<i>Bombyx mori</i> Ecdysone Receptor B1 May Inhibit BmNPV Infection by Triggering Apoptosis
Bombyx mori nucleopolyhedrovirus (BmNPV) is a serious threat to sericulture. Nevertheless, no effective control strategy is currently available. The innate immunity of silkworm is critical in the antiviral process. Exploring its molecular mechanism provides theoretical support for the prevention and...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/6/505 |
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| author | Zhihao Su Chunxiao Zhao Xinming Huang Junli Lv Ziqin Zhao Kaiyi Zheng Xia Sun Sheng Qin Xueyang Wang Byung-Rae Jin Yangchun Wu |
| author_facet | Zhihao Su Chunxiao Zhao Xinming Huang Junli Lv Ziqin Zhao Kaiyi Zheng Xia Sun Sheng Qin Xueyang Wang Byung-Rae Jin Yangchun Wu |
| author_sort | Zhihao Su |
| collection | DOAJ |
| description | Bombyx mori nucleopolyhedrovirus (BmNPV) is a serious threat to sericulture. Nevertheless, no effective control strategy is currently available. The innate immunity of silkworm is critical in the antiviral process. Exploring its molecular mechanism provides theoretical support for the prevention and treatment of BmNPV. Insect hormone receptors play an essential role in regulating host immunity. We found a correlation between <i>Bombyx mori ecdysone receptor B1</i> (<i>BmEcR-B1</i>) and BmNPV infection, whereas the underlying mechanism remains unclear. In this study, the expression patterns and sequence characteristics of <i>BmEcR-B1</i> and its isoform, <i>BmEcR-A</i>, were initially analyzed. <i>BmEcR-B1</i> was found to be more critical than <i>BmEcR-A</i> in silkworm development and responses to BmNPV. Moreover, RNAi and an overexpression in BmN cells showed <i>BmEcR-B1</i> had antiviral effects in the presence of 20-hydroxyecdysone (20E); Otherwise, it had no antiviral activity. Furthermore, <i>BmEcR-B1</i> was required for 20E-induced apoptosis, which significantly suppressed virus infection. Finally, feeding 20E had no significant negative impacts on larval growth and the cocoon shell, suggesting the regulation of this pathway has practical value in controlling BmNPV in sericulture. The findings of this study provide important theoretical support for understanding the mechanism of the silkworm innate immune system in response to BmNPV infection. |
| first_indexed | 2024-03-11T02:19:30Z |
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| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T02:19:30Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-8a5228a181a24cf19767fc5ad1cde3512023-11-18T10:55:45ZengMDPI AGInsects2075-44502023-05-0114650510.3390/insects14060505<i>Bombyx mori</i> Ecdysone Receptor B1 May Inhibit BmNPV Infection by Triggering ApoptosisZhihao Su0Chunxiao Zhao1Xinming Huang2Junli Lv3Ziqin Zhao4Kaiyi Zheng5Xia Sun6Sheng Qin7Xueyang Wang8Byung-Rae Jin9Yangchun Wu10Jiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaCollege of Natural Resources and Life Science, Dong-A University, Busan 49315, Republic of KoreaJiangsu Key Laboratory of Sericultural Biology and Biotechnology, School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 212100, ChinaBombyx mori nucleopolyhedrovirus (BmNPV) is a serious threat to sericulture. Nevertheless, no effective control strategy is currently available. The innate immunity of silkworm is critical in the antiviral process. Exploring its molecular mechanism provides theoretical support for the prevention and treatment of BmNPV. Insect hormone receptors play an essential role in regulating host immunity. We found a correlation between <i>Bombyx mori ecdysone receptor B1</i> (<i>BmEcR-B1</i>) and BmNPV infection, whereas the underlying mechanism remains unclear. In this study, the expression patterns and sequence characteristics of <i>BmEcR-B1</i> and its isoform, <i>BmEcR-A</i>, were initially analyzed. <i>BmEcR-B1</i> was found to be more critical than <i>BmEcR-A</i> in silkworm development and responses to BmNPV. Moreover, RNAi and an overexpression in BmN cells showed <i>BmEcR-B1</i> had antiviral effects in the presence of 20-hydroxyecdysone (20E); Otherwise, it had no antiviral activity. Furthermore, <i>BmEcR-B1</i> was required for 20E-induced apoptosis, which significantly suppressed virus infection. Finally, feeding 20E had no significant negative impacts on larval growth and the cocoon shell, suggesting the regulation of this pathway has practical value in controlling BmNPV in sericulture. The findings of this study provide important theoretical support for understanding the mechanism of the silkworm innate immune system in response to BmNPV infection.https://www.mdpi.com/2075-4450/14/6/505<i>Bombyx mori</i><i>BmEcR-B1</i>BmNPV20Eapoptosis |
| spellingShingle | Zhihao Su Chunxiao Zhao Xinming Huang Junli Lv Ziqin Zhao Kaiyi Zheng Xia Sun Sheng Qin Xueyang Wang Byung-Rae Jin Yangchun Wu <i>Bombyx mori</i> Ecdysone Receptor B1 May Inhibit BmNPV Infection by Triggering Apoptosis Insects <i>Bombyx mori</i> <i>BmEcR-B1</i> BmNPV 20E apoptosis |
| title | <i>Bombyx mori</i> Ecdysone Receptor B1 May Inhibit BmNPV Infection by Triggering Apoptosis |
| title_full | <i>Bombyx mori</i> Ecdysone Receptor B1 May Inhibit BmNPV Infection by Triggering Apoptosis |
| title_fullStr | <i>Bombyx mori</i> Ecdysone Receptor B1 May Inhibit BmNPV Infection by Triggering Apoptosis |
| title_full_unstemmed | <i>Bombyx mori</i> Ecdysone Receptor B1 May Inhibit BmNPV Infection by Triggering Apoptosis |
| title_short | <i>Bombyx mori</i> Ecdysone Receptor B1 May Inhibit BmNPV Infection by Triggering Apoptosis |
| title_sort | i bombyx mori i ecdysone receptor b1 may inhibit bmnpv infection by triggering apoptosis |
| topic | <i>Bombyx mori</i> <i>BmEcR-B1</i> BmNPV 20E apoptosis |
| url | https://www.mdpi.com/2075-4450/14/6/505 |
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