Isolation of Potential Probiotic <i>Bacillus</i> spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile Tilapia
This study aimed to investigate the potential probiotic <i>Bacillus</i> spp. from the intestine of Nile tilapia in order to construct a recombinant probiotic for the enhancement of the Nile tilapia immune response. One hundred bacterial isolates from the intestine of Nile tilapia were ch...
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MDPI AG
2023-03-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/6/986 |
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| author | Chatsirin Nakharuthai Surintorn Boonanuntanasarn Jirawadee Kaewda Pimpisut Manassila |
| author_facet | Chatsirin Nakharuthai Surintorn Boonanuntanasarn Jirawadee Kaewda Pimpisut Manassila |
| author_sort | Chatsirin Nakharuthai |
| collection | DOAJ |
| description | This study aimed to investigate the potential probiotic <i>Bacillus</i> spp. from the intestine of Nile tilapia in order to construct a recombinant probiotic for the enhancement of the Nile tilapia immune response. One hundred bacterial isolates from the intestine of Nile tilapia were characterized for species identification using the 16s ribosomal RNA (rRNA). Only <i>Bacillus</i> isolates with exhibited antagonistic activity were investigated for their biological functions, which included protease-producing capacity, bile salts and pH tolerance, antibiotic susceptibility, and pathogenicity tests. According to the best results, <i>Bacillus</i> isolate B29, as closely related to <i>B. subtilis</i>, was selected to construct a recombinant probiotic for the delivery of CC chemokine protein (pBES<i>On</i>-CC). The existence of recombinant probiotics was confirmed by Western blotting before the feeding trial. In addition, the CC chemokine mRNA level was quantified in the intestine of fish fed probiotics after 30 days of feeding. Total immunoglobulin, lysozyme activity, alternative complement 50 activity (ACH50), and phagocytic activity of fish fed either wild-type or recombinant probiotics were significantly increased, indicating that probiotics could stimulate the Nile tilapia immune system through different processes. Interestingly, the dietary supplementation of recombinant probiotics has a stronger immune response enhancement than the wild-type strain. |
| first_indexed | 2024-03-11T07:02:41Z |
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| id | doaj.art-8a2bc38221d04b51b0eeefac8f39bf4e |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T07:02:41Z |
| publishDate | 2023-03-01 |
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| series | Animals |
| spelling | doaj.art-8a2bc38221d04b51b0eeefac8f39bf4e2023-11-17T09:10:42ZengMDPI AGAnimals2076-26152023-03-0113698610.3390/ani13060986Isolation of Potential Probiotic <i>Bacillus</i> spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile TilapiaChatsirin Nakharuthai0Surintorn Boonanuntanasarn1Jirawadee Kaewda2Pimpisut Manassila3School of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang, Nakhon Ratchasima 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang, Nakhon Ratchasima 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang, Nakhon Ratchasima 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, 111 University Avenue, Muang, Nakhon Ratchasima 30000, ThailandThis study aimed to investigate the potential probiotic <i>Bacillus</i> spp. from the intestine of Nile tilapia in order to construct a recombinant probiotic for the enhancement of the Nile tilapia immune response. One hundred bacterial isolates from the intestine of Nile tilapia were characterized for species identification using the 16s ribosomal RNA (rRNA). Only <i>Bacillus</i> isolates with exhibited antagonistic activity were investigated for their biological functions, which included protease-producing capacity, bile salts and pH tolerance, antibiotic susceptibility, and pathogenicity tests. According to the best results, <i>Bacillus</i> isolate B29, as closely related to <i>B. subtilis</i>, was selected to construct a recombinant probiotic for the delivery of CC chemokine protein (pBES<i>On</i>-CC). The existence of recombinant probiotics was confirmed by Western blotting before the feeding trial. In addition, the CC chemokine mRNA level was quantified in the intestine of fish fed probiotics after 30 days of feeding. Total immunoglobulin, lysozyme activity, alternative complement 50 activity (ACH50), and phagocytic activity of fish fed either wild-type or recombinant probiotics were significantly increased, indicating that probiotics could stimulate the Nile tilapia immune system through different processes. Interestingly, the dietary supplementation of recombinant probiotics has a stronger immune response enhancement than the wild-type strain.https://www.mdpi.com/2076-2615/13/6/986Nile tilapiaprobioticsCC chemokineimmune responseheterologous protein expression |
| spellingShingle | Chatsirin Nakharuthai Surintorn Boonanuntanasarn Jirawadee Kaewda Pimpisut Manassila Isolation of Potential Probiotic <i>Bacillus</i> spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile Tilapia Animals Nile tilapia probiotics CC chemokine immune response heterologous protein expression |
| title | Isolation of Potential Probiotic <i>Bacillus</i> spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile Tilapia |
| title_full | Isolation of Potential Probiotic <i>Bacillus</i> spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile Tilapia |
| title_fullStr | Isolation of Potential Probiotic <i>Bacillus</i> spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile Tilapia |
| title_full_unstemmed | Isolation of Potential Probiotic <i>Bacillus</i> spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile Tilapia |
| title_short | Isolation of Potential Probiotic <i>Bacillus</i> spp. from the Intestine of Nile Tilapia to Construct Recombinant Probiotic Expressing CC Chemokine and Its Effectiveness on Innate Immune Responses in Nile Tilapia |
| title_sort | isolation of potential probiotic i bacillus i spp from the intestine of nile tilapia to construct recombinant probiotic expressing cc chemokine and its effectiveness on innate immune responses in nile tilapia |
| topic | Nile tilapia probiotics CC chemokine immune response heterologous protein expression |
| url | https://www.mdpi.com/2076-2615/13/6/986 |
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