Aerobic and facultative anaerobic Klebsiella pneumoniae strains establish mutual competition and jointly promote Musca domestica development
IntroductionThe gut microenvironment in housefly harbors a rich and diverse microbial community which plays a crucial role in larval development. However, little is known about the impact of specific symbiotic bacteria on larval development as well as the composition of the indigenous gut microbiota...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-02-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1102065/full |
| _version_ | 1828013133859389440 |
|---|---|
| author | Kexin Zhang Kexin Zhang Shumin Wang Shumin Wang Dawei Yao Dawei Yao Xinyu Zhang Xinyu Zhang Qian Zhang Qian Zhang Wenjuan Liu Wenjuan Liu Ying Li Ying Li Yansong Yin Yansong Yin Sha An Sha An Ruiling Zhang Ruiling Zhang Zhong Zhang Zhong Zhang |
| author_facet | Kexin Zhang Kexin Zhang Shumin Wang Shumin Wang Dawei Yao Dawei Yao Xinyu Zhang Xinyu Zhang Qian Zhang Qian Zhang Wenjuan Liu Wenjuan Liu Ying Li Ying Li Yansong Yin Yansong Yin Sha An Sha An Ruiling Zhang Ruiling Zhang Zhong Zhang Zhong Zhang |
| author_sort | Kexin Zhang |
| collection | DOAJ |
| description | IntroductionThe gut microenvironment in housefly harbors a rich and diverse microbial community which plays a crucial role in larval development. However, little is known about the impact of specific symbiotic bacteria on larval development as well as the composition of the indigenous gut microbiota of housefly.MethodsIn the present study, two novel strains were isolated from housefly larval gut, i.e., Klebsiella pneumoniae KX (aerobe) and K. pneumoniae KY (facultative anaerobe). Moreover, the bacteriophages KXP/KYP specific for strains KX and KY were used to analyse the effects of K. pneumoniae on larval development.ResultsOur results showed that dietary supplementation with K. pneumoniae KX and KY individually promoted housefly larval growth. However, no significant synergistic effect was observed when the two bacterial strains were administered in combination. In addition, using high-throughput sequencing, it was demonstrated that the abundance of Klebsiella increased whereas that of Provincia, Serratia and Morganella decreased when housefly larvae received supplementation with K. pneumoniae KX, KY or the KX-KY mixture. Moreover, when used combined, K. pneumoniae KX/KY inhibited the growth of Pseudomonas and Providencia. When the abundance of both bacterial strains simultaneously increased, a balance in total bacterial abundance was reached.DiscussionThus, it can be assumed that strains K. pneumoniae KX and KY maintain an equilibrium to facilitate their development in housefly gut, by establishing competition but also cooperation with each other to maintain the constant composition of gut bacteria in housefly larvae. Thus, our findings highlight the essential role of K. pneumoniae in regulating the composition of the gut microbiota in insects. |
| first_indexed | 2024-04-10T09:42:13Z |
| format | Article |
| id | doaj.art-8a5f6de5a17d4c8aad9a5d4515ad38e6 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T09:42:13Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-8a5f6de5a17d4c8aad9a5d4515ad38e62023-02-17T08:34:56ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-02-011410.3389/fimmu.2023.11020651102065Aerobic and facultative anaerobic Klebsiella pneumoniae strains establish mutual competition and jointly promote Musca domestica developmentKexin Zhang0Kexin Zhang1Shumin Wang2Shumin Wang3Dawei Yao4Dawei Yao5Xinyu Zhang6Xinyu Zhang7Qian Zhang8Qian Zhang9Wenjuan Liu10Wenjuan Liu11Ying Li12Ying Li13Yansong Yin14Yansong Yin15Sha An16Sha An17Ruiling Zhang18Ruiling Zhang19Zhong Zhang20Zhong Zhang21School of Basic Medical Science, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaCollaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaSchool of Life Science, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaShandong Institute of Endocrine and Metabolic Diseases, Shandong First Medical University, Jinan, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaCollaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaCollaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaCollaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaCollaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaCollaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaCollaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaCollaborative Innovation Center for the Origin and Control of Emerging Infectious Diseases, Shandong First Medical University, Shandong Academy of Medical Sciences, Taian, Shandong, ChinaSchool of life Science, Weifang Medical University, Weifang, Shandong, ChinaMedical Science and Technology Innovation Center, The First Affiliated Hospital of Shandong First Medical University, Jinan, Shandong, ChinaIntroductionThe gut microenvironment in housefly harbors a rich and diverse microbial community which plays a crucial role in larval development. However, little is known about the impact of specific symbiotic bacteria on larval development as well as the composition of the indigenous gut microbiota of housefly.MethodsIn the present study, two novel strains were isolated from housefly larval gut, i.e., Klebsiella pneumoniae KX (aerobe) and K. pneumoniae KY (facultative anaerobe). Moreover, the bacteriophages KXP/KYP specific for strains KX and KY were used to analyse the effects of K. pneumoniae on larval development.ResultsOur results showed that dietary supplementation with K. pneumoniae KX and KY individually promoted housefly larval growth. However, no significant synergistic effect was observed when the two bacterial strains were administered in combination. In addition, using high-throughput sequencing, it was demonstrated that the abundance of Klebsiella increased whereas that of Provincia, Serratia and Morganella decreased when housefly larvae received supplementation with K. pneumoniae KX, KY or the KX-KY mixture. Moreover, when used combined, K. pneumoniae KX/KY inhibited the growth of Pseudomonas and Providencia. When the abundance of both bacterial strains simultaneously increased, a balance in total bacterial abundance was reached.DiscussionThus, it can be assumed that strains K. pneumoniae KX and KY maintain an equilibrium to facilitate their development in housefly gut, by establishing competition but also cooperation with each other to maintain the constant composition of gut bacteria in housefly larvae. Thus, our findings highlight the essential role of K. pneumoniae in regulating the composition of the gut microbiota in insects.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1102065/fullintestinal florahouseflyaerobic bacteriafacultative anaerobic bacteriaK. pneumoniaephage |
| spellingShingle | Kexin Zhang Kexin Zhang Shumin Wang Shumin Wang Dawei Yao Dawei Yao Xinyu Zhang Xinyu Zhang Qian Zhang Qian Zhang Wenjuan Liu Wenjuan Liu Ying Li Ying Li Yansong Yin Yansong Yin Sha An Sha An Ruiling Zhang Ruiling Zhang Zhong Zhang Zhong Zhang Aerobic and facultative anaerobic Klebsiella pneumoniae strains establish mutual competition and jointly promote Musca domestica development Frontiers in Immunology intestinal flora housefly aerobic bacteria facultative anaerobic bacteria K. pneumoniae phage |
| title | Aerobic and facultative anaerobic Klebsiella pneumoniae strains establish mutual competition and jointly promote Musca domestica development |
| title_full | Aerobic and facultative anaerobic Klebsiella pneumoniae strains establish mutual competition and jointly promote Musca domestica development |
| title_fullStr | Aerobic and facultative anaerobic Klebsiella pneumoniae strains establish mutual competition and jointly promote Musca domestica development |
| title_full_unstemmed | Aerobic and facultative anaerobic Klebsiella pneumoniae strains establish mutual competition and jointly promote Musca domestica development |
| title_short | Aerobic and facultative anaerobic Klebsiella pneumoniae strains establish mutual competition and jointly promote Musca domestica development |
| title_sort | aerobic and facultative anaerobic klebsiella pneumoniae strains establish mutual competition and jointly promote musca domestica development |
| topic | intestinal flora housefly aerobic bacteria facultative anaerobic bacteria K. pneumoniae phage |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1102065/full |
| work_keys_str_mv | AT kexinzhang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT kexinzhang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT shuminwang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT shuminwang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT daweiyao aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT daweiyao aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT xinyuzhang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT xinyuzhang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT qianzhang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT qianzhang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT wenjuanliu aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT wenjuanliu aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT yingli aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT yingli aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT yansongyin aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT yansongyin aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT shaan aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT shaan aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT ruilingzhang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT ruilingzhang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT zhongzhang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment AT zhongzhang aerobicandfacultativeanaerobicklebsiellapneumoniaestrainsestablishmutualcompetitionandjointlypromotemuscadomesticadevelopment |