Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells
Background: Among the most important factors in wound healing pathways are transforming growth factor beta1 and vascular endothelial growth factor. Fibroblasts are the main cell in all phases wound closure. In this study, the extracts of plant materials such as Adiantum capillus-veneris, Commiphora...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2017-01-01
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| Series: | International Journal of Preventive Medicine |
| Subjects: | |
| Online Access: | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2017;volume=8;issue=1;spage=18;epage=18;aulast= |
| _version_ | 1818983345426530304 |
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| author | Samira Negahdari Hamid Galehdari Mahnaz Kesmati Anahita Rezaie Gholamreza Shariati |
| author_facet | Samira Negahdari Hamid Galehdari Mahnaz Kesmati Anahita Rezaie Gholamreza Shariati |
| author_sort | Samira Negahdari |
| collection | DOAJ |
| description | Background: Among the most important factors in wound healing pathways are transforming growth factor beta1 and vascular endothelial growth factor. Fibroblasts are the main cell in all phases wound closure. In this study, the extracts of plant materials such as Adiantum capillus-veneris, Commiphora molmol, Aloe vera, and henna and one mixture of them were used to treatment of normal mouse skin fibroblasts.
Methods: Cytotoxic effects of each extract and their mixture were assessed on mouse skin fibroblasts cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We performed migration assays to assess migration properties of mouse skin fibroblasts cells in response to the extracts. Changes in the gene expression of the Tgf β 1and Vegf-A genes were monitored by real-time polymerase chain reaction.
Results: A. capillus-veneris, C. molmol and henna extract improved the expression of Tgf β 1 gene. All used extracts upregulated the expression of Vegf-A gene and promoted the migration of mouse fibroblast cells in vitro.
Conclusions: The present study demonstrated that the mentioned herbal extracts might be effective in wound healing, through the improvement in the migration of fibroblast cells and regulating the gene expression of Tgf β 1 and Vegf-A genes in fibroblast cells treated with extracts. |
| first_indexed | 2024-12-20T18:01:38Z |
| format | Article |
| id | doaj.art-8a38721a7e02454cafee521e0f53dbee |
| institution | Directory Open Access Journal |
| issn | 2008-7802 2008-8213 |
| language | English |
| last_indexed | 2024-12-20T18:01:38Z |
| publishDate | 2017-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | International Journal of Preventive Medicine |
| spelling | doaj.art-8a38721a7e02454cafee521e0f53dbee2022-12-21T19:30:37ZengWolters Kluwer Medknow PublicationsInternational Journal of Preventive Medicine2008-78022008-82132017-01-0181181810.4103/ijpvm.IJPVM_338_16Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cellsSamira NegahdariHamid GalehdariMahnaz KesmatiAnahita RezaieGholamreza ShariatiBackground: Among the most important factors in wound healing pathways are transforming growth factor beta1 and vascular endothelial growth factor. Fibroblasts are the main cell in all phases wound closure. In this study, the extracts of plant materials such as Adiantum capillus-veneris, Commiphora molmol, Aloe vera, and henna and one mixture of them were used to treatment of normal mouse skin fibroblasts. Methods: Cytotoxic effects of each extract and their mixture were assessed on mouse skin fibroblasts cells using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. We performed migration assays to assess migration properties of mouse skin fibroblasts cells in response to the extracts. Changes in the gene expression of the Tgf β 1and Vegf-A genes were monitored by real-time polymerase chain reaction. Results: A. capillus-veneris, C. molmol and henna extract improved the expression of Tgf β 1 gene. All used extracts upregulated the expression of Vegf-A gene and promoted the migration of mouse fibroblast cells in vitro. Conclusions: The present study demonstrated that the mentioned herbal extracts might be effective in wound healing, through the improvement in the migration of fibroblast cells and regulating the gene expression of Tgf β 1 and Vegf-A genes in fibroblast cells treated with extracts.http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2017;volume=8;issue=1;spage=18;epage=18;aulast=Fibroblastherbaltransforming growth factor-β1vascular endothelial growth factorwound healing |
| spellingShingle | Samira Negahdari Hamid Galehdari Mahnaz Kesmati Anahita Rezaie Gholamreza Shariati Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells International Journal of Preventive Medicine Fibroblast herbal transforming growth factor-β1 vascular endothelial growth factor wound healing |
| title | Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells |
| title_full | Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells |
| title_fullStr | Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells |
| title_full_unstemmed | Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells |
| title_short | Wound healing activity of extracts and formulations of aloe vera, henna, adiantum capillus-veneris, and myrrh on mouse dermal fibroblast cells |
| title_sort | wound healing activity of extracts and formulations of aloe vera henna adiantum capillus veneris and myrrh on mouse dermal fibroblast cells |
| topic | Fibroblast herbal transforming growth factor-β1 vascular endothelial growth factor wound healing |
| url | http://www.ijpvmjournal.net/article.asp?issn=2008-7802;year=2017;volume=8;issue=1;spage=18;epage=18;aulast= |
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