Skeletal Lipocalin-2 Is Associated with Iron-Related Oxidative Stress in ob/ob Mice with Sarcopenia
Obesity and insulin resistance accelerate aging-related sarcopenia, which is associated with iron load and oxidative stress. Lipocalin-2 (LCN2) is an iron-binding protein that has been associated with skeletal muscle regeneration, but details regarding its role in obese sarcopenia remain unclear. He...
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MDPI AG
2021-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/5/758 |
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| author | Eun Bee Choi Jae Hun Jeong Hye Min Jang Yu Jeong Ahn Kyu Hyeon Kim Hyeong Seok An Jong Youl Lee Eun Ae Jeong Jaewoong Lee Hyun Joo Shin Kyung Eun Kim Gu Seob Roh |
| author_facet | Eun Bee Choi Jae Hun Jeong Hye Min Jang Yu Jeong Ahn Kyu Hyeon Kim Hyeong Seok An Jong Youl Lee Eun Ae Jeong Jaewoong Lee Hyun Joo Shin Kyung Eun Kim Gu Seob Roh |
| author_sort | Eun Bee Choi |
| collection | DOAJ |
| description | Obesity and insulin resistance accelerate aging-related sarcopenia, which is associated with iron load and oxidative stress. Lipocalin-2 (LCN2) is an iron-binding protein that has been associated with skeletal muscle regeneration, but details regarding its role in obese sarcopenia remain unclear. Here, we report that elevated LCN2 levels in skeletal muscle are linked to muscle atrophy-related inflammation and oxidative stress in leptin-deficient ob/ob mice. RNA sequencing analyses indicated the <i>LCN2</i> gene expression is enhanced in skeletal muscle of ob/ob mice with sarcopenia. In addition to muscular iron accumulation in ob/ob mice, expressions of iron homeostasis-related divalent metal transporter 1, ferritin, and hepcidin proteins were increased in ob/ob mice compared to lean littermates, whereas expressions of transferrin receptor and ferroportin were reduced. Collectively, these findings demonstrate that LCN2 functions as a potent proinflammatory factor in skeletal muscle in response to obesity-related sarcopenia and is thus a therapeutic candidate target for sarcopenia treatment. |
| first_indexed | 2024-03-10T11:32:50Z |
| format | Article |
| id | doaj.art-8a2c47e902024a9f8a96d44fdcaa7f0a |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T11:32:50Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-8a2c47e902024a9f8a96d44fdcaa7f0a2023-11-21T19:07:20ZengMDPI AGAntioxidants2076-39212021-05-0110575810.3390/antiox10050758Skeletal Lipocalin-2 Is Associated with Iron-Related Oxidative Stress in ob/ob Mice with SarcopeniaEun Bee Choi0Jae Hun Jeong1Hye Min Jang2Yu Jeong Ahn3Kyu Hyeon Kim4Hyeong Seok An5Jong Youl Lee6Eun Ae Jeong7Jaewoong Lee8Hyun Joo Shin9Kyung Eun Kim10Gu Seob Roh11Department of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaDepartment of Medicine, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaDepartment of Anatomy and Convergence Medical Science, Bio Anti-Aging Medical Research Center, Institute of Health Sciences, College of Medicine, Gyeongsang National University, Jinju 52777, Gyeongnam, KoreaObesity and insulin resistance accelerate aging-related sarcopenia, which is associated with iron load and oxidative stress. Lipocalin-2 (LCN2) is an iron-binding protein that has been associated with skeletal muscle regeneration, but details regarding its role in obese sarcopenia remain unclear. Here, we report that elevated LCN2 levels in skeletal muscle are linked to muscle atrophy-related inflammation and oxidative stress in leptin-deficient ob/ob mice. RNA sequencing analyses indicated the <i>LCN2</i> gene expression is enhanced in skeletal muscle of ob/ob mice with sarcopenia. In addition to muscular iron accumulation in ob/ob mice, expressions of iron homeostasis-related divalent metal transporter 1, ferritin, and hepcidin proteins were increased in ob/ob mice compared to lean littermates, whereas expressions of transferrin receptor and ferroportin were reduced. Collectively, these findings demonstrate that LCN2 functions as a potent proinflammatory factor in skeletal muscle in response to obesity-related sarcopenia and is thus a therapeutic candidate target for sarcopenia treatment.https://www.mdpi.com/2076-3921/10/5/758lipocalin-2ironinflammationoxidative stresssarcopeniaob/ob mouse |
| spellingShingle | Eun Bee Choi Jae Hun Jeong Hye Min Jang Yu Jeong Ahn Kyu Hyeon Kim Hyeong Seok An Jong Youl Lee Eun Ae Jeong Jaewoong Lee Hyun Joo Shin Kyung Eun Kim Gu Seob Roh Skeletal Lipocalin-2 Is Associated with Iron-Related Oxidative Stress in ob/ob Mice with Sarcopenia Antioxidants lipocalin-2 iron inflammation oxidative stress sarcopenia ob/ob mouse |
| title | Skeletal Lipocalin-2 Is Associated with Iron-Related Oxidative Stress in ob/ob Mice with Sarcopenia |
| title_full | Skeletal Lipocalin-2 Is Associated with Iron-Related Oxidative Stress in ob/ob Mice with Sarcopenia |
| title_fullStr | Skeletal Lipocalin-2 Is Associated with Iron-Related Oxidative Stress in ob/ob Mice with Sarcopenia |
| title_full_unstemmed | Skeletal Lipocalin-2 Is Associated with Iron-Related Oxidative Stress in ob/ob Mice with Sarcopenia |
| title_short | Skeletal Lipocalin-2 Is Associated with Iron-Related Oxidative Stress in ob/ob Mice with Sarcopenia |
| title_sort | skeletal lipocalin 2 is associated with iron related oxidative stress in ob ob mice with sarcopenia |
| topic | lipocalin-2 iron inflammation oxidative stress sarcopenia ob/ob mouse |
| url | https://www.mdpi.com/2076-3921/10/5/758 |
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