Antioxidant potential of garlic oil supplementation prevents cyclophosphamide-induced oxidative testicular damage and endocrine depletion in rats
Cyclophosphamide is an alkylating anticancer agent with strong efficacy; however, its clinical use is constrained because of its off-target multiple organ toxicity, and one of them is testicular injury. We assayed to explore whether garlic oil (GO) could prevent cyclophosphamide (CYP)-induced testic...
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| Format: | Article |
| Language: | English |
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Elsevier
2019-12-01
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| Series: | Journal of Nutrition & Intermediary Metabolism |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352385920300013 |
| _version_ | 1811204677094604800 |
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| author | Chima A. Ekeleme-Egedigwe Ademola C. Famurewa Ebuka E. David Chinedum O. Eleazu Uchenna O. Egedigwe |
| author_facet | Chima A. Ekeleme-Egedigwe Ademola C. Famurewa Ebuka E. David Chinedum O. Eleazu Uchenna O. Egedigwe |
| author_sort | Chima A. Ekeleme-Egedigwe |
| collection | DOAJ |
| description | Cyclophosphamide is an alkylating anticancer agent with strong efficacy; however, its clinical use is constrained because of its off-target multiple organ toxicity, and one of them is testicular injury. We assayed to explore whether garlic oil (GO) could prevent cyclophosphamide (CYP)-induced testicular oxidative stress and hormonal deficit in male rats. Rats were pretreated with GO for 21 days before a single injection of CP (50 mg/kg, ip). The total phenol and flavonoids of GO were estimated as well as its antioxidant capacity using DPPH and FRAP assays. CYP induced prominent depression in testicular activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and reduced glutathione (GSH) level, whereas levels of malondialdehyde (MDA) markedly increased and confirmed by histopathological alterations. Serum levels of testosterone, FSH and LH were considerably reduced. Interestingly, the GO supplementation attenuated the biochemical changes in the testis, enhanced the hormone levels and alleviated the histological injury. The IC50 of GO in DPPH assay was comparable to that of standard. GO is capable of protecting the testis from CYP toxicity via its antioxidant property. The findings suggest GO beneficial effects in male cancer patients undergoing CYP chemotherapy. Keywords: Garlic oil, Cyclophosphamide, Testosterone, Supplementation, Antioxidant |
| first_indexed | 2024-04-12T03:17:44Z |
| format | Article |
| id | doaj.art-8a5f4c613ec24e5c9f214ff5cd89d302 |
| institution | Directory Open Access Journal |
| issn | 2352-3859 |
| language | English |
| last_indexed | 2024-04-12T03:17:44Z |
| publishDate | 2019-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Nutrition & Intermediary Metabolism |
| spelling | doaj.art-8a5f4c613ec24e5c9f214ff5cd89d3022022-12-22T03:50:02ZengElsevierJournal of Nutrition & Intermediary Metabolism2352-38592019-12-0118Antioxidant potential of garlic oil supplementation prevents cyclophosphamide-induced oxidative testicular damage and endocrine depletion in ratsChima A. Ekeleme-Egedigwe0Ademola C. Famurewa1Ebuka E. David2Chinedum O. Eleazu3Uchenna O. Egedigwe4Department of Chemistry/Biochemistry and Molecular Biology, Faculty of Science, Alex-Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, NigeriaDepartment of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Alex Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria; Biochemistry Division, Amala Cancer Research Centre, Amala Institute of Medical Sciences, Amala Nagar P O, Thrissur, 680555, Kerala, India; Corresponding author. Department of Medical Biochemistry, Faculty of Basic Medical Sciences, College of Medicine, Alex Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria.Department of Chemistry/Biochemistry and Molecular Biology, Faculty of Science, Alex-Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, NigeriaDepartment of Chemistry/Biochemistry and Molecular Biology, Faculty of Science, Alex-Ekwueme Federal University, Ndufu-Alike, Ikwo, Ebonyi State, Nigeria; Department of Physiology, School of Medical Sciences, Universiti Sains Malaysia, Kelantan, MalaysiaDepartment of Plant Science and Biotechnology, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu State, NigeriaCyclophosphamide is an alkylating anticancer agent with strong efficacy; however, its clinical use is constrained because of its off-target multiple organ toxicity, and one of them is testicular injury. We assayed to explore whether garlic oil (GO) could prevent cyclophosphamide (CYP)-induced testicular oxidative stress and hormonal deficit in male rats. Rats were pretreated with GO for 21 days before a single injection of CP (50 mg/kg, ip). The total phenol and flavonoids of GO were estimated as well as its antioxidant capacity using DPPH and FRAP assays. CYP induced prominent depression in testicular activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and reduced glutathione (GSH) level, whereas levels of malondialdehyde (MDA) markedly increased and confirmed by histopathological alterations. Serum levels of testosterone, FSH and LH were considerably reduced. Interestingly, the GO supplementation attenuated the biochemical changes in the testis, enhanced the hormone levels and alleviated the histological injury. The IC50 of GO in DPPH assay was comparable to that of standard. GO is capable of protecting the testis from CYP toxicity via its antioxidant property. The findings suggest GO beneficial effects in male cancer patients undergoing CYP chemotherapy. Keywords: Garlic oil, Cyclophosphamide, Testosterone, Supplementation, Antioxidanthttp://www.sciencedirect.com/science/article/pii/S2352385920300013 |
| spellingShingle | Chima A. Ekeleme-Egedigwe Ademola C. Famurewa Ebuka E. David Chinedum O. Eleazu Uchenna O. Egedigwe Antioxidant potential of garlic oil supplementation prevents cyclophosphamide-induced oxidative testicular damage and endocrine depletion in rats Journal of Nutrition & Intermediary Metabolism |
| title | Antioxidant potential of garlic oil supplementation prevents cyclophosphamide-induced oxidative testicular damage and endocrine depletion in rats |
| title_full | Antioxidant potential of garlic oil supplementation prevents cyclophosphamide-induced oxidative testicular damage and endocrine depletion in rats |
| title_fullStr | Antioxidant potential of garlic oil supplementation prevents cyclophosphamide-induced oxidative testicular damage and endocrine depletion in rats |
| title_full_unstemmed | Antioxidant potential of garlic oil supplementation prevents cyclophosphamide-induced oxidative testicular damage and endocrine depletion in rats |
| title_short | Antioxidant potential of garlic oil supplementation prevents cyclophosphamide-induced oxidative testicular damage and endocrine depletion in rats |
| title_sort | antioxidant potential of garlic oil supplementation prevents cyclophosphamide induced oxidative testicular damage and endocrine depletion in rats |
| url | http://www.sciencedirect.com/science/article/pii/S2352385920300013 |
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