Marsdenia tenacissima enhances immune response of tumor infiltrating T lymphocytes to colorectal cancer
IntroductionTumor-infiltrating T lymphocytes in the tumor microenvironment are critical factors influencing the prognosis and chemotherapy outcomes. As a Chinese herbal medicine, Marsdenia tenacissima extract (MTE) has been widely used to treat cancer in China. Its immunoregulatory effects on tumor-...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-08-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1238694/full |
| _version_ | 1797742253585727488 |
|---|---|
| author | Ben Yi Ben Yi Shuai Zhang Suying Yan Suying Yan Yanfei Liu Yanfei Liu Zhiqiang Feng Zhiqiang Feng Tianhao Chu Tianhao Chu Jun Liu Wei Wang Jun Xue Chunze Zhang Yijia Wang |
| author_facet | Ben Yi Ben Yi Shuai Zhang Suying Yan Suying Yan Yanfei Liu Yanfei Liu Zhiqiang Feng Zhiqiang Feng Tianhao Chu Tianhao Chu Jun Liu Wei Wang Jun Xue Chunze Zhang Yijia Wang |
| author_sort | Ben Yi |
| collection | DOAJ |
| description | IntroductionTumor-infiltrating T lymphocytes in the tumor microenvironment are critical factors influencing the prognosis and chemotherapy outcomes. As a Chinese herbal medicine, Marsdenia tenacissima extract (MTE) has been widely used to treat cancer in China. Its immunoregulatory effects on tumor-associated macrophages is well known, but whether it regulates tumor-infiltrating T-cell functions remains unclear.MethodWe collected 17 tumor samples from MTE-administered colorectal cancer patients, 13 of which showed upregulation of CD3+/CD8+ tumor-infiltrating T cells. Further in vitro and in vivo experiments were performed to investigate the regulatory effects of MTE on tumor-infiltrating T cells and immune escape of tumors.ResultsUnder single and co-culture conditions, MTE inhibited TGF-β1 and PD-L1 expression in the colorectal cancer (CRC) cell lines HCT116 and LoVo. In Jurkat cells, MTE inhibited FOXP3 and IL-10 expression, increased IL-2 expression, but had no effect on PD-1 expression. These findings were confirmed in vitro using subcutaneous and colitis-associated CRC mouse models. MTE also increased the density of CD3+/CD8+ tumor-infiltrating T cells and exhibited considerable tumor-suppressive effects in these two tumor mouse models.ConclusionsOur findings suggested that MTE inhibits the immune escape of cancer cells, a precipitating factor increasing the immune response of T lymphocytes. |
| first_indexed | 2024-03-12T14:38:19Z |
| format | Article |
| id | doaj.art-8a57bfbe48244c9fb233956cabc6c196 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-12T14:38:19Z |
| publishDate | 2023-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-8a57bfbe48244c9fb233956cabc6c1962023-08-17T00:44:20ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-08-011410.3389/fimmu.2023.12386941238694Marsdenia tenacissima enhances immune response of tumor infiltrating T lymphocytes to colorectal cancerBen Yi0Ben Yi1Shuai Zhang2Suying Yan3Suying Yan4Yanfei Liu5Yanfei Liu6Zhiqiang Feng7Zhiqiang Feng8Tianhao Chu9Tianhao Chu10Jun Liu11Wei Wang12Jun Xue13Chunze Zhang14Yijia Wang15School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaDepartment of Colorectal Surgery, Tianjin Union Medical Center, Tianjin, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaDepartment of Colorectal Surgery, Tianjin Union Medical Center, Tianjin, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaDepartment of Colorectal Surgery, Tianjin Union Medical Center, Tianjin, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaDepartment of Colorectal Surgery, Tianjin Union Medical Center, Tianjin, ChinaSchool of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, ChinaDepartment of Colorectal Surgery, Tianjin Union Medical Center, Tianjin, ChinaDepartment of Radiology, The Fourth Central Hospital Affiliated to Nankai University, Tianjin, ChinaTEDA Institute of Biological Sciences and Biotechnology, Nankai University, Tianjin, ChinaDepartment of General Surgery, The First Affiliated Hospital of Hebei North University, Zhangjiakou, ChinaDepartment of Colorectal Surgery, Tianjin Union Medical Center, Tianjin, ChinaLaboratory of Oncologic Molecular Medicine, Tianjin Union Medical Center, Tianjin, ChinaIntroductionTumor-infiltrating T lymphocytes in the tumor microenvironment are critical factors influencing the prognosis and chemotherapy outcomes. As a Chinese herbal medicine, Marsdenia tenacissima extract (MTE) has been widely used to treat cancer in China. Its immunoregulatory effects on tumor-associated macrophages is well known, but whether it regulates tumor-infiltrating T-cell functions remains unclear.MethodWe collected 17 tumor samples from MTE-administered colorectal cancer patients, 13 of which showed upregulation of CD3+/CD8+ tumor-infiltrating T cells. Further in vitro and in vivo experiments were performed to investigate the regulatory effects of MTE on tumor-infiltrating T cells and immune escape of tumors.ResultsUnder single and co-culture conditions, MTE inhibited TGF-β1 and PD-L1 expression in the colorectal cancer (CRC) cell lines HCT116 and LoVo. In Jurkat cells, MTE inhibited FOXP3 and IL-10 expression, increased IL-2 expression, but had no effect on PD-1 expression. These findings were confirmed in vitro using subcutaneous and colitis-associated CRC mouse models. MTE also increased the density of CD3+/CD8+ tumor-infiltrating T cells and exhibited considerable tumor-suppressive effects in these two tumor mouse models.ConclusionsOur findings suggested that MTE inhibits the immune escape of cancer cells, a precipitating factor increasing the immune response of T lymphocytes.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1238694/fullmarsdenia tenacissima extractiontumor microenvironmenttumor infiltrating T cellsCD8colorectal cancer |
| spellingShingle | Ben Yi Ben Yi Shuai Zhang Suying Yan Suying Yan Yanfei Liu Yanfei Liu Zhiqiang Feng Zhiqiang Feng Tianhao Chu Tianhao Chu Jun Liu Wei Wang Jun Xue Chunze Zhang Yijia Wang Marsdenia tenacissima enhances immune response of tumor infiltrating T lymphocytes to colorectal cancer Frontiers in Immunology marsdenia tenacissima extraction tumor microenvironment tumor infiltrating T cells CD8 colorectal cancer |
| title | Marsdenia tenacissima enhances immune response of tumor infiltrating T lymphocytes to colorectal cancer |
| title_full | Marsdenia tenacissima enhances immune response of tumor infiltrating T lymphocytes to colorectal cancer |
| title_fullStr | Marsdenia tenacissima enhances immune response of tumor infiltrating T lymphocytes to colorectal cancer |
| title_full_unstemmed | Marsdenia tenacissima enhances immune response of tumor infiltrating T lymphocytes to colorectal cancer |
| title_short | Marsdenia tenacissima enhances immune response of tumor infiltrating T lymphocytes to colorectal cancer |
| title_sort | marsdenia tenacissima enhances immune response of tumor infiltrating t lymphocytes to colorectal cancer |
| topic | marsdenia tenacissima extraction tumor microenvironment tumor infiltrating T cells CD8 colorectal cancer |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1238694/full |
| work_keys_str_mv | AT benyi marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT benyi marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT shuaizhang marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT suyingyan marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT suyingyan marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT yanfeiliu marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT yanfeiliu marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT zhiqiangfeng marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT zhiqiangfeng marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT tianhaochu marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT tianhaochu marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT junliu marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT weiwang marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT junxue marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT chunzezhang marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer AT yijiawang marsdeniatenacissimaenhancesimmuneresponseoftumorinfiltratingtlymphocytestocolorectalcancer |