Ubiquitin-specific protease 53 promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells
Abstract The ubiquitin protease pathway plays important role in human bone marrow-derived mesenchymal stem cell (hBMSC) differentiation, including osteogenesis. However, the function of deubiquitinating enzymes in osteogenic differentiation of hBMSCs remains poorly understood. In this study, we aime...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2021-03-01
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| Series: | Cell Death and Disease |
| Online Access: | https://doi.org/10.1038/s41419-021-03517-x |
| _version_ | 1819163864012423168 |
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| author | Dawoon Baek Kwang Hwan Park Kyoung-Mi Lee Sujin Jung Soyeong Joung Jihyun Kim Jin Woo Lee |
| author_facet | Dawoon Baek Kwang Hwan Park Kyoung-Mi Lee Sujin Jung Soyeong Joung Jihyun Kim Jin Woo Lee |
| author_sort | Dawoon Baek |
| collection | DOAJ |
| description | Abstract The ubiquitin protease pathway plays important role in human bone marrow-derived mesenchymal stem cell (hBMSC) differentiation, including osteogenesis. However, the function of deubiquitinating enzymes in osteogenic differentiation of hBMSCs remains poorly understood. In this study, we aimed to investigate the role of ubiquitin-specific protease 53 (USP53) in the osteogenic differentiation of hBMSCs. Based on re-analysis of the Gene Expression Omnibus database, USP53 was selected as a positive regulator of osteogenic differentiation in hBMSCs. Overexpression of USP53 by lentivirus enhanced osteogenesis in hBMSCs, whereas knockdown of USP53 by lentivirus inhibited osteogenesis in hBMSCs. In addition, USP53 overexpression increased the level of active β-catenin and enhanced the osteogenic differentiation of hBMSCs. This effect was reversed by the Wnt/β-catenin inhibitor DKK1. Mass spectrometry showed that USP53 interacted with F-box only protein 31 (FBXO31) to promote proteasomal degradation of β-catenin. Inhibition of the osteogenic differentiation of hBMSCs by FBXO31 was partially rescued by USP53 overexpression. Animal studies showed that hBMSCs with USP53 overexpression significantly promoted bone regeneration in mice with calvarial defects. These results suggested that USP53 may be a target for gene therapy for bone regeneration. |
| first_indexed | 2024-12-22T17:50:54Z |
| format | Article |
| id | doaj.art-8a58d6f2809e470d9d9687d51a9328c3 |
| institution | Directory Open Access Journal |
| issn | 2041-4889 |
| language | English |
| last_indexed | 2024-12-22T17:50:54Z |
| publishDate | 2021-03-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death and Disease |
| spelling | doaj.art-8a58d6f2809e470d9d9687d51a9328c32022-12-21T18:18:10ZengNature Publishing GroupCell Death and Disease2041-48892021-03-0112311510.1038/s41419-021-03517-xUbiquitin-specific protease 53 promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cellsDawoon Baek0Kwang Hwan Park1Kyoung-Mi Lee2Sujin Jung3Soyeong Joung4Jihyun Kim5Jin Woo Lee6Department of Orthopaedic Surgery, Yonsei University College of MedicineDepartment of Orthopaedic Surgery, Yonsei University College of MedicineDepartment of Orthopaedic Surgery, Yonsei University College of MedicineDepartment of Orthopaedic Surgery, Yonsei University College of MedicineDepartment of Orthopaedic Surgery, Yonsei University College of MedicineDepartment of Orthopaedic Surgery, Yonsei University College of MedicineDepartment of Orthopaedic Surgery, Yonsei University College of MedicineAbstract The ubiquitin protease pathway plays important role in human bone marrow-derived mesenchymal stem cell (hBMSC) differentiation, including osteogenesis. However, the function of deubiquitinating enzymes in osteogenic differentiation of hBMSCs remains poorly understood. In this study, we aimed to investigate the role of ubiquitin-specific protease 53 (USP53) in the osteogenic differentiation of hBMSCs. Based on re-analysis of the Gene Expression Omnibus database, USP53 was selected as a positive regulator of osteogenic differentiation in hBMSCs. Overexpression of USP53 by lentivirus enhanced osteogenesis in hBMSCs, whereas knockdown of USP53 by lentivirus inhibited osteogenesis in hBMSCs. In addition, USP53 overexpression increased the level of active β-catenin and enhanced the osteogenic differentiation of hBMSCs. This effect was reversed by the Wnt/β-catenin inhibitor DKK1. Mass spectrometry showed that USP53 interacted with F-box only protein 31 (FBXO31) to promote proteasomal degradation of β-catenin. Inhibition of the osteogenic differentiation of hBMSCs by FBXO31 was partially rescued by USP53 overexpression. Animal studies showed that hBMSCs with USP53 overexpression significantly promoted bone regeneration in mice with calvarial defects. These results suggested that USP53 may be a target for gene therapy for bone regeneration.https://doi.org/10.1038/s41419-021-03517-x |
| spellingShingle | Dawoon Baek Kwang Hwan Park Kyoung-Mi Lee Sujin Jung Soyeong Joung Jihyun Kim Jin Woo Lee Ubiquitin-specific protease 53 promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells Cell Death and Disease |
| title | Ubiquitin-specific protease 53 promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells |
| title_full | Ubiquitin-specific protease 53 promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells |
| title_fullStr | Ubiquitin-specific protease 53 promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells |
| title_full_unstemmed | Ubiquitin-specific protease 53 promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells |
| title_short | Ubiquitin-specific protease 53 promotes osteogenic differentiation of human bone marrow-derived mesenchymal stem cells |
| title_sort | ubiquitin specific protease 53 promotes osteogenic differentiation of human bone marrow derived mesenchymal stem cells |
| url | https://doi.org/10.1038/s41419-021-03517-x |
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