Comparison of Multiple Bioactive Constituents in the Flower and the Caulis of <i>Lonicera japonica</i> Based on UFLC-QTRAP-MS/MS Combined with Multivariate Statistical Analysis
Lonicerae japonicae flos (LJF) and Lonicerae japonicae caulis (LJC) are derived from different parts of <i>Lonicera japonica</i> Thunb. (Caprifoliaceae), and have been used as herbal remedies to treat various diseases for thousands of years with confirmed curative effects. However, littl...
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MDPI AG
2019-05-01
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| Series: | Molecules |
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| author | Zhichen Cai Chengcheng Wang Lisi Zou Xunhong Liu Jiali Chen Mengxia Tan Yuqi Mei Lifang Wei |
| author_facet | Zhichen Cai Chengcheng Wang Lisi Zou Xunhong Liu Jiali Chen Mengxia Tan Yuqi Mei Lifang Wei |
| author_sort | Zhichen Cai |
| collection | DOAJ |
| description | Lonicerae japonicae flos (LJF) and Lonicerae japonicae caulis (LJC) are derived from different parts of <i>Lonicera japonica</i> Thunb. (Caprifoliaceae), and have been used as herbal remedies to treat various diseases for thousands of years with confirmed curative effects. However, little attention has been paid to illustrating the differences in efficacy from the perspective of phytochemistry. In the present study, a simultaneous determination of 47 bioactive constituents, including 12 organic acids, 12 flavonoids, six iridoids, 13 amino acids and four nucleosides in 44 batches of LJF and LJC samples from different habitats and commercial herbs was established based on ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry (UFLC-QTRAP-MS/MS). Moreover, principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and <i>t</i>-test were then performed to classify and reveal the differential compositions of LJF and LJC according to the content of the tested constituents. The results demonstrated that the types and contents of chemical components (e.g., isochlorogenic acid A, chlorogenic acid, neochlorogenic acid, quinic acid, secologanic acid, luteoloside, loganin, secoxyloganin, morroniside and L-isoleucine) were significantly different, which may lead to the classification and the differences in efficacy of LJF and LJC. Our findings not only provide a basis for the comprehensive evaluation and intrinsic quality control of LJF and LJC, but also pave the way for discovering the material basis contributing to the different properties and efficacies of the two medicinal materials at the phytochemical level. |
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| publishDate | 2019-05-01 |
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| spelling | doaj.art-8a3ae0e3281848f1b15849ba4b7c14642022-12-22T01:23:10ZengMDPI AGMolecules1420-30492019-05-012410193610.3390/molecules24101936molecules24101936Comparison of Multiple Bioactive Constituents in the Flower and the Caulis of <i>Lonicera japonica</i> Based on UFLC-QTRAP-MS/MS Combined with Multivariate Statistical AnalysisZhichen Cai0Chengcheng Wang1Lisi Zou2Xunhong Liu3Jiali Chen4Mengxia Tan5Yuqi Mei6Lifang Wei7College of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaCollege of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, ChinaLonicerae japonicae flos (LJF) and Lonicerae japonicae caulis (LJC) are derived from different parts of <i>Lonicera japonica</i> Thunb. (Caprifoliaceae), and have been used as herbal remedies to treat various diseases for thousands of years with confirmed curative effects. However, little attention has been paid to illustrating the differences in efficacy from the perspective of phytochemistry. In the present study, a simultaneous determination of 47 bioactive constituents, including 12 organic acids, 12 flavonoids, six iridoids, 13 amino acids and four nucleosides in 44 batches of LJF and LJC samples from different habitats and commercial herbs was established based on ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry (UFLC-QTRAP-MS/MS). Moreover, principal component analysis (PCA), partial least squares discriminant analysis (PLS-DA) and <i>t</i>-test were then performed to classify and reveal the differential compositions of LJF and LJC according to the content of the tested constituents. The results demonstrated that the types and contents of chemical components (e.g., isochlorogenic acid A, chlorogenic acid, neochlorogenic acid, quinic acid, secologanic acid, luteoloside, loganin, secoxyloganin, morroniside and L-isoleucine) were significantly different, which may lead to the classification and the differences in efficacy of LJF and LJC. Our findings not only provide a basis for the comprehensive evaluation and intrinsic quality control of LJF and LJC, but also pave the way for discovering the material basis contributing to the different properties and efficacies of the two medicinal materials at the phytochemical level.https://www.mdpi.com/1420-3049/24/10/1936Lonicerae japonicae flosLonicerae japonicae caulisUFLC-QTRAP-MS/MSbioactive constituentsmultivariate statistical analysis |
| spellingShingle | Zhichen Cai Chengcheng Wang Lisi Zou Xunhong Liu Jiali Chen Mengxia Tan Yuqi Mei Lifang Wei Comparison of Multiple Bioactive Constituents in the Flower and the Caulis of <i>Lonicera japonica</i> Based on UFLC-QTRAP-MS/MS Combined with Multivariate Statistical Analysis Molecules Lonicerae japonicae flos Lonicerae japonicae caulis UFLC-QTRAP-MS/MS bioactive constituents multivariate statistical analysis |
| title | Comparison of Multiple Bioactive Constituents in the Flower and the Caulis of <i>Lonicera japonica</i> Based on UFLC-QTRAP-MS/MS Combined with Multivariate Statistical Analysis |
| title_full | Comparison of Multiple Bioactive Constituents in the Flower and the Caulis of <i>Lonicera japonica</i> Based on UFLC-QTRAP-MS/MS Combined with Multivariate Statistical Analysis |
| title_fullStr | Comparison of Multiple Bioactive Constituents in the Flower and the Caulis of <i>Lonicera japonica</i> Based on UFLC-QTRAP-MS/MS Combined with Multivariate Statistical Analysis |
| title_full_unstemmed | Comparison of Multiple Bioactive Constituents in the Flower and the Caulis of <i>Lonicera japonica</i> Based on UFLC-QTRAP-MS/MS Combined with Multivariate Statistical Analysis |
| title_short | Comparison of Multiple Bioactive Constituents in the Flower and the Caulis of <i>Lonicera japonica</i> Based on UFLC-QTRAP-MS/MS Combined with Multivariate Statistical Analysis |
| title_sort | comparison of multiple bioactive constituents in the flower and the caulis of i lonicera japonica i based on uflc qtrap ms ms combined with multivariate statistical analysis |
| topic | Lonicerae japonicae flos Lonicerae japonicae caulis UFLC-QTRAP-MS/MS bioactive constituents multivariate statistical analysis |
| url | https://www.mdpi.com/1420-3049/24/10/1936 |
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