Enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routes
Silkworm pupa is a nourishing food with high nutritional value, but its consumption has been greatly limited given its allergenicity. Enzyme hydrolytic technique is recognized as an effective method to reduce the allergenicity of protein. In this study, we aimed to investigate the effect of enzymoly...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tsinghua University Press
2023-05-01
|
| Series: | Food Science and Human Wellness |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022001987 |
| _version_ | 1797705859284860928 |
|---|---|
| author | Yan Dai Meijia Huang Yujuan Xu Lixia Mu Jingyan Gao Hongbing Chen Zhihua Wu Anshu Yang Yong Wu Xin Li |
| author_facet | Yan Dai Meijia Huang Yujuan Xu Lixia Mu Jingyan Gao Hongbing Chen Zhihua Wu Anshu Yang Yong Wu Xin Li |
| author_sort | Yan Dai |
| collection | DOAJ |
| description | Silkworm pupa is a nourishing food with high nutritional value, but its consumption has been greatly limited given its allergenicity. Enzyme hydrolytic technique is recognized as an effective method to reduce the allergenicity of protein. In this study, we aimed to investigate the effect of enzymolysis on the allergenicity of silkworm pupa. Crude silkworm pupa protein was extracted through alkali extraction and acid precipitation, which included 5 proteins with the molecular weights ranging from 34 kDa to 76 kDa, and silkworm pupa were then hydrolyzed by alkaline protease. The allergenicity of silkworm pupa protein and its enzymatic hydrolysates was evaluated by establishing BALB/c mice model, and the mice were immunized via intragastric gavage and intraperitoneal injection, respectively. The results indicated that the intraperitoneal injection immunization route induced more by detecting with antibodies, histamine and Th2-related cytokines. Moreover, mice treated with silkworm pupa protein peptide displayed no obvious allergic symptoms, indicating that enzyme hydrolytic technique could significantly reduce the allergenicity of silkworm pupa. |
| first_indexed | 2024-03-12T05:43:37Z |
| format | Article |
| id | doaj.art-8a59b38697f742579ff39feea702e474 |
| institution | Directory Open Access Journal |
| issn | 2213-4530 |
| language | English |
| last_indexed | 2024-03-12T05:43:37Z |
| publishDate | 2023-05-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj.art-8a59b38697f742579ff39feea702e4742023-09-03T05:51:14ZengTsinghua University PressFood Science and Human Wellness2213-45302023-05-01123774782Enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routesYan Dai0Meijia Huang1Yujuan Xu2Lixia Mu3Jingyan Gao4Hongbing Chen5Zhihua Wu6Anshu Yang7Yong Wu8Xin Li9State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; School of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; School of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaSericultural and Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSericultural and Agri-Food Research Institute Guangdong Academy of Agricultural Sciences, Key Laboratory of Functional Foods, Ministry of Agriculture and Rural Affairs, Guangdong Key Laboratory of Agricultural Products Processing, Guangzhou 510610, ChinaSchool of Food Science and Technology, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, ChinaState Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China; School of Food Science and Technology, Nanchang University, Nanchang 330047, China; Corresponding author at: State Key Laboratory of Food Science and Technology, Nanchang University, 235 Nanjing Donglu, Nanchang 330047, China.Silkworm pupa is a nourishing food with high nutritional value, but its consumption has been greatly limited given its allergenicity. Enzyme hydrolytic technique is recognized as an effective method to reduce the allergenicity of protein. In this study, we aimed to investigate the effect of enzymolysis on the allergenicity of silkworm pupa. Crude silkworm pupa protein was extracted through alkali extraction and acid precipitation, which included 5 proteins with the molecular weights ranging from 34 kDa to 76 kDa, and silkworm pupa were then hydrolyzed by alkaline protease. The allergenicity of silkworm pupa protein and its enzymatic hydrolysates was evaluated by establishing BALB/c mice model, and the mice were immunized via intragastric gavage and intraperitoneal injection, respectively. The results indicated that the intraperitoneal injection immunization route induced more by detecting with antibodies, histamine and Th2-related cytokines. Moreover, mice treated with silkworm pupa protein peptide displayed no obvious allergic symptoms, indicating that enzyme hydrolytic technique could significantly reduce the allergenicity of silkworm pupa.http://www.sciencedirect.com/science/article/pii/S2213453022001987Silkworm pupaSilkworm pupa peptidesEnzymatic hydrolysisAllergenicityImmunizationAnimal model |
| spellingShingle | Yan Dai Meijia Huang Yujuan Xu Lixia Mu Jingyan Gao Hongbing Chen Zhihua Wu Anshu Yang Yong Wu Xin Li Enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routes Food Science and Human Wellness Silkworm pupa Silkworm pupa peptides Enzymatic hydrolysis Allergenicity Immunization Animal model |
| title | Enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routes |
| title_full | Enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routes |
| title_fullStr | Enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routes |
| title_full_unstemmed | Enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routes |
| title_short | Enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routes |
| title_sort | enzymatic hydrolysis of silkworm pupa and its allergenicity evaluation by animal model with different immunization routes |
| topic | Silkworm pupa Silkworm pupa peptides Enzymatic hydrolysis Allergenicity Immunization Animal model |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022001987 |
| work_keys_str_mv | AT yandai enzymatichydrolysisofsilkwormpupaanditsallergenicityevaluationbyanimalmodelwithdifferentimmunizationroutes AT meijiahuang enzymatichydrolysisofsilkwormpupaanditsallergenicityevaluationbyanimalmodelwithdifferentimmunizationroutes AT yujuanxu enzymatichydrolysisofsilkwormpupaanditsallergenicityevaluationbyanimalmodelwithdifferentimmunizationroutes AT lixiamu enzymatichydrolysisofsilkwormpupaanditsallergenicityevaluationbyanimalmodelwithdifferentimmunizationroutes AT jingyangao enzymatichydrolysisofsilkwormpupaanditsallergenicityevaluationbyanimalmodelwithdifferentimmunizationroutes AT hongbingchen enzymatichydrolysisofsilkwormpupaanditsallergenicityevaluationbyanimalmodelwithdifferentimmunizationroutes AT zhihuawu enzymatichydrolysisofsilkwormpupaanditsallergenicityevaluationbyanimalmodelwithdifferentimmunizationroutes AT anshuyang enzymatichydrolysisofsilkwormpupaanditsallergenicityevaluationbyanimalmodelwithdifferentimmunizationroutes AT yongwu enzymatichydrolysisofsilkwormpupaanditsallergenicityevaluationbyanimalmodelwithdifferentimmunizationroutes AT xinli enzymatichydrolysisofsilkwormpupaanditsallergenicityevaluationbyanimalmodelwithdifferentimmunizationroutes |