Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-κB and restoring lipid production in Caco-2 cell line
Paenibacillus polymyxa exopolysaccharide PYQ1-EPS is a homogeneous glucomannan-type polysaccharide. In this work, its stability and reparative effects on the in vitro model of inflammatory bowel disease (IBD) and possible mechanisms were investigated to expand its application fields. The results sho...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464623003092 |
| _version_ | 1797749116815540224 |
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| author | Jing Zhang Zhi-Qiang Li Ru-Jing Zheng Jin-Jie Jiang Dan-Dan Li Wen-Wen Zhou |
| author_facet | Jing Zhang Zhi-Qiang Li Ru-Jing Zheng Jin-Jie Jiang Dan-Dan Li Wen-Wen Zhou |
| author_sort | Jing Zhang |
| collection | DOAJ |
| description | Paenibacillus polymyxa exopolysaccharide PYQ1-EPS is a homogeneous glucomannan-type polysaccharide. In this work, its stability and reparative effects on the in vitro model of inflammatory bowel disease (IBD) and possible mechanisms were investigated to expand its application fields. The results showed that PYQ1-EPS significantly increased the cell viability, inhibited the expression of pro-inflammatory cytokines and promoted the expression of anti-inflammatory cytokines, which effectively blocked the activation of NF-κB and MAPK signaling pathways. Meanwhile, it was found that the anti-inflammatory effect of PYQ1-EPS was closely related to the lipid metabolism of cells, which restored the lipid content of inflammation model cells to 90.7% of the untreated cells. This is the first report that bacterial polysaccharides inhibit gastrointestinal inflammation by modulating lipid metabolism. Upon the understanding of its functional mechanisms, our work demonstrated that PYQ1-EPS has great potential to be developed as a functional food for gastrointestinal protection. |
| first_indexed | 2024-03-12T16:15:36Z |
| format | Article |
| id | doaj.art-8a32f5209e0b49ac9ba57e0b0c707f5c |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-03-12T16:15:36Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-8a32f5209e0b49ac9ba57e0b0c707f5c2023-08-09T04:32:40ZengElsevierJournal of Functional Foods1756-46462023-08-01107105709Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-κB and restoring lipid production in Caco-2 cell lineJing Zhang0Zhi-Qiang Li1Ru-Jing Zheng2Jin-Jie Jiang3Dan-Dan Li4Wen-Wen Zhou5Institute of Food Bioscience and Technology, College of Biosystems Engineering and Food Science, Ningbo Research Institute, Zhejiang University, Hangzhou 310058, Zhejiang, China; School of Chemical and Biomolecular Engineering, The University of Sydney, NSW 2006, AustraliaInstitute of Food Bioscience and Technology, College of Biosystems Engineering and Food Science, Ningbo Research Institute, Zhejiang University, Hangzhou 310058, Zhejiang, ChinaZhejiang Homay Technology Co., Ltd, Hangzhou 311200, Zhejiang, ChinaZhejiang Homay Technology Co., Ltd, Hangzhou 311200, Zhejiang, ChinaZhejiang Homay Technology Co., Ltd, Hangzhou 311200, Zhejiang, ChinaInstitute of Food Bioscience and Technology, College of Biosystems Engineering and Food Science, Ningbo Research Institute, Zhejiang University, Hangzhou 310058, Zhejiang, China; Corresponding author.Paenibacillus polymyxa exopolysaccharide PYQ1-EPS is a homogeneous glucomannan-type polysaccharide. In this work, its stability and reparative effects on the in vitro model of inflammatory bowel disease (IBD) and possible mechanisms were investigated to expand its application fields. The results showed that PYQ1-EPS significantly increased the cell viability, inhibited the expression of pro-inflammatory cytokines and promoted the expression of anti-inflammatory cytokines, which effectively blocked the activation of NF-κB and MAPK signaling pathways. Meanwhile, it was found that the anti-inflammatory effect of PYQ1-EPS was closely related to the lipid metabolism of cells, which restored the lipid content of inflammation model cells to 90.7% of the untreated cells. This is the first report that bacterial polysaccharides inhibit gastrointestinal inflammation by modulating lipid metabolism. Upon the understanding of its functional mechanisms, our work demonstrated that PYQ1-EPS has great potential to be developed as a functional food for gastrointestinal protection.http://www.sciencedirect.com/science/article/pii/S1756464623003092Exopolysaccharide stabilityGastrointestinal inflammation repairLipid mechanism |
| spellingShingle | Jing Zhang Zhi-Qiang Li Ru-Jing Zheng Jin-Jie Jiang Dan-Dan Li Wen-Wen Zhou Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-κB and restoring lipid production in Caco-2 cell line Journal of Functional Foods Exopolysaccharide stability Gastrointestinal inflammation repair Lipid mechanism |
| title | Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-κB and restoring lipid production in Caco-2 cell line |
| title_full | Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-κB and restoring lipid production in Caco-2 cell line |
| title_fullStr | Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-κB and restoring lipid production in Caco-2 cell line |
| title_full_unstemmed | Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-κB and restoring lipid production in Caco-2 cell line |
| title_short | Paenibacillus exopolysaccharide repairs GI inflammation by suppressing MAPK and NF-κB and restoring lipid production in Caco-2 cell line |
| title_sort | paenibacillus exopolysaccharide repairs gi inflammation by suppressing mapk and nf κb and restoring lipid production in caco 2 cell line |
| topic | Exopolysaccharide stability Gastrointestinal inflammation repair Lipid mechanism |
| url | http://www.sciencedirect.com/science/article/pii/S1756464623003092 |
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