Longitudinal Follow-Up of the Immunity to SARS-CoV-2 in Health Care Workers in Argentina: Persistence of Humoral Response and Neutralizing Capacity after Sputnik V Vaccination
ABSTRACT SARS-CoV-2 vaccine protection has encountered waning of immune response and breakthrough infections. The hybrid immune response generated by the combination of vaccination and infection was shown to offer higher and broader protection. Here, we present a seroprevalence study of anti-SARS-Co...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
American Society for Microbiology
2023-06-01
|
| Series: | mSphere |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/msphere.00662-22 |
| _version_ | 1827919024252518400 |
|---|---|
| author | Eliana F. Castro Julián Acosta Lucía Moriena Mayra Rios Medrano Malena Tejerina Cibello Eduardo Codino Miguel Ángel Taborda Diego E. Álvarez Ana Laura Cavatorta |
| author_facet | Eliana F. Castro Julián Acosta Lucía Moriena Mayra Rios Medrano Malena Tejerina Cibello Eduardo Codino Miguel Ángel Taborda Diego E. Álvarez Ana Laura Cavatorta |
| author_sort | Eliana F. Castro |
| collection | DOAJ |
| description | ABSTRACT SARS-CoV-2 vaccine protection has encountered waning of immune response and breakthrough infections. The hybrid immune response generated by the combination of vaccination and infection was shown to offer higher and broader protection. Here, we present a seroprevalence study of anti-SARS-CoV-2 spike/RBD IgG in 1,121 health care workers immunized with Sputnik V and a follow-up of humoral response at 2 and 24 weeks postvaccination (wpv), including neutralizing antibody response (NAT) against ancestral, Gamma, and Delta variants. The first seroprevalence study showed that among 122 individuals with one dose, 90.2% were seropositive versus 99.7% seropositivity among volunteers with the complete two-dose regimen. At 24 wpv, 98.7% of the volunteers remained seropositive, although antibody levels decreased. IgG levels and NAT were higher in individuals that had acquired COVID-19 previous to vaccination than in naive individuals at 2 and 24 wpv. Antibody levels dropped over time in both groups. In contrast, IgG levels and NAT increased after vaccine breakthrough infection. At 2 wpv, 35/40 naive individuals had detectable NAT against SARS-CoV-2 Gamma and 6/40 against Delta. In turn, 8/9 previously infected individuals developed a neutralizing response against SARS-CoV-2 Gamma and 4/9 against Delta variants. NAT against variants followed a trajectory similar to NAT against ancestral SARS-CoV-2, and breakthrough infection led to an increase in NAT and complete seroconversion against variants. In conclusion, Sputnik V-induced humoral response persisted at 6 months postvaccination, and hybrid immunity induced higher levels of anti-S/RBD antibodies and NAT in previously exposed individuals, boosted the response after vaccination, and conferred wider breadth of protection. IMPORTANCE Since December 2020, Argentina has begun a mass vaccination program. The first vaccine available in our country was Sputnik V, which has been approved for use in 71 countries with a total population of 4 billion people. Despite all the available information, there are fewer published studies on the response induced by Sputnik V vaccination compared to that of other vaccines. Although the global political context has paralyzed the verification by the WHO of the efficacy of this vaccine, our work aims to add new clear and necessary evidence to Sputnik V performance. Our results contribute to general knowledge of the humoral immune response developed by vaccines based on viral vector technology, highlighting the higher immune protection conferred by hybrid immunity and reinforcing the importance of completing vaccination schedules and booster doses to maintain adequate antibody levels. |
| first_indexed | 2024-03-13T03:51:54Z |
| format | Article |
| id | doaj.art-8a539e19bf004a3bbea985e2b005c414 |
| institution | Directory Open Access Journal |
| issn | 2379-5042 |
| language | English |
| last_indexed | 2024-03-13T03:51:54Z |
| publishDate | 2023-06-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | mSphere |
| spelling | doaj.art-8a539e19bf004a3bbea985e2b005c4142023-06-22T13:00:23ZengAmerican Society for MicrobiologymSphere2379-50422023-06-018310.1128/msphere.00662-22Longitudinal Follow-Up of the Immunity to SARS-CoV-2 in Health Care Workers in Argentina: Persistence of Humoral Response and Neutralizing Capacity after Sputnik V VaccinationEliana F. Castro0Julián Acosta1Lucía Moriena2Mayra Rios Medrano3Malena Tejerina Cibello4Eduardo Codino5Miguel Ángel Taborda6Diego E. Álvarez7Ana Laura Cavatorta8Instituto de Investigaciones Biotecnológicas (IIBIO), Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinaInstituto de Biología Molecular y Celular de Rosario-CONICET, Santa Fe, ArgentinaCentro de Tecnología en Salud Pública (CTSP), Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Santa Fe, ArgentinaInstituto de Investigaciones Biotecnológicas (IIBIO), Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinaEscuela de Bio y Nanotecnologías (EByN), Universidad Nacional de San Martín, Buenos Aires, ArgentinaFacultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Santa Fe, ArgentinaFacultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Santa Fe, ArgentinaInstituto de Investigaciones Biotecnológicas (IIBIO), Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, ArgentinaInstituto de Biología Molecular y Celular de Rosario-CONICET, Santa Fe, ArgentinaABSTRACT SARS-CoV-2 vaccine protection has encountered waning of immune response and breakthrough infections. The hybrid immune response generated by the combination of vaccination and infection was shown to offer higher and broader protection. Here, we present a seroprevalence study of anti-SARS-CoV-2 spike/RBD IgG in 1,121 health care workers immunized with Sputnik V and a follow-up of humoral response at 2 and 24 weeks postvaccination (wpv), including neutralizing antibody response (NAT) against ancestral, Gamma, and Delta variants. The first seroprevalence study showed that among 122 individuals with one dose, 90.2% were seropositive versus 99.7% seropositivity among volunteers with the complete two-dose regimen. At 24 wpv, 98.7% of the volunteers remained seropositive, although antibody levels decreased. IgG levels and NAT were higher in individuals that had acquired COVID-19 previous to vaccination than in naive individuals at 2 and 24 wpv. Antibody levels dropped over time in both groups. In contrast, IgG levels and NAT increased after vaccine breakthrough infection. At 2 wpv, 35/40 naive individuals had detectable NAT against SARS-CoV-2 Gamma and 6/40 against Delta. In turn, 8/9 previously infected individuals developed a neutralizing response against SARS-CoV-2 Gamma and 4/9 against Delta variants. NAT against variants followed a trajectory similar to NAT against ancestral SARS-CoV-2, and breakthrough infection led to an increase in NAT and complete seroconversion against variants. In conclusion, Sputnik V-induced humoral response persisted at 6 months postvaccination, and hybrid immunity induced higher levels of anti-S/RBD antibodies and NAT in previously exposed individuals, boosted the response after vaccination, and conferred wider breadth of protection. IMPORTANCE Since December 2020, Argentina has begun a mass vaccination program. The first vaccine available in our country was Sputnik V, which has been approved for use in 71 countries with a total population of 4 billion people. Despite all the available information, there are fewer published studies on the response induced by Sputnik V vaccination compared to that of other vaccines. Although the global political context has paralyzed the verification by the WHO of the efficacy of this vaccine, our work aims to add new clear and necessary evidence to Sputnik V performance. Our results contribute to general knowledge of the humoral immune response developed by vaccines based on viral vector technology, highlighting the higher immune protection conferred by hybrid immunity and reinforcing the importance of completing vaccination schedules and booster doses to maintain adequate antibody levels.https://journals.asm.org/doi/10.1128/msphere.00662-22SARS-CoV-2Sputnik Vhumoral immune responsehybrid immunityvaccination |
| spellingShingle | Eliana F. Castro Julián Acosta Lucía Moriena Mayra Rios Medrano Malena Tejerina Cibello Eduardo Codino Miguel Ángel Taborda Diego E. Álvarez Ana Laura Cavatorta Longitudinal Follow-Up of the Immunity to SARS-CoV-2 in Health Care Workers in Argentina: Persistence of Humoral Response and Neutralizing Capacity after Sputnik V Vaccination mSphere SARS-CoV-2 Sputnik V humoral immune response hybrid immunity vaccination |
| title | Longitudinal Follow-Up of the Immunity to SARS-CoV-2 in Health Care Workers in Argentina: Persistence of Humoral Response and Neutralizing Capacity after Sputnik V Vaccination |
| title_full | Longitudinal Follow-Up of the Immunity to SARS-CoV-2 in Health Care Workers in Argentina: Persistence of Humoral Response and Neutralizing Capacity after Sputnik V Vaccination |
| title_fullStr | Longitudinal Follow-Up of the Immunity to SARS-CoV-2 in Health Care Workers in Argentina: Persistence of Humoral Response and Neutralizing Capacity after Sputnik V Vaccination |
| title_full_unstemmed | Longitudinal Follow-Up of the Immunity to SARS-CoV-2 in Health Care Workers in Argentina: Persistence of Humoral Response and Neutralizing Capacity after Sputnik V Vaccination |
| title_short | Longitudinal Follow-Up of the Immunity to SARS-CoV-2 in Health Care Workers in Argentina: Persistence of Humoral Response and Neutralizing Capacity after Sputnik V Vaccination |
| title_sort | longitudinal follow up of the immunity to sars cov 2 in health care workers in argentina persistence of humoral response and neutralizing capacity after sputnik v vaccination |
| topic | SARS-CoV-2 Sputnik V humoral immune response hybrid immunity vaccination |
| url | https://journals.asm.org/doi/10.1128/msphere.00662-22 |
| work_keys_str_mv | AT elianafcastro longitudinalfollowupoftheimmunitytosarscov2inhealthcareworkersinargentinapersistenceofhumoralresponseandneutralizingcapacityaftersputnikvvaccination AT julianacosta longitudinalfollowupoftheimmunitytosarscov2inhealthcareworkersinargentinapersistenceofhumoralresponseandneutralizingcapacityaftersputnikvvaccination AT luciamoriena longitudinalfollowupoftheimmunitytosarscov2inhealthcareworkersinargentinapersistenceofhumoralresponseandneutralizingcapacityaftersputnikvvaccination AT mayrariosmedrano longitudinalfollowupoftheimmunitytosarscov2inhealthcareworkersinargentinapersistenceofhumoralresponseandneutralizingcapacityaftersputnikvvaccination AT malenatejerinacibello longitudinalfollowupoftheimmunitytosarscov2inhealthcareworkersinargentinapersistenceofhumoralresponseandneutralizingcapacityaftersputnikvvaccination AT eduardocodino longitudinalfollowupoftheimmunitytosarscov2inhealthcareworkersinargentinapersistenceofhumoralresponseandneutralizingcapacityaftersputnikvvaccination AT miguelangeltaborda longitudinalfollowupoftheimmunitytosarscov2inhealthcareworkersinargentinapersistenceofhumoralresponseandneutralizingcapacityaftersputnikvvaccination AT diegoealvarez longitudinalfollowupoftheimmunitytosarscov2inhealthcareworkersinargentinapersistenceofhumoralresponseandneutralizingcapacityaftersputnikvvaccination AT analauracavatorta longitudinalfollowupoftheimmunitytosarscov2inhealthcareworkersinargentinapersistenceofhumoralresponseandneutralizingcapacityaftersputnikvvaccination |