Gut microbiota and its derived SCFAs regulate the HPGA to reverse obesity-induced precocious puberty in female rats
The intestinal microbiota and its derived short-chain fatty acids (SCFAs) can reverse obesity and obesity-related metabolic diseases, but whether it has an effect on obesity complicated by precocious puberty and its potential mechanism need to be further understood. The purpose of this study was to...
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.1051797/full |
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| author | Li Wang Li Wang Hao Xu Hao Xu Bin Tan Qin Yi Huiwen Liu Hongrong Deng Yunxia Chen Rui Wang Jie Tian Jie Tian Jing Zhu |
| author_facet | Li Wang Li Wang Hao Xu Hao Xu Bin Tan Qin Yi Huiwen Liu Hongrong Deng Yunxia Chen Rui Wang Jie Tian Jie Tian Jing Zhu |
| author_sort | Li Wang |
| collection | DOAJ |
| description | The intestinal microbiota and its derived short-chain fatty acids (SCFAs) can reverse obesity and obesity-related metabolic diseases, but whether it has an effect on obesity complicated by precocious puberty and its potential mechanism need to be further understood. The purpose of this study was to investigate the effect of the gut microbiota and its derived short-chain fatty acids (SCFAs) on obesity-induced precocious puberty rats and their regulatory mechanisms. We constructed obesity-induced precocious puberty rats using a high-fat diet (HFD) had notable similarity to precocious puberty caused by obesity due to overeating in children. We then added acetate, propionate, butyrate or their mixture to the HFD, and investigated the effect of intestinal microbiota and its derived SCFAs on the hypothalamic-pituitary-gonadal axis (HPGA) in rats with obesity-induced precocious puberty. We found that obesity-induced precocious puberty rats had an early first estrous cycle, increased hypothalamic mRNA expression of Kiss1, GPR54 and GnRH, and early gonadal maturation. Meanwhile, the intestinal microbiota imbalance and the main SCFAs production decreased in the colon. The addition of acetate, propionate, butyrate or their mixture to the HFD could significantly reverse the precocious puberty of rats, reduce GnRH release from the hypothalamus and delay the development of the gonadal axis through the Kiss1–GPR54–PKC–ERK1/2 pathway. Our findings suggest that gut microbiota-derived SCFAs are promising therapeutic means for the prevention of obesity-induced precocious puberty and provide new therapeutic strategies with clinical value. |
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| format | Article |
| id | doaj.art-8a2d5f87f38f4d89a7d14c39172be655 |
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| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-04-11T06:17:35Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Endocrinology |
| spelling | doaj.art-8a2d5f87f38f4d89a7d14c39172be6552022-12-22T04:41:01ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-12-011310.3389/fendo.2022.10517971051797Gut microbiota and its derived SCFAs regulate the HPGA to reverse obesity-induced precocious puberty in female ratsLi Wang0Li Wang1Hao Xu2Hao Xu3Bin Tan4Qin Yi5Huiwen Liu6Hongrong Deng7Yunxia Chen8Rui Wang9Jie Tian10Jie Tian11Jing Zhu12Department of Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, ChinaDepartment of Clinical Laboratory, Chengdu Women’s and Children’s Central Hospital, School of Medicine, University of Electronic Science and Technology of China, Chengdu, ChinaDepartment of Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, ChinaDepartment of Clinical Laboratory, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, ChinaDepartment of Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, ChinaDepartment of Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, ChinaDepartment of Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, ChinaDepartment of Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, ChinaDepartment of Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, ChinaDepartment of Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, ChinaDepartment of Cardiovascular Internal Medicine, Children’s Hospital of Chongqing Medical University, Chongqing, ChinaDepartment of Pediatric Research Institute, Children’s Hospital of Chongqing Medical University, National Clinical Research Center for Child Health and Disorders, Ministry of Education Key Laboratory of Child Development and Disorders, Chongqing Key Laboratory of Pediatrics, Chongqing, ChinaThe intestinal microbiota and its derived short-chain fatty acids (SCFAs) can reverse obesity and obesity-related metabolic diseases, but whether it has an effect on obesity complicated by precocious puberty and its potential mechanism need to be further understood. The purpose of this study was to investigate the effect of the gut microbiota and its derived short-chain fatty acids (SCFAs) on obesity-induced precocious puberty rats and their regulatory mechanisms. We constructed obesity-induced precocious puberty rats using a high-fat diet (HFD) had notable similarity to precocious puberty caused by obesity due to overeating in children. We then added acetate, propionate, butyrate or their mixture to the HFD, and investigated the effect of intestinal microbiota and its derived SCFAs on the hypothalamic-pituitary-gonadal axis (HPGA) in rats with obesity-induced precocious puberty. We found that obesity-induced precocious puberty rats had an early first estrous cycle, increased hypothalamic mRNA expression of Kiss1, GPR54 and GnRH, and early gonadal maturation. Meanwhile, the intestinal microbiota imbalance and the main SCFAs production decreased in the colon. The addition of acetate, propionate, butyrate or their mixture to the HFD could significantly reverse the precocious puberty of rats, reduce GnRH release from the hypothalamus and delay the development of the gonadal axis through the Kiss1–GPR54–PKC–ERK1/2 pathway. Our findings suggest that gut microbiota-derived SCFAs are promising therapeutic means for the prevention of obesity-induced precocious puberty and provide new therapeutic strategies with clinical value.https://www.frontiersin.org/articles/10.3389/fendo.2022.1051797/fullobesitypubertymicrobiotashort chain fatty acids (SCFAs)high-fat diet |
| spellingShingle | Li Wang Li Wang Hao Xu Hao Xu Bin Tan Qin Yi Huiwen Liu Hongrong Deng Yunxia Chen Rui Wang Jie Tian Jie Tian Jing Zhu Gut microbiota and its derived SCFAs regulate the HPGA to reverse obesity-induced precocious puberty in female rats Frontiers in Endocrinology obesity puberty microbiota short chain fatty acids (SCFAs) high-fat diet |
| title | Gut microbiota and its derived SCFAs regulate the HPGA to reverse obesity-induced precocious puberty in female rats |
| title_full | Gut microbiota and its derived SCFAs regulate the HPGA to reverse obesity-induced precocious puberty in female rats |
| title_fullStr | Gut microbiota and its derived SCFAs regulate the HPGA to reverse obesity-induced precocious puberty in female rats |
| title_full_unstemmed | Gut microbiota and its derived SCFAs regulate the HPGA to reverse obesity-induced precocious puberty in female rats |
| title_short | Gut microbiota and its derived SCFAs regulate the HPGA to reverse obesity-induced precocious puberty in female rats |
| title_sort | gut microbiota and its derived scfas regulate the hpga to reverse obesity induced precocious puberty in female rats |
| topic | obesity puberty microbiota short chain fatty acids (SCFAs) high-fat diet |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.1051797/full |
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