CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte Polarization
M2b monocytes commonly isolated from patients with unhealthy alcohol use (Alc) have been described as cells that make the host susceptible to opportunistic infections. CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> cells are multilineage progenitors of CD19<s...
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MDPI AG
2022-08-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/17/2703 |
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| author | Akira Asai Yusuke Tsuchimoto Hideko Ohama Hiroki Nishikawa Ashok Chopra Kazuhide Higuchi |
| author_facet | Akira Asai Yusuke Tsuchimoto Hideko Ohama Hiroki Nishikawa Ashok Chopra Kazuhide Higuchi |
| author_sort | Akira Asai |
| collection | DOAJ |
| description | M2b monocytes commonly isolated from patients with unhealthy alcohol use (Alc) have been described as cells that make the host susceptible to opportunistic infections. CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> cells are multilineage progenitors of CD19<sup>+</sup> cells, and we show that the effect of these cells from the peripheral blood on M2b monocyte polarization differed between healthy donors and Alc in this study. In healthy donors, these cells consistently differentiated into high-mobility group box-1 (HMGB1)-nonproducing cells (CD19<sup>+</sup> cells) in response to retinoic acid (RA). However, owing to the lack of expression of RA receptor (RAR), these cells from Alc failed to differentiate into CD19<sup>+</sup> cells under the same RA stimulation. Conditioned medium (CM) of these cells from Alc induced the polarization of M2b monocytes, which increases the susceptibility of hosts to opportunistic infections in Alc. When the alcoholic individuals were subjected to 2 weeks of abstinence from alcohol, these cells from Alc recovered their RAR expression and differentiated into CD19<sup>+</sup> cells. Moreover, the CM of these cells from Alc after abstinence lost its ability to induce M2b monocyte polarization. These results indicate that these cells from Alc have different properties from those of healthy donors. In Alc, these cells without RAR stimulate M2b monocyte polarization through the production of HMGB1. |
| first_indexed | 2024-03-10T01:56:58Z |
| format | Article |
| id | doaj.art-8a4fe8e675b549adb397f2dfb477cf12 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T01:56:58Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-8a4fe8e675b549adb397f2dfb477cf122023-11-23T12:55:28ZengMDPI AGCells2073-44092022-08-011117270310.3390/cells11172703CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte PolarizationAkira Asai0Yusuke Tsuchimoto1Hideko Ohama2Hiroki Nishikawa3Ashok Chopra4Kazuhide Higuchi52nd Department of Internal Medicine, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, Japan2nd Department of Internal Medicine, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, Japan2nd Department of Internal Medicine, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, Japan2nd Department of Internal Medicine, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, JapanDepartment of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, TX 77555, USA2nd Department of Internal Medicine, Osaka Medical and Pharmaceutical University, Takatsuki 569-8686, JapanM2b monocytes commonly isolated from patients with unhealthy alcohol use (Alc) have been described as cells that make the host susceptible to opportunistic infections. CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> cells are multilineage progenitors of CD19<sup>+</sup> cells, and we show that the effect of these cells from the peripheral blood on M2b monocyte polarization differed between healthy donors and Alc in this study. In healthy donors, these cells consistently differentiated into high-mobility group box-1 (HMGB1)-nonproducing cells (CD19<sup>+</sup> cells) in response to retinoic acid (RA). However, owing to the lack of expression of RA receptor (RAR), these cells from Alc failed to differentiate into CD19<sup>+</sup> cells under the same RA stimulation. Conditioned medium (CM) of these cells from Alc induced the polarization of M2b monocytes, which increases the susceptibility of hosts to opportunistic infections in Alc. When the alcoholic individuals were subjected to 2 weeks of abstinence from alcohol, these cells from Alc recovered their RAR expression and differentiated into CD19<sup>+</sup> cells. Moreover, the CM of these cells from Alc after abstinence lost its ability to induce M2b monocyte polarization. These results indicate that these cells from Alc have different properties from those of healthy donors. In Alc, these cells without RAR stimulate M2b monocyte polarization through the production of HMGB1.https://www.mdpi.com/2073-4409/11/17/2703patients with unhealthy alcohol use 2CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> cells 3M2b monocytes 4HMGB1 |
| spellingShingle | Akira Asai Yusuke Tsuchimoto Hideko Ohama Hiroki Nishikawa Ashok Chopra Kazuhide Higuchi CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte Polarization Cells patients with unhealthy alcohol use 2 CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> cells 3 M2b monocytes 4 HMGB1 |
| title | CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte Polarization |
| title_full | CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte Polarization |
| title_fullStr | CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte Polarization |
| title_full_unstemmed | CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte Polarization |
| title_short | CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> Cells in Patients with Unhealthy Alcohol Use Stimulate the M2b Monocyte Polarization |
| title_sort | cd34 sup sup cd10 sup sup cd19 sup sup cells in patients with unhealthy alcohol use stimulate the m2b monocyte polarization |
| topic | patients with unhealthy alcohol use 2 CD34<sup>+</sup>CD10<sup>+</sup>CD19<sup>−</sup> cells 3 M2b monocytes 4 HMGB1 |
| url | https://www.mdpi.com/2073-4409/11/17/2703 |
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