Absolute Quantitative Volatile Measurement from Fresh Tea Leaves and the Derived Teas Revealed Contributions of Postharvest Synthesis of Endogenous Volatiles for the Aroma Quality of Made Teas

Characteristic aroma is a well-appreciated feature contributing to tea quality. Although extensive studies have been made to investigate aroma biosynthesis and gene expressions during tea making processes, it remains unclear whether the endogenous volatile biosynthesis during postharvest tea process...

Full description

Bibliographic Details
Main Authors: Mingjie Chen, Li Guo, Huiwen Zhou, Yaling Guo, Yi Zhang, Zhi Lin, Meng Sun, Wei Zeng, Hualing Wu
Format: Article
Language:English
Published: MDPI AG 2021-01-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/11/2/613
_version_ 1827602569488236544
author Mingjie Chen
Li Guo
Huiwen Zhou
Yaling Guo
Yi Zhang
Zhi Lin
Meng Sun
Wei Zeng
Hualing Wu
author_facet Mingjie Chen
Li Guo
Huiwen Zhou
Yaling Guo
Yi Zhang
Zhi Lin
Meng Sun
Wei Zeng
Hualing Wu
author_sort Mingjie Chen
collection DOAJ
description Characteristic aroma is a well-appreciated feature contributing to tea quality. Although extensive studies have been made to investigate aroma biosynthesis and gene expressions during tea making processes, it remains unclear whether the endogenous volatile biosynthesis during postharvest tea processing contributes to the aroma quality of made tea. To critically evaluate this question, we used the same batch of fresh tea leaves and produced three different types of tea with different degrees of fermentation (green tea, oolong tea, and black tea). Total volatiles were extracted by solvent-assisted-flavor evaporation, then quantified by gas chromatography-flame ionization detector combined with response factor correction for quantitative measurement. Compared with fresh tea leaves, the volatile profiles of the made teas were dramatically altered, with significant loss for the majority of endogenous volatiles and simultaneous gain for non-endogenous volatiles. By calculation of odor-activity values, the potential volatiles contributing to the aroma characteristics of each tea type were identified. Our data suggest that postharvest synthesis of endogenous volatiles did not contribute to the aroma quality of made tea.
first_indexed 2024-03-09T05:20:15Z
format Article
id doaj.art-8a4b521264d2493483eacc919e1c570e
institution Directory Open Access Journal
issn 2076-3417
language English
last_indexed 2024-03-09T05:20:15Z
publishDate 2021-01-01
publisher MDPI AG
record_format Article
series Applied Sciences
spelling doaj.art-8a4b521264d2493483eacc919e1c570e2023-12-03T12:40:54ZengMDPI AGApplied Sciences2076-34172021-01-0111261310.3390/app11020613Absolute Quantitative Volatile Measurement from Fresh Tea Leaves and the Derived Teas Revealed Contributions of Postharvest Synthesis of Endogenous Volatiles for the Aroma Quality of Made TeasMingjie Chen0Li Guo1Huiwen Zhou2Yaling Guo3Yi Zhang4Zhi Lin5Meng Sun6Wei Zeng7Hualing Wu8Henan Key Laboratory of Tea Plant Biology, College of Life Sciences, Xinyang Normal University, Xinyang 464000, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaHenan Key Laboratory of Tea Plant Biology, College of Life Sciences, Xinyang Normal University, Xinyang 464000, ChinaCollege of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaHenan Key Laboratory of Tea Plant Biology, College of Life Sciences, Xinyang Normal University, Xinyang 464000, ChinaTea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 310008, ChinaHenan Key Laboratory of Tea Plant Biology, College of Life Sciences, Xinyang Normal University, Xinyang 464000, ChinaHenan Key Laboratory of Tea Plant Biology, College of Life Sciences, Xinyang Normal University, Xinyang 464000, ChinaTea Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Key Laboratory of Tea Plant Resources Innovation & Utilization, Guangzhou 510640, ChinaCharacteristic aroma is a well-appreciated feature contributing to tea quality. Although extensive studies have been made to investigate aroma biosynthesis and gene expressions during tea making processes, it remains unclear whether the endogenous volatile biosynthesis during postharvest tea processing contributes to the aroma quality of made tea. To critically evaluate this question, we used the same batch of fresh tea leaves and produced three different types of tea with different degrees of fermentation (green tea, oolong tea, and black tea). Total volatiles were extracted by solvent-assisted-flavor evaporation, then quantified by gas chromatography-flame ionization detector combined with response factor correction for quantitative measurement. Compared with fresh tea leaves, the volatile profiles of the made teas were dramatically altered, with significant loss for the majority of endogenous volatiles and simultaneous gain for non-endogenous volatiles. By calculation of odor-activity values, the potential volatiles contributing to the aroma characteristics of each tea type were identified. Our data suggest that postharvest synthesis of endogenous volatiles did not contribute to the aroma quality of made tea.https://www.mdpi.com/2076-3417/11/2/613solvent-assisted-flavor evaporationGC-FIDFID response factorendogenous volatilefresh leavesgreen tea
spellingShingle Mingjie Chen
Li Guo
Huiwen Zhou
Yaling Guo
Yi Zhang
Zhi Lin
Meng Sun
Wei Zeng
Hualing Wu
Absolute Quantitative Volatile Measurement from Fresh Tea Leaves and the Derived Teas Revealed Contributions of Postharvest Synthesis of Endogenous Volatiles for the Aroma Quality of Made Teas
Applied Sciences
solvent-assisted-flavor evaporation
GC-FID
FID response factor
endogenous volatile
fresh leaves
green tea
title Absolute Quantitative Volatile Measurement from Fresh Tea Leaves and the Derived Teas Revealed Contributions of Postharvest Synthesis of Endogenous Volatiles for the Aroma Quality of Made Teas
title_full Absolute Quantitative Volatile Measurement from Fresh Tea Leaves and the Derived Teas Revealed Contributions of Postharvest Synthesis of Endogenous Volatiles for the Aroma Quality of Made Teas
title_fullStr Absolute Quantitative Volatile Measurement from Fresh Tea Leaves and the Derived Teas Revealed Contributions of Postharvest Synthesis of Endogenous Volatiles for the Aroma Quality of Made Teas
title_full_unstemmed Absolute Quantitative Volatile Measurement from Fresh Tea Leaves and the Derived Teas Revealed Contributions of Postharvest Synthesis of Endogenous Volatiles for the Aroma Quality of Made Teas
title_short Absolute Quantitative Volatile Measurement from Fresh Tea Leaves and the Derived Teas Revealed Contributions of Postharvest Synthesis of Endogenous Volatiles for the Aroma Quality of Made Teas
title_sort absolute quantitative volatile measurement from fresh tea leaves and the derived teas revealed contributions of postharvest synthesis of endogenous volatiles for the aroma quality of made teas
topic solvent-assisted-flavor evaporation
GC-FID
FID response factor
endogenous volatile
fresh leaves
green tea
url https://www.mdpi.com/2076-3417/11/2/613
work_keys_str_mv AT mingjiechen absolutequantitativevolatilemeasurementfromfreshtealeavesandthederivedteasrevealedcontributionsofpostharvestsynthesisofendogenousvolatilesforthearomaqualityofmadeteas
AT liguo absolutequantitativevolatilemeasurementfromfreshtealeavesandthederivedteasrevealedcontributionsofpostharvestsynthesisofendogenousvolatilesforthearomaqualityofmadeteas
AT huiwenzhou absolutequantitativevolatilemeasurementfromfreshtealeavesandthederivedteasrevealedcontributionsofpostharvestsynthesisofendogenousvolatilesforthearomaqualityofmadeteas
AT yalingguo absolutequantitativevolatilemeasurementfromfreshtealeavesandthederivedteasrevealedcontributionsofpostharvestsynthesisofendogenousvolatilesforthearomaqualityofmadeteas
AT yizhang absolutequantitativevolatilemeasurementfromfreshtealeavesandthederivedteasrevealedcontributionsofpostharvestsynthesisofendogenousvolatilesforthearomaqualityofmadeteas
AT zhilin absolutequantitativevolatilemeasurementfromfreshtealeavesandthederivedteasrevealedcontributionsofpostharvestsynthesisofendogenousvolatilesforthearomaqualityofmadeteas
AT mengsun absolutequantitativevolatilemeasurementfromfreshtealeavesandthederivedteasrevealedcontributionsofpostharvestsynthesisofendogenousvolatilesforthearomaqualityofmadeteas
AT weizeng absolutequantitativevolatilemeasurementfromfreshtealeavesandthederivedteasrevealedcontributionsofpostharvestsynthesisofendogenousvolatilesforthearomaqualityofmadeteas
AT hualingwu absolutequantitativevolatilemeasurementfromfreshtealeavesandthederivedteasrevealedcontributionsofpostharvestsynthesisofendogenousvolatilesforthearomaqualityofmadeteas