The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent Berenil
The in situ DNA-damaging capacity of berenil (1) has been investigated usingan electrochemical approach employing double stranded (ds) DNA-modified glassy carbonelectrode biosensors. Electrochemical voltammetric sensing of damage caused by 1 todsDNA was monitored by the appearance of peaks diagnosti...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2008-03-01
|
| Series: | Sensors |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1424-8220/8/3/1519/ |
| _version_ | 1798041075744505856 |
|---|---|
| author | MarÃÂlia O. F. Goulart Carlos E. Salas Marcelo M. Santoro Alexandre M. C. Santos Julio C. D. Lopes Valberes B. Nascimento Danielle C. M. Ferreira Francine S. de Paula Fabiane C. de Abreu |
| author_facet | MarÃÂlia O. F. Goulart Carlos E. Salas Marcelo M. Santoro Alexandre M. C. Santos Julio C. D. Lopes Valberes B. Nascimento Danielle C. M. Ferreira Francine S. de Paula Fabiane C. de Abreu |
| author_sort | MarÃÂlia O. F. Goulart |
| collection | DOAJ |
| description | The in situ DNA-damaging capacity of berenil (1) has been investigated usingan electrochemical approach employing double stranded (ds) DNA-modified glassy carbonelectrode biosensors. Electrochemical voltammetric sensing of damage caused by 1 todsDNA was monitored by the appearance of peaks diagnostic of the oxidation of guanineand adenine. When 1 was incorporated directly onto the biosensor surface, DNA damagecould be observed at concentrations of additive as low as 10 μM. In contrast, when thedsDNA-modified biosensor was exposed to 1, in acetate buffer solution, the method wasmuch less sensitive and DNA damage could be detected only in the presence of 100 μMberenil. When mixed solutions of 1 and single stranded (ss) DNA, polyguanylic acid orpolyadenylic acid were submitted to voltammetric study, the oxidation signals of therespective bases decreased in a concentration-dependent manner and the major variation ofthe adenine current peak indicated preferential binding of 1 to adenine. The electrochemical results were in close agreement with those deriving from a differentialscanning calorimetric study of the DNA-berenil complex. |
| first_indexed | 2024-04-11T22:17:25Z |
| format | Article |
| id | doaj.art-8a3908446396441eb2f9abd249cc9815 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T22:17:25Z |
| publishDate | 2008-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-8a3908446396441eb2f9abd249cc98152022-12-22T04:00:21ZengMDPI AGSensors1424-82202008-03-018315191538The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent BerenilMarÃÂlia O. F. GoulartCarlos E. SalasMarcelo M. SantoroAlexandre M. C. SantosJulio C. D. LopesValberes B. NascimentoDanielle C. M. FerreiraFrancine S. de PaulaFabiane C. de AbreuThe in situ DNA-damaging capacity of berenil (1) has been investigated usingan electrochemical approach employing double stranded (ds) DNA-modified glassy carbonelectrode biosensors. Electrochemical voltammetric sensing of damage caused by 1 todsDNA was monitored by the appearance of peaks diagnostic of the oxidation of guanineand adenine. When 1 was incorporated directly onto the biosensor surface, DNA damagecould be observed at concentrations of additive as low as 10 μM. In contrast, when thedsDNA-modified biosensor was exposed to 1, in acetate buffer solution, the method wasmuch less sensitive and DNA damage could be detected only in the presence of 100 μMberenil. When mixed solutions of 1 and single stranded (ss) DNA, polyguanylic acid orpolyadenylic acid were submitted to voltammetric study, the oxidation signals of therespective bases decreased in a concentration-dependent manner and the major variation ofthe adenine current peak indicated preferential binding of 1 to adenine. The electrochemical results were in close agreement with those deriving from a differentialscanning calorimetric study of the DNA-berenil complex.http://www.mdpi.com/1424-8220/8/3/1519/BerenildsDNA biosensorElectrochemical voltammetric sensingDNA damageDifferential scanning calorimetry |
| spellingShingle | MarÃÂlia O. F. Goulart Carlos E. Salas Marcelo M. Santoro Alexandre M. C. Santos Julio C. D. Lopes Valberes B. Nascimento Danielle C. M. Ferreira Francine S. de Paula Fabiane C. de Abreu The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent Berenil Sensors Berenil dsDNA biosensor Electrochemical voltammetric sensing DNA damage Differential scanning calorimetry |
| title | The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent Berenil |
| title_full | The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent Berenil |
| title_fullStr | The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent Berenil |
| title_full_unstemmed | The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent Berenil |
| title_short | The Application of DNA-Biosensors and Differential Scanning Calorimetry to the Study of the DNA-Binding Agent Berenil |
| title_sort | application of dna biosensors and differential scanning calorimetry to the study of the dna binding agent berenil |
| topic | Berenil dsDNA biosensor Electrochemical voltammetric sensing DNA damage Differential scanning calorimetry |
| url | http://www.mdpi.com/1424-8220/8/3/1519/ |
| work_keys_str_mv | AT maraƒaliaofgoulart theapplicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT carlosesalas theapplicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT marcelomsantoro theapplicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT alexandremcsantos theapplicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT juliocdlopes theapplicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT valberesbnascimento theapplicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT daniellecmferreira theapplicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT francinesdepaula theapplicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT fabianecdeabreu theapplicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT maraƒaliaofgoulart applicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT carlosesalas applicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT marcelomsantoro applicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT alexandremcsantos applicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT juliocdlopes applicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT valberesbnascimento applicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT daniellecmferreira applicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT francinesdepaula applicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil AT fabianecdeabreu applicationofdnabiosensorsanddifferentialscanningcalorimetrytothestudyofthednabindingagentberenil |