We found that <i>OsCYP76-11</i> showed a huge increase in relative expression after drought and salt stresses. After flooding stress, <i>OsiCYP76-4</i> showed a greater increase in expression compared to other genes. CYP76 in <i>japonica</i> and <i>indica</i> showed different response patterns to the same abiotic stresses, revealing functional divergence in the gene family during evolution; these may be the key genes responsible for the differences in tolerance to indica japonica. Our results provide valuable insights into the functional diversity and evolutionary history of the <i>CYP76</i> subfamily and pave the way for the development of new strategies for improving stress tolerance and agronomic traits in rice. |
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| language | English |
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| spelling | doaj.art-8a2a6ec3794646ada86f341fa63c43fa2023-11-18T01:37:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-05-012410852210.3390/ijms24108522Genome-Wide Identification and Evolution Analysis of the <i>CYP76</i> Subfamily in Rice (<i>Oryza sativa</i>)Mingao Zhou0Yifei Jiang1Xuhui Liu2Weilong Kong3Chenhao Zhang4Jian Yang5Simin Ke6Yangsheng Li7State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaState Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, ChinaThe <i>CYP76</i> subfamily, a member of the <i>CYP</i> superfamily, plays crucial roles in the biosynthesis of phytohormones in plants, involving biosynthesis of secondary metabolites, hormone signaling, and response to environmental stresses. Here, we conducted a genome-wide analysis of the <i>CYP76</i> subfamily in seven AA genome species: <i>Oryza sativa</i> ssp. <i>japonica</i>, <i>Oryza sativa</i> ssp. <i>indica, Oryza rufipogon</i>, <i>Oryza glaberrima</i>, <i>Oryza meridionalis</i>, <i>Oryza barthii</i>, and <i>Oryza glumaepatula.</i> These were identified and classified into three groups, and it was found that Group 1 contained the largest number of members. Analysis of cis-acting elements revealed a large number of elements related to jasmonic acid and light response. The gene duplication analysis revealed that the <i>CYP76</i> subfamily expanded mainly in SD/WGD and tandem forms and underwent strong purifying selection during evolution. Expression pattern analysis of <i>OsCYP76s</i> in various developmental stages revealed that the majority of <i>OsCYP76s</i> exhibit relatively restricted expression patterns in leaves and roots. We further analyzed the expression of CYP76s in <i>O. sativa</i>, <i>japonica</i>, and <i>O. sativa</i>, <i>indica</i> under cold, flooding, drought, and salt abiotic stresses by qRT-PCR. We found that <i>OsCYP76-11</i> showed a huge increase in relative expression after drought and salt stresses. After flooding stress, <i>OsiCYP76-4</i> showed a greater increase in expression compared to other genes. CYP76 in <i>japonica</i> and <i>indica</i> showed different response patterns to the same abiotic stresses, revealing functional divergence in the gene family during evolution; these may be the key genes responsible for the differences in tolerance to indica japonica. Our results provide valuable insights into the functional diversity and evolutionary history of the <i>CYP76</i> subfamily and pave the way for the development of new strategies for improving stress tolerance and agronomic traits in rice.https://www.mdpi.com/1422-0067/24/10/8522abiotic stresscytochrome P450 proteinexpression patternsgene family<i>Oryza genus</i> |
| spellingShingle | Mingao Zhou Yifei Jiang Xuhui Liu Weilong Kong Chenhao Zhang Jian Yang Simin Ke Yangsheng Li Genome-Wide Identification and Evolution Analysis of the <i>CYP76</i> Subfamily in Rice (<i>Oryza sativa</i>) International Journal of Molecular Sciences abiotic stress cytochrome P450 protein expression patterns gene family <i>Oryza genus</i> |
| title | Genome-Wide Identification and Evolution Analysis of the <i>CYP76</i> Subfamily in Rice (<i>Oryza sativa</i>) |
| title_full | Genome-Wide Identification and Evolution Analysis of the <i>CYP76</i> Subfamily in Rice (<i>Oryza sativa</i>) |
| title_fullStr | Genome-Wide Identification and Evolution Analysis of the <i>CYP76</i> Subfamily in Rice (<i>Oryza sativa</i>) |
| title_full_unstemmed | Genome-Wide Identification and Evolution Analysis of the <i>CYP76</i> Subfamily in Rice (<i>Oryza sativa</i>) |
| title_short | Genome-Wide Identification and Evolution Analysis of the <i>CYP76</i> Subfamily in Rice (<i>Oryza sativa</i>) |
| title_sort | genome wide identification and evolution analysis of the i cyp76 i subfamily in rice i oryza sativa i |
| topic | abiotic stress cytochrome P450 protein expression patterns gene family <i>Oryza genus</i> |
| url | https://www.mdpi.com/1422-0067/24/10/8522 |
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