Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ)
ABSTRACTUpwelling systems contain a high diversity of pelagic microorganisms and their composition and activity are defined by factors like temperature and nutrient concentration. Denaturing gradient gel electrophoresis (DGGE) technique was used to verify the spatial and temporal genetic variability...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2015-09-01
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| Series: | Anais da Academia Brasileira de Ciências |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401737&lng=en&tlng=en |
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| author | SERGIO A. COELHO-SOUZA FÁBIO V. ARAÚJO JULIANO C. CURY HUGO E. JESUS GILBERTO C. PEREIRA JEAN R.D. GUIMARÃES RAQUEL S. PEIXOTO ALBERTO M.R. DÁVILA ALEXANDRE S. ROSADO |
| author_facet | SERGIO A. COELHO-SOUZA FÁBIO V. ARAÚJO JULIANO C. CURY HUGO E. JESUS GILBERTO C. PEREIRA JEAN R.D. GUIMARÃES RAQUEL S. PEIXOTO ALBERTO M.R. DÁVILA ALEXANDRE S. ROSADO |
| author_sort | SERGIO A. COELHO-SOUZA |
| collection | DOAJ |
| description | ABSTRACTUpwelling systems contain a high diversity of pelagic microorganisms and their composition and activity are defined by factors like temperature and nutrient concentration. Denaturing gradient gel electrophoresis (DGGE) technique was used to verify the spatial and temporal genetic variability of Bacteria and Archaea in two stations of the Arraial do Cabo coastal region, one under upwelling pressure and another under anthropogenic pressure. In addition, biotic and abiotic variables were measured in surface and deep waters from three other stations between these stations. Six samplings were done during a year and adequately represented the degrees of upwelling and anthropogenic pressures to the system. Principal Component Analysis (PCA) showed negative correlations between the concentrations of ammonia and phosphorous with prokaryotic secondary production and the total heterotrophic bacteria. PCA also showed negative correlation between temperature and the abundance of prokaryotic cells. Bacterial and archaeal compositions were changeable as were the oceanographic conditions, and upwelling had a regional pressure while anthropogenic pressure was punctual. We suggest that the measurement of prokaryotic secondary production was associated with both Bacteria and Archaea activities, and that substrate availability and temperature determine nutrients cycling. |
| first_indexed | 2024-12-12T14:12:04Z |
| format | Article |
| id | doaj.art-8a44e2f599974809b23257026aa8d711 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-12-12T14:12:04Z |
| publishDate | 2015-09-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-8a44e2f599974809b23257026aa8d7112022-12-22T00:22:04ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902015-09-018731737175010.1590/0001-3765201520140098S0001-37652015000401737Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ)SERGIO A. COELHO-SOUZAFÁBIO V. ARAÚJOJULIANO C. CURYHUGO E. JESUSGILBERTO C. PEREIRAJEAN R.D. GUIMARÃESRAQUEL S. PEIXOTOALBERTO M.R. DÁVILAALEXANDRE S. ROSADOABSTRACTUpwelling systems contain a high diversity of pelagic microorganisms and their composition and activity are defined by factors like temperature and nutrient concentration. Denaturing gradient gel electrophoresis (DGGE) technique was used to verify the spatial and temporal genetic variability of Bacteria and Archaea in two stations of the Arraial do Cabo coastal region, one under upwelling pressure and another under anthropogenic pressure. In addition, biotic and abiotic variables were measured in surface and deep waters from three other stations between these stations. Six samplings were done during a year and adequately represented the degrees of upwelling and anthropogenic pressures to the system. Principal Component Analysis (PCA) showed negative correlations between the concentrations of ammonia and phosphorous with prokaryotic secondary production and the total heterotrophic bacteria. PCA also showed negative correlation between temperature and the abundance of prokaryotic cells. Bacterial and archaeal compositions were changeable as were the oceanographic conditions, and upwelling had a regional pressure while anthropogenic pressure was punctual. We suggest that the measurement of prokaryotic secondary production was associated with both Bacteria and Archaea activities, and that substrate availability and temperature determine nutrients cycling.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401737&lng=en&tlng=endiversidade microbianaincorporação de leucinacitometria de fluxotemperaturadisponibilidade de nutrientesSistema Brasileiro de Ressurgência |
| spellingShingle | SERGIO A. COELHO-SOUZA FÁBIO V. ARAÚJO JULIANO C. CURY HUGO E. JESUS GILBERTO C. PEREIRA JEAN R.D. GUIMARÃES RAQUEL S. PEIXOTO ALBERTO M.R. DÁVILA ALEXANDRE S. ROSADO Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ) Anais da Academia Brasileira de Ciências diversidade microbiana incorporação de leucina citometria de fluxo temperatura disponibilidade de nutrientes Sistema Brasileiro de Ressurgência |
| title | Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ) |
| title_full | Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ) |
| title_fullStr | Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ) |
| title_full_unstemmed | Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ) |
| title_short | Bacterial and Archaeal Communities Variability Associated with Upwelling and Anthropogenic Pressures in the Protection Area of Arraial do Cabo (Cabo Frio region - RJ) |
| title_sort | bacterial and archaeal communities variability associated with upwelling and anthropogenic pressures in the protection area of arraial do cabo cabo frio region rj |
| topic | diversidade microbiana incorporação de leucina citometria de fluxo temperatura disponibilidade de nutrientes Sistema Brasileiro de Ressurgência |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000401737&lng=en&tlng=en |
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