Utilization of Lignin Monomer Isolation from Black Liquor of Empty Palm Oil Bunches as a Prebiotic
Waste is generated in the form of black liquor during pulp processing. Furthermore, lignin, the main ingredient of black liquor, contains a phenylpropanoid compound with prebiotic and antimicrobial activity. Also, it is a component of lignocellulose that has prebiotic and antimicrobial activity effe...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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University of Zagreb, Faculty of Forestry and Wood Technology
2023-01-01
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| Series: | Drvna Industrija |
| Subjects: | |
| Online Access: | https://hrcak.srce.hr/file/428822 |
| _version_ | 1797847915310350336 |
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| author | Sri Hidayati Subeki Subeki M. Perdiansyah Mulia Harahap Sutopo Hadi |
| author_facet | Sri Hidayati Subeki Subeki M. Perdiansyah Mulia Harahap Sutopo Hadi |
| author_sort | Sri Hidayati |
| collection | DOAJ |
| description | Waste is generated in the form of black liquor during pulp processing. Furthermore, lignin, the main ingredient of black liquor, contains a phenylpropanoid compound with prebiotic and antimicrobial activity. Also, it is a component of lignocellulose that has prebiotic and antimicrobial activity effects due to its indigestible nature and it comprises phenylpropanoid components. Therefore, this study examines and identifies the lignin purification and test results of its prebiotic activity. The technique used to identify lignin fraction is called Thin Layer Chromatography (TLC) and Gas Chromatography-Mass Spectrometry (GC-MS) plates. Prebiotic activity tests were performed using the calculation of total bacteria on the growth of Lactobacillus casei. The results showed that the purification process using CHCl3, 3 % MeOH:CHCl3, 20 % MeOH:CHCl3, and MeOH yielded 10.68 %, 6.34 %, 11.38 % 44.85 %, respectively. The 3 % MeOH:CHCl3 fraction contained benzaldehyde, 4- hydroxy-3,5- dimethoxy, 1-methylbutyl hexadecanoate, oleic acid, and di-2-ethylhexyl phthalate. The 3 % MeOH:CHCl3 fractionate with a concentration of 15 % also showed a prebiotic activity for L. casei at 6.1 x 102 colonies/mL. |
| first_indexed | 2024-04-09T18:18:59Z |
| format | Article |
| id | doaj.art-8a36065f633f4308a40f74a645d44979 |
| institution | Directory Open Access Journal |
| issn | 0012-6772 1847-1153 |
| language | English |
| last_indexed | 2024-04-09T18:18:59Z |
| publishDate | 2023-01-01 |
| publisher | University of Zagreb, Faculty of Forestry and Wood Technology |
| record_format | Article |
| series | Drvna Industrija |
| spelling | doaj.art-8a36065f633f4308a40f74a645d449792023-04-12T15:28:53ZengUniversity of Zagreb, Faculty of Forestry and Wood TechnologyDrvna Industrija0012-67721847-11532023-01-01741536010.5552/drvind.2023.001515Utilization of Lignin Monomer Isolation from Black Liquor of Empty Palm Oil Bunches as a PrebioticSri Hidayati0Subeki Subeki1M. Perdiansyah Mulia Harahap2Sutopo Hadi3Lampung University, Agricultural Technology Department, Bandar Lampung, IndonesiaLampung University, Agricultural Technology Department, Bandar Lampung, IndonesiaLampung State Polytechnic, Bandar Lampung, IndonesiaLampung University, Department of Chemistry, Bandar Lampung, IndonesiaWaste is generated in the form of black liquor during pulp processing. Furthermore, lignin, the main ingredient of black liquor, contains a phenylpropanoid compound with prebiotic and antimicrobial activity. Also, it is a component of lignocellulose that has prebiotic and antimicrobial activity effects due to its indigestible nature and it comprises phenylpropanoid components. Therefore, this study examines and identifies the lignin purification and test results of its prebiotic activity. The technique used to identify lignin fraction is called Thin Layer Chromatography (TLC) and Gas Chromatography-Mass Spectrometry (GC-MS) plates. Prebiotic activity tests were performed using the calculation of total bacteria on the growth of Lactobacillus casei. The results showed that the purification process using CHCl3, 3 % MeOH:CHCl3, 20 % MeOH:CHCl3, and MeOH yielded 10.68 %, 6.34 %, 11.38 % 44.85 %, respectively. The 3 % MeOH:CHCl3 fraction contained benzaldehyde, 4- hydroxy-3,5- dimethoxy, 1-methylbutyl hexadecanoate, oleic acid, and di-2-ethylhexyl phthalate. The 3 % MeOH:CHCl3 fractionate with a concentration of 15 % also showed a prebiotic activity for L. casei at 6.1 x 102 colonies/mL.https://hrcak.srce.hr/file/428822black liquorlignin monomerempty palm oil bunchprebiotic |
| spellingShingle | Sri Hidayati Subeki Subeki M. Perdiansyah Mulia Harahap Sutopo Hadi Utilization of Lignin Monomer Isolation from Black Liquor of Empty Palm Oil Bunches as a Prebiotic Drvna Industrija black liquor lignin monomer empty palm oil bunch prebiotic |
| title | Utilization of Lignin Monomer Isolation from Black Liquor of Empty Palm Oil Bunches as a Prebiotic |
| title_full | Utilization of Lignin Monomer Isolation from Black Liquor of Empty Palm Oil Bunches as a Prebiotic |
| title_fullStr | Utilization of Lignin Monomer Isolation from Black Liquor of Empty Palm Oil Bunches as a Prebiotic |
| title_full_unstemmed | Utilization of Lignin Monomer Isolation from Black Liquor of Empty Palm Oil Bunches as a Prebiotic |
| title_short | Utilization of Lignin Monomer Isolation from Black Liquor of Empty Palm Oil Bunches as a Prebiotic |
| title_sort | utilization of lignin monomer isolation from black liquor of empty palm oil bunches as a prebiotic |
| topic | black liquor lignin monomer empty palm oil bunch prebiotic |
| url | https://hrcak.srce.hr/file/428822 |
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