Cerebral ischemic injury decreases α-synuclein expression in brain tissue and glutamate-exposed HT22 cells
Abstract α-Synuclein is abundantly expressed in neuronal tissue, plays an essential role in the pathogenesis of neurodegenerative disorders, and exerts a neuroprotective effect against oxidative stress. Cerebral ischemia causes severe neurological disorders and neuronal dysfunction. In this study, w...
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | Laboratory Animal Research |
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| Online Access: | http://link.springer.com/article/10.5625/lar.2017.33.3.244 |
| _version_ | 1818959566862286848 |
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| author | Phil-Ok Koh |
| author_facet | Phil-Ok Koh |
| author_sort | Phil-Ok Koh |
| collection | DOAJ |
| description | Abstract α-Synuclein is abundantly expressed in neuronal tissue, plays an essential role in the pathogenesis of neurodegenerative disorders, and exerts a neuroprotective effect against oxidative stress. Cerebral ischemia causes severe neurological disorders and neuronal dysfunction. In this study, we examined α-synuclein expression in middle cerebral artery occlusion (MCAO)-induced cerebral ischemic injury and neuronal cells damaged by glutamate treatment. MCAO surgical operation was performed on male Sprague-Dawley rats, and brain samples were isolated 24 hours after MCAO. We confirmed neurological behavior deficit, infarction area, and histopathological changes following MCAO injury. A proteomic approach and Western blot analysis demonstrated a decrease in α-synuclein in the cerebral cortices after MCAO injury. Moreover, glutamate treatment induced neuronal cell death and decreased α-synuclein expression in a hippocampal-derived cell line in a dose-dependent manner. It is known that α-synuclein regulates neuronal survival, and low levels of α-synuclein expression result in cytotoxicity. Thus, these results suggest that cerebral ischemic injury leads to a reduction in α-synuclein and consequently causes serious brain damage. |
| first_indexed | 2024-12-20T11:43:41Z |
| format | Article |
| id | doaj.art-8a605f0e398b451794d94cde5a37ce99 |
| institution | Directory Open Access Journal |
| issn | 2233-7660 |
| language | English |
| last_indexed | 2024-12-20T11:43:41Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Laboratory Animal Research |
| spelling | doaj.art-8a605f0e398b451794d94cde5a37ce992022-12-21T19:41:55ZengBMCLaboratory Animal Research2233-76602017-12-0133324425010.5625/lar.2017.33.3.244Cerebral ischemic injury decreases α-synuclein expression in brain tissue and glutamate-exposed HT22 cellsPhil-Ok Koh0Department of Anatomy, College of Veterinary Medicine, Gyeongsang National UniversityAbstract α-Synuclein is abundantly expressed in neuronal tissue, plays an essential role in the pathogenesis of neurodegenerative disorders, and exerts a neuroprotective effect against oxidative stress. Cerebral ischemia causes severe neurological disorders and neuronal dysfunction. In this study, we examined α-synuclein expression in middle cerebral artery occlusion (MCAO)-induced cerebral ischemic injury and neuronal cells damaged by glutamate treatment. MCAO surgical operation was performed on male Sprague-Dawley rats, and brain samples were isolated 24 hours after MCAO. We confirmed neurological behavior deficit, infarction area, and histopathological changes following MCAO injury. A proteomic approach and Western blot analysis demonstrated a decrease in α-synuclein in the cerebral cortices after MCAO injury. Moreover, glutamate treatment induced neuronal cell death and decreased α-synuclein expression in a hippocampal-derived cell line in a dose-dependent manner. It is known that α-synuclein regulates neuronal survival, and low levels of α-synuclein expression result in cytotoxicity. Thus, these results suggest that cerebral ischemic injury leads to a reduction in α-synuclein and consequently causes serious brain damage.http://link.springer.com/article/10.5625/lar.2017.33.3.244α-synucleincerebral ischemiahippocampal-derived cell lineMCAO |
| spellingShingle | Phil-Ok Koh Cerebral ischemic injury decreases α-synuclein expression in brain tissue and glutamate-exposed HT22 cells Laboratory Animal Research α-synuclein cerebral ischemia hippocampal-derived cell line MCAO |
| title | Cerebral ischemic injury decreases α-synuclein expression in brain tissue and glutamate-exposed HT22 cells |
| title_full | Cerebral ischemic injury decreases α-synuclein expression in brain tissue and glutamate-exposed HT22 cells |
| title_fullStr | Cerebral ischemic injury decreases α-synuclein expression in brain tissue and glutamate-exposed HT22 cells |
| title_full_unstemmed | Cerebral ischemic injury decreases α-synuclein expression in brain tissue and glutamate-exposed HT22 cells |
| title_short | Cerebral ischemic injury decreases α-synuclein expression in brain tissue and glutamate-exposed HT22 cells |
| title_sort | cerebral ischemic injury decreases α synuclein expression in brain tissue and glutamate exposed ht22 cells |
| topic | α-synuclein cerebral ischemia hippocampal-derived cell line MCAO |
| url | http://link.springer.com/article/10.5625/lar.2017.33.3.244 |
| work_keys_str_mv | AT philokkoh cerebralischemicinjurydecreasesasynucleinexpressioninbraintissueandglutamateexposedht22cells |