Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol Anggrek
Soft rot disease in orchid plants is a factor in decreasing orchid production. One way of biological control that has the potential to spur growth is to utilize endophytic bacteria. This study aims to describe the diversity of orchid endophytic bacteria that have the potential as growth promoters a...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2023-08-01
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| Series: | Jurnal Ilmu Pertanian Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/JIPI/article/view/47202 |
| _version_ | 1797672761311625216 |
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| author | Mery Irianti Amba Linggi Tri Joko Jaka Widada |
| author_facet | Mery Irianti Amba Linggi Tri Joko Jaka Widada |
| author_sort | Mery Irianti Amba Linggi |
| collection | DOAJ |
| description |
Soft rot disease in orchid plants is a factor in decreasing orchid production. One way of biological control that has the potential to spur growth is to utilize endophytic bacteria. This study aims to describe the diversity of orchid endophytic bacteria that have the potential as growth promoters and biocontrol agents. Observation parameters include indole acetic acid (IAA) production, inorganic phosphate dissolution, ammonia production of orchid endophytic bacteria, and antagonism of endophytic bacteria to pathogenic bacteria that cause soft rot in vitro. The diversity of orchid endophytic bacteria was analyzed using rep-PCR with BOX-A1R primer. The results showed that 10 isolates of orchid endophytic bacteria were able to produce IAA with a concentration of 79–321 μg/mL, able to dissolve phosphate, and produce ammonia. In antagonism testing against soft rot-causing bacteria, all isolates were able to form an inhibitory zone with a diameter of 3–7.2 cm. Furthermore, diversity analysis using rep-PCR showed that isolates of endophytic bacteria DnPh5, BgCt2, BgVt10, DnLp7, DnBl1, and DnAr4 had a uniform DNA band pattern so that they were grouped together. Meanwhile, isolates of TbPh7, IbtPhm1, DnDr2, and AkOc1 showed different DNA band patterns, indicating that the four isolates were different species or subspecies of bacteria. In dendrogram analysis, DnPh5, BgCt2, BgVt10, DnLp7, DnBl1, and DnAr4 isolates have a similarity of 100%. The similarity of isolates IbtPhm1, DnDr2, and AkOc1 was 90%, 79%, and 68%, respectively, against the other six isolates. TbPh7 isolate is an isolate with a low level of similarity to other isolates, which is about 51%.
Keywords: orchid, endophytic bacteria, growth promoter, biocontrol agent, rep-PCR
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| first_indexed | 2024-03-11T21:34:55Z |
| format | Article |
| id | doaj.art-8a2b4787e438434b844f4413ccbb14e7 |
| institution | Directory Open Access Journal |
| issn | 0853-4217 2443-3462 |
| language | English |
| last_indexed | 2024-03-11T21:34:55Z |
| publishDate | 2023-08-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Jurnal Ilmu Pertanian Indonesia |
| spelling | doaj.art-8a2b4787e438434b844f4413ccbb14e72023-09-27T03:22:46ZengBogor Agricultural UniversityJurnal Ilmu Pertanian Indonesia0853-42172443-34622023-08-0128410.18343/jipi.28.4.675Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol AnggrekMery Irianti Amba Linggi0Tri Joko1Jaka Widada2Program Studi Agroteknologi, STIPER Santo Thomas Aquinas, Jl. Akuatan-Kemiri I No.4, Papua 99352Departemen Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Universitas Gadjah Mada, Jalan Bulaksumur, Yogyakarta 55281Departemen Mikrobiologi, Fakultas Pertanian, Universitas Gadjah Mada, Jl. Bulaksumur, Yogyakarta 55281 Soft rot disease in orchid plants is a factor in decreasing orchid production. One way of biological control that has the potential to spur growth is to utilize endophytic bacteria. This study aims to describe the diversity of orchid endophytic bacteria that have the potential as growth promoters and biocontrol agents. Observation parameters include indole acetic acid (IAA) production, inorganic phosphate dissolution, ammonia production of orchid endophytic bacteria, and antagonism of endophytic bacteria to pathogenic bacteria that cause soft rot in vitro. The diversity of orchid endophytic bacteria was analyzed using rep-PCR with BOX-A1R primer. The results showed that 10 isolates of orchid endophytic bacteria were able to produce IAA with a concentration of 79–321 μg/mL, able to dissolve phosphate, and produce ammonia. In antagonism testing against soft rot-causing bacteria, all isolates were able to form an inhibitory zone with a diameter of 3–7.2 cm. Furthermore, diversity analysis using rep-PCR showed that isolates of endophytic bacteria DnPh5, BgCt2, BgVt10, DnLp7, DnBl1, and DnAr4 had a uniform DNA band pattern so that they were grouped together. Meanwhile, isolates of TbPh7, IbtPhm1, DnDr2, and AkOc1 showed different DNA band patterns, indicating that the four isolates were different species or subspecies of bacteria. In dendrogram analysis, DnPh5, BgCt2, BgVt10, DnLp7, DnBl1, and DnAr4 isolates have a similarity of 100%. The similarity of isolates IbtPhm1, DnDr2, and AkOc1 was 90%, 79%, and 68%, respectively, against the other six isolates. TbPh7 isolate is an isolate with a low level of similarity to other isolates, which is about 51%. Keywords: orchid, endophytic bacteria, growth promoter, biocontrol agent, rep-PCR https://journal.ipb.ac.id/index.php/JIPI/article/view/47202 |
| spellingShingle | Mery Irianti Amba Linggi Tri Joko Jaka Widada Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol Anggrek Jurnal Ilmu Pertanian Indonesia |
| title | Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol Anggrek |
| title_full | Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol Anggrek |
| title_fullStr | Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol Anggrek |
| title_full_unstemmed | Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol Anggrek |
| title_short | Potensi dan Keragaman Bakteri Endofit sebagai Agens Pemacu Pertumbuhan dan Biokontrol Anggrek |
| title_sort | potensi dan keragaman bakteri endofit sebagai agens pemacu pertumbuhan dan biokontrol anggrek |
| url | https://journal.ipb.ac.id/index.php/JIPI/article/view/47202 |
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